复合型酒精发酵促进剂在淀粉质原料酒精发酵废液回用中的应用

本文探讨添加复合型酒精发酵促进剂对淀粉质原料酒精发酵废液回用的影响,从发酵过程中酵母数、成熟醪酒份等方面进行对比,表明在废液回用生产酒精实验中添加复合型酒精发酵促进剂能使酵母在较恶劣的生存条件正常生长,保证酒精发酵的正常进行。

酿酒酵母X330高浓度发酵时耐酒精性能的初步研究

在完全合成培养基条件下,就渗透压保护剂和营养物质对一株产高浓度酒精的酿酒酵母X330高浓度发酵时耐酒精性能的影响进行了初步研究。结果表明,与渗透压相比,营养缺乏对酿酒酵母高浓度发酵时酒精耐受性能可能起着更为关键和重要的作用。发酵培养基中各营养元素对耐酒精性能的影响不同,由高到低的顺序是酵母抽提物>蛋白胨>硫酸镁>维生素C=磷酸二氢钾>氯化钙=硫酸铵。渗透压保护剂(甘氨酸和脯氨酸)能有效提高菌体酒精耐受性能。当甘氨酸添加浓度为20mmol/L或脯氨酸添加浓度为10mmol/L时,发酵终点酒精浓度最高,菌体于30℃在18%(V/V)酒精冲击下的存活率最大,且均高于对照组(未添加甘氨酸且未添加脯氨酸)水平,但甘氨酸的促进作用强于脯氨酸。

酒精气体在食品保鲜中的应用效果

酒精(乙醇)具有抗菌能力已被人们所认识,由于安全性高,且低浓度即有作用,作为食品保鲜剂,可采取直接添加、喷雾、浸渍等方法在食品中被利用.近年来,还被陆续开发出加有pH调整剂和其他抗菌剂的多种高功能性制剂.在众多的功能性酒精制剂中,有能产生酒精气体,并在食品保鲜中利用其气体型的产品.这种剂型是让酒精先吸附在硅胶等载体上,再装在用特殊薄膜所制的小袋内,被称之为粉末酒精制剂.与干燥剂和脱氧剂一样,不直接添加在食品中,而是附加在包装袋内.在对食品添加剂规则很严的同时,食品的低盐、低糖化、柔软化已成为热潮,其使用量在近年急速增长.据报导,目前日本已有好几家厂商生产和销售酒精气体发生剂,其市场销售额至1992年,已达15亿日元.同时,1989年还开发出在产生酒精气体的同时能进行脱氧的新型保鲜剂.下面以日本福罗因特产业所开发销售的酒精气体发生剂——“安敌摩尔达”的应用试验为主.对酒精气体发生剂在食品中的应用效果作一介绍.

长期饮酒及戒酒后肝损伤的变化

肝是受酒精损害的主要器官,酒精可引起脂肪肝,酒精性肝炎和肝硬变;肝细胞的超微结构线粒体、高尔基体及肝微管等也可发生病变。但是,酒精性肝损伤戒酒后的转归如何是人们十分关心的问题,目前研究的尚不充分。为此,本文对这方面进行了初步探讨。

Hepatoprotective and antioxidant effects of lycopene on non-alcoholic fatty liver disease in rat

