Organic biomass is an attractive feedstock for second generation alcohol production. Wild-type strains of the genus Candida showed capabilities different to produce alcohol fermenting a carbohydrates mixture （synthet...Organic biomass is an attractive feedstock for second generation alcohol production. Wild-type strains of the genus Candida showed capabilities different to produce alcohol fermenting a carbohydrates mixture （synthetic medium）, individually and in co-culture. Therefore, the main objective of this work was to evaluate the capability of Candida wild-type strains isolated from termite gut and rumen liquid, to ferment the most commonly carbohydrates presented in citrus residues, individually and in co-culture to alcohol production. C Tropicalis （LR4） presented higher percentage of carbohydrate consumption （74.20% ± 4.60%）, alcohol production （44.53 ± 0.01 gLl） and maximal alcohol productivity （6.40 ± 0.01 gL-l day） than C Glabrata （T1）. Co-culture schemas, CC1 （LR4： 60%; TI： 40%） and CC3 （first LR4 alone and 2 days later T1） presented the highest alcohol production （45.20 ± 1.30 gL-1 and 46.80 ± 2.60 gL-1, respectively）. Maximal alcohol productivity was obtained with CC2 （LR4： 80%; TI： 20%） and CC3 schemas, 7.70 ± 0.29 gL-1 day and 7.80 ± 0.44 gL-l day, respectively. The results suggest the usefulness of these wild-type strains in co-culture as an alternative to alcohol production from carbohydrates mixtures at concentrations commonly found in citrus waste.展开更多
OBJECTIVE: To investigate the effects on non-alcoholic fatty liver disease (NAFLD) in rats of the decoction of Yiqihuoxue formula and the solution prepared with the extracts from the individual herbal medicines of the...OBJECTIVE: To investigate the effects on non-alcoholic fatty liver disease (NAFLD) in rats of the decoction of Yiqihuoxue formula and the solution prepared with the extracts from the individual herbal medicines of the formula. METHODS: The rat models of NAFLD were established with high-fat diet (HFD) for 10 weeks. Thirty-two rats were randomly divided into 4 groups: the control group, the model group, the decoction group and the solution group, 8 for each group. From the 6th week, drinking water, the decoction and the solution were intragastrically administrated accordingly to the rats for 5 weeks. The pathological changes of the liver tissues were observed with Hematoxylin and eosin staining, triglyceride levels in liver tissues measured, serum alanine aminotransferase (ALT) activity measured, and serum gastrin and motilin tested. RESULTS: Fatty degeneration and vacuole-like changes to various degrees occurred in hepaticcells of the model group. Indicators for fat metabolism, serum ALT activity and hepatic triglyceride level significantly increased, while serum gastrin and motilin levels significantly decreased. Serum ALT activity and the fatty deposition in hepatocytes were significantly reduced. In the meantime, the expressions of gastrin and motilin in the serum rose significantly in the treatment groups. CONCLUSION: Both the decoction and the extracts-mixed solution had effect on NAFLD of protecting the liver function and reducing the fatty deposition in liver, which might be achieved by regulating the expression of gastrin and motilin.展开更多
文摘Organic biomass is an attractive feedstock for second generation alcohol production. Wild-type strains of the genus Candida showed capabilities different to produce alcohol fermenting a carbohydrates mixture （synthetic medium）, individually and in co-culture. Therefore, the main objective of this work was to evaluate the capability of Candida wild-type strains isolated from termite gut and rumen liquid, to ferment the most commonly carbohydrates presented in citrus residues, individually and in co-culture to alcohol production. C Tropicalis （LR4） presented higher percentage of carbohydrate consumption （74.20% ± 4.60%）, alcohol production （44.53 ± 0.01 gLl） and maximal alcohol productivity （6.40 ± 0.01 gL-l day） than C Glabrata （T1）. Co-culture schemas, CC1 （LR4： 60%; TI： 40%） and CC3 （first LR4 alone and 2 days later T1） presented the highest alcohol production （45.20 ± 1.30 gL-1 and 46.80 ± 2.60 gL-1, respectively）. Maximal alcohol productivity was obtained with CC2 （LR4： 80%; TI： 20%） and CC3 schemas, 7.70 ± 0.29 gL-1 day and 7.80 ± 0.44 gL-l day, respectively. The results suggest the usefulness of these wild-type strains in co-culture as an alternative to alcohol production from carbohydrates mixtures at concentrations commonly found in citrus waste.
基金Supported by National Natural Science Foundation of China(No.81274155)Xiamen City Key Science and Technique Plan(No.3502Z20100006)Enterprise Project(No.XDHT2011366A)
文摘OBJECTIVE: To investigate the effects on non-alcoholic fatty liver disease (NAFLD) in rats of the decoction of Yiqihuoxue formula and the solution prepared with the extracts from the individual herbal medicines of the formula. METHODS: The rat models of NAFLD were established with high-fat diet (HFD) for 10 weeks. Thirty-two rats were randomly divided into 4 groups: the control group, the model group, the decoction group and the solution group, 8 for each group. From the 6th week, drinking water, the decoction and the solution were intragastrically administrated accordingly to the rats for 5 weeks. The pathological changes of the liver tissues were observed with Hematoxylin and eosin staining, triglyceride levels in liver tissues measured, serum alanine aminotransferase (ALT) activity measured, and serum gastrin and motilin tested. RESULTS: Fatty degeneration and vacuole-like changes to various degrees occurred in hepaticcells of the model group. Indicators for fat metabolism, serum ALT activity and hepatic triglyceride level significantly increased, while serum gastrin and motilin levels significantly decreased. Serum ALT activity and the fatty deposition in hepatocytes were significantly reduced. In the meantime, the expressions of gastrin and motilin in the serum rose significantly in the treatment groups. CONCLUSION: Both the decoction and the extracts-mixed solution had effect on NAFLD of protecting the liver function and reducing the fatty deposition in liver, which might be achieved by regulating the expression of gastrin and motilin.