AIM To evaluate the hepatoprotective effect of lycopene(Ly) on non-alcoholic fatty liver disease(NAFLD) in rat. METHODS A rat model of NAFLD was first established by feeding a high-fat diet for 14 wk. Sixty-five rats were randomly divided into normal group, model group and Ly treatment groups. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), triglycerides(TG), total cholesterol(TC) in serum and low density lipoproteincholesterol(LDL-C), high density lipoprotein-cholesterol(HDL-C), free fatty acid(FFA), malondialdehyde(MDA), superoxide dismutase(SOD), glutathione(GSH) in liver tissue were evaluated, respectively. While the hepatoprotective effect was also confirmed by histopathological analysis, the expression levels of TNF-α and cytochrome P450(CYP) 2E1 in rat liver were determined by immunohistochemistry analysis.RESULTS A significant decrease was observed in the levels of serum AST(2.07-fold), ALT(2.95-fold), and the blood lipid TG(2.34-fold) and TC(1.66-fold) in the dose of 20 mg/kg Ly-treated rats(P < 0.01), compared to the model group. Pretreatment with 5, 10 and 20 mg/kg of Ly significantly raised the levels of antioxidant enzyme SOD in a dose-dependent manner,to 90.95 ± 9.56, 109.52 ± 11.34 and 121.25 ± 10.68(P < 0.05, P < 0.01), as compared with the model group. Similarly, the levels of GSH were significantly increased(P < 0.05, P < 0.01) after the Ly treatment. Meanwhile, pretreatment with 5, 10 and 20 mg/kg of Ly significantly reduced MDA amount by 30.87, 45.51 and 54.49% in the liver homogenates, respectively(P < 0.01). The Ly treatment group showed significantly decreased levels of lipid products LDL-C(P < 0.05, P < 0.01), improved HDL-C level and significantly decreased content of FFA, compared to the model group(P < 0.05, P < 0.01). Furthermore, the Ly-treated group also exhibited a down-regulated TNF-α and CYP2E1 expression, decreased infiltration of liver fats and reversed histopathological changes, all in a dosedependent manner(P < 0.05, P < 0.01). CONCLUSION This study suggests that Ly has a protective effect on NAFLD, down-regulates expression of TNF-α, and that CYP2E1 may be one of the action mechanisms for Ly.

Insulin sensitizers in treatment of nonalcoholic fatty liver disease:Systematic review

AIM: To summarize the evidence available for the clinical effectiveness of insulin sensitizers in the treatment of nonalcoholic fatty liver disease (NAFLD) systematically. METHODS: Relevant articles were located using computer-assisted searches of Medline (1966-March 2006), EMBASE (1988-March 2006), CINAHL (1982-March 2003), Educational Resource Information Center (1966-March 2006), Library, Information Science & Technology Abstracts (1967-March 2006), Cochrane Database of Systematic Reviews, Database of Abstracts of Reviews of Effects (1994-2006), dissertations in ProQuest and FirstSearch databases. Manual searches were made in the abstracts from meetings of the American Gastroenterological Association (1999-2006), and the American Association for the Study of Liver Diseases (2003-2005). Studies were retrieved using the following selection criteria: (1) clinical trials using insulin sensitizers in subjects with NAFLD, (2) adult patients, (3) published as full manuscripts or abstracts, and (4) English, Spanish, German, and French languages only. Data were abstracted independently by two reviewers following standardized procedures. A face-to- face comparison of data was conducted to ensure the completeness and reliability of the abstraction process. RESULTS: Nine studies were included, six using metformin and three using thiazolidinediones. Only two studies were placebo-controlled trials. The mediansample size for all studies was 18 subjects. In the placebo-controlled trials, metformin improved insulin resistance markers and liver function tests, but not histological scores. In the single-arm trials, metformin and thiazolidinediones improved insulin resistance markers and liver function tests, and beneficial histological changes were reported. There is limited high-quality information available from which to draw categorical conclusions about the clinical use of insulin sensitizers in NAFLD.CONCLUSION: Current information indicates that the use of insulin sensitizers in NAFLD improves insulin resistance and liver function. Histological changes must be corroborated in randomized controlled trials.

Decreased accumulation of ultrasound contrast in the liver of nonalcoholic steatohepatitis rat model

AIM:To investigate the diagnosis of nonalcoholic steatohepatitis(NASH) using contrast ultrasonography in the NASH rat model.METHODS:The liver in methionine choline-deficient diet(MCDD) rats,a NASH model constructed by feeding an MCDD,was examined by contrast ultrasonography at weeks 2,4,8,12 and 16,with late phase images of contrast ultrasonography(Kupffer imaging) in which contrast enhancement was achieved by incorporation of a contrast agent by Kupffer cells(KCs),and images were compared to those in rats taking a regular chow.RESULTS:Decrease in contrast enhancement was observed first in MCDD rats at week 2.KCs were counted based on immunohistochemistry,but their numbers were not reduced and it was assumed that attenuation of contrast enhancement was attributable to reduced phagocytic activity of the KCs.CONCLUSION:It is suggested that clinical application of contrast ultrasonography may be valuable for noninvasive diagnosis of NASH.

Silymarin: An option to treat non-alcoholic fatty liver disease

We have read with a great interest the review published by Singh et al, on the treatment options in alcoholic and non-alcoholic fatty liver disease, including various new targeted therapies that are currently under investigation. Recently, we described the health effects of the Mediterranean diet associated to an antioxidant complex rich in silymarin, to improve in overweight patients anthropometric parameters, glucose and lipid metabolism and intra-hepatic fat accumulation.

Proteasome inhibitor treatment in alcoholic liver disease

Oxidative stress, generated by chronic ethanol consumption, is a major cause of hepatotoxicity and liver injury. Increased production of oxygen-derived free radicals due to ethanol metabolism by CYP2E1 is principally located in the cytoplasm and in the mitochondria, which does not only injure liver cells, but also other vital organs, such as the heart and the brain. Therefore, there is a need for better treatment to enhance the antioxidant response elements. To date, there is no established treatment to attenuate high levels of oxidative stress in the liver of alcoholic patients. To block this oxidative stress, proteasome inhibitor treatment has been found to significantly enhance the antioxidant response elements of hepatocytes exposed to ethanol. Recent studies have shown in an experimental model of alcoholic liver disease that proteasome inhibitor treatment at low dose has cytoprotective effects against ethanol-induced oxidative stress and liver steatosis. The beneficial effects of proteasome inhibitor treatment against oxidative stress occurred because antioxidant response elements （glutathione peroxidase 2, superoxide dismutase 2, glutathione synthetase, glutathione reductase, and GCLC） were upregulated when rats fed alcohol were treated with a low dose of PS-34Z （Bortezomib, Velcade）. This is an important finding because proteasome inhibitor treatment up-regulated reactive oxygen species removal and glutathione recycling enzymes, while ethanol feeding alone down-regulated these antioxidant elements. For the first time, it was shown that proteasome inhibition by a highly specific and reversible inhibitor is different from the chronic ethanol feeding-induced proteasome inhibition. As previously shown by our group, chronic ethanol feeding causes a complex dysfunction in the ubiquitin proteasome pathway, which affects the proteasome system, as well as the ubiquitination system. The beneficial effects of proteasome inhibitor treatment in alcoholic liver disease are related to proteasome inhibitor reversibility and the rebound of proteasome activity 72 h post PS-341 administration.

The clinical study of ultrasound-guided intra-hysteromyoma injection of ethyl alcohol absolute combined with HIFU in the treatment of uterine fibroids

Objective The aim of this study was to investigate the energy deposition in the target area of high intensity focused ultrasound （HIFU） with ultrasound-guided intra-hysteromyoma injection of ethyl alcohol absolute Methods Eighty patients with hysteromyomas were randomly divided into two groups： group HIFU （group H） and group HIFU combined with ullrasound-guided inlra-hysteromyoma injectionn of ethyl alcohol absolute （group E ＋ H）. Patients in group E ＋ H received an ullrasound-guided injection of absolute ethyl alcohol 1 h before HIFU treatment. The irradiation time, irradiation dose, energy efficiency factor （radiation energy required for ablation of a myoma per unit of volume）, grey variation, pain score, and adverse reactions were compared between the two groups. An independent sample t-test was used with a two- tailed P-value of 〈 0.05. Results The irradiation time, irradiation dose, and energy efficiency factor were significantly lower in group E ＋ H than those in group H （P 〈 0.05）. The hysteromyomas of patients in group E＋H appeared as dominantly hyperechoic masses, and those of patients in group H appeared as hyperechoic tissue （non- mass）. The incidence of adverse reactions and pain score were higher in group H than those in group E ＋ H （P 〈 0.05）. Conclusion Ultrasound-guided inlra-hysteromyoma injection of ethyl alcohol absolute can enhance the energy deposition of HIFU in the uterine fibroids, improve the treatment efficiency, shorten the treatment time, reduce the treatment dose, and reduce the pain and complications for the patients.

Alkylation of Catechol with tert-Butyl Alcohol Catalyzed by Mesoporous Acidic Montmorillonite Heterostructure Catalysts

The liquid phase alkylation of catechol with tert-butyl alcohol to produce4-tert-butyl catechol (4-TBC) was carried out over MCM-41, HZSM-5, H-exchanged montmorillonite andnovel acidic porous montmorillonite heterostructures (PMHs). Upon all catalysts tested, 4-TBC is themain product and 3-tert-butyl catechol (3-TBC) and 3,5-di-tert-butyl catechol are the sideproducts. The synthetic PMHs showed higher conversion of catechol and better selectivity to 4-TBCcompared to other solid acid catalysts tested. Over the PMHs derived from H-exchangedmontmorillonite through template extraction processes, the suitable reaction temperature is ca 410K, the ratio of catechol to tert-butyl alcohol is 1:2. Increasing the amount of catalyst (lowerweight hourly space velocity) can improve the conversion of catechol and influence the selectivityslightly. The reasonable reaction time is ca 8 h. The type and strength of acidity ofH-montmorillonite and PMH were determined by pyridine adsorption FT-IR and ammoniatemperature-programmed desorption techniques. The medium and strong acid sites are conducive toproducing 4-TBC and the weak acid sites to facilitating the 3-TBC formation. The differences betweenthe PMHs from calcination and those from extraction are attributed to proton migration and aciditychange in the gallery surface.

Effect of MS （Methylated Spirit） as a Disinfectant and Antisticking Agent on Hatchability of C/arias gariepinus Eggs and Survival of the Hatchlings

African catfish Clarias gariepinus brood stocks were bred by normal induced breeding method, After fertilization, the eggs were treated with 0.40%, 0.50% and 0.60% concentration levels of MS （methylated spirit） and a control treatment without MS （0%）. The objective was to remove the stickiness of the egg outer vitelline membrane to improve hatchability and survival of the hatchlings. Two batches of the treatments were carried out according to treatment duration of 5 s and l0 s. The eggs hatched normally with the highest hatching percentage of 62.31% in eggs treated with 0.40% MS concentration in 5 s treatment and 61.92% in the same concentration for 10 s. The control treatment of 0.00% MS treatment gave the lowest hatchability of 49.69% at 5 s and 45.71% at 10 s exposure time. Growth performance of the hatchlings improved in eggs treated than those not treated. Those treated had higher weight gain and percentage specific growth rates than those not treated. Percentage survival ranged from 75% to 90 % in both treated and untreated groups. MS can therefore be safely used in fish hatchery to prevent egg stickiness to improve hatchability and larval development.

Non-Alcohol Route of Biodiesel Synthesis from Fried Palm Oil using Immobilized Candida rugosa Lipase

Synthesis biodiesel using biocatalyst is an emerging and attracting alternative process to replace the conventional process. However, biocatalyst is easy to be deactivated by alcohol, which is a reactant in biodiesel synthesis reaction. Therefore, it is needed to develop new method to maintain the activity and stability of the biocatalyst during reaction. New method to be developed is by changing the reaction route which is using alcohol to the reaction route which is not using alcohol. Route reaction of non alcohol can be done by changing the alkyl alcohol with alkyl acetate. Both have the same function as alkyl supplier during the reaction. In this research, methyl acetate was reacted with triglyceride from fried palm oil using Candida rugosa lipase in batch reactor. The reactants and products were analyzed using HPLC. The effect of operating factors such as enzyme concentration, substrates ratio, operating temperature and addition of inhibitor using free and immobilized enzyme were investigated. The experimental results showed that 89.6% of triglyceride from fried palm oil was converted to its corresponding methyl esters under the condition of 4% wt lipase based on substrate weight, 1/12 mol rasio of oil/methyl acetate after 50 hours reaction using immobilized lipase. Stability test indicated that the activity of the immobilized biocatalyst was still remained after three reaction cycles.

Composition of Trans-anethol and Other Aromatic Volatiles in Anisated Alcoholic Beverages by Head-Space GC-MS Chromatography

Aniseed distillates, like ouzo in Greece, raki in Turkey, pastis in France, and arak in Syria, are very popular in Mediterranean countries. This flavoured alcoholic beverage is produced by extraction and distillation of fermented grape pomaces with herbal seeds from aromatic plants like Pimpinella anisum L., Foeniculum vulgate, Illicium verum and other plants which are also characterized by the presence of congeners which arise during fermentation process. Trans-anethol and its isomer cis-anethol are the main volatiles and are responsible for aroma properties of these traditional aniseed distillates. The aim of this research work was to find the concentration of special aromatic agent, trans-anethol and cis-anethol and also the major aromatic substances which are responsible for the aromatic quality. The majority of their identified components are isolated by extraction and distillation from different commercial brands and homemade samples. The constituents were identified by GC-MS （gas chromatography-mass spectometry） in this traditional spirit. The major constituents in distilled ouzo extract were also trans-anethol.

Antiarthritic Activity of Apigenin, Scutellarein and Pectinolinergenin Glycosides from Clerodendron inerme

The present study was aimed to investigate the anti-arthritic activity of alcoholic extract ofClerodendron inerme which was shown to inhibit inflammatory process. Arthritis was induced in rats by injecting rats intradermally with Freund＇s adjuvant 0.01%-0.1% in paraffin oil. They were grouped and received 10 mg/100 gm b.wt. of alcoholic fraction of C. inerme, 1.5 mg/100 gm b.wt. hydrocortisone and saline, orally, for 15 days. The arthritic scores, radiological scores, body weights, liver glycogen, serum mucoproteins were monitored for 49 days. Wilcoxan ranking methods and Student＇s t Test were used to determine the significance changes between control and treated groups. Adjuvant-arthritic rats showed a significant decrease in body weight, liver glycogen, increased serum mucoprotein and severity of periostitis and other degenerative changes m the bone. But treatment with the alcoholic fraction （apigenin, scutallarin and pectinolinergenin） of C. inerme exhibited a very good relief from the arthritic conditions by increasing the body weight, increased liver glycogen content and registered little degenerative changes in bones, suggesting long term anti-arthritic activity when compared to the control group. The anti-arthritic activity ofC. inetme alcoholic fraction was remarkable in this study. However, further studies are required to elucidate its full anti-arthritic potential.

Incretin-based therapies for type 2 diabetes with nonalcoholic fatty liver disease: a systematic review and meta-analysis

We conducted a systematic review and meta-analysis of randomized controlled trials（RCTs） to determine the effectiveness and safety of incretin-based therapies（IBTs） for the treatment of type 2 diabetes（T2DM） with nonalcoholic fatty liver disease（NAFLD）. Electronic databases such as the Cochrane library, EMbase, Pub Med, and three Chinese databases were searched for RCTs that compared IBTs with other treatments or placebo for T2 DM with NAFLD. Two reviewers independently assessed the risk of bias, extracted, and analyzed the data. A meta-analysis was performed using Revman 5.2. Publication bias was evaluated. Seven RCTs involving 532 patients were ultimately included. The results of meta-analysis（random-effects model） revealed that IBTs had a significant reduction in serum ALT（WMD –12.30, 95% CI –17.53~–7.06） and BMI（WMD –2.64, 95% CI –4.35~–0.94）. However, there was no significant difference in other outcomes including Hb A1 c, AST, TC, TG and HOMA-RA. IBTs were well tolerated by patients but the evidence was limited. The significant decrease in hepatic biochemical markers following treatment with IBTs, as well as improvements in BMI, suggested that IBTs may be an effective option for T2 DM with NAFLD.