Purpose： To extract protein, decrease the cellulose and facilitate the digestion and absorption of brewers＇ spent grain by animal. Topic： Discuss and optimize the hydrolysis conditions of the combined enzymatic hyd...Purpose： To extract protein, decrease the cellulose and facilitate the digestion and absorption of brewers＇ spent grain by animal. Topic： Discuss and optimize the hydrolysis conditions of the combined enzymatic hydrolysis by Novozymes. Method： The fresh brewers＇ spent grain was firstly dried, smashed and sifted. Then as indicators of the protein extraction rate in the enzyme solution and the content of cellulose in the index, the parameters of enzymatic hydrolysis, such as the solid-liquid ratio, reaction temperature, pH, enzyme dosage and reaction time, were investigated in detailed. After hydrolysis, the brewers＇ spent grain was put in the boiling water bath for inactivation for 15 minutes, and centrifuged, the supernatants were volume to 100 mL and the protein content was measured. After the precipitate was dried, the cellulose content was also measured. Achievements： The optimized conditions were with temperature of 50 ℃, pH 6.5, enzyme amount of 30 mg for Novozymes enzyme and 2.5 h for reaction time. Under these conditions, the protein extraction rate in the enzyme reaction reached 41.82%, and the cellulose content reached 13.90%, the degradation rate of cellulose was 18.86%.展开更多
The present experiment was focused on quality of dry distillers＇ grains with solubles （DDGS） produced from corn （DDGSc）, wheat （DDGSw）, triticale （DDGSt）, and wet distillers grains from corn （WDGc） for rumi...The present experiment was focused on quality of dry distillers＇ grains with solubles （DDGS） produced from corn （DDGSc）, wheat （DDGSw）, triticale （DDGSt）, and wet distillers grains from corn （WDGc） for ruminants. The products were tested for dry matter （DM）, crude protein （CP）, neutral detergent fibre （NDF）, acid detergent fibre （ADF） acid detergent insoluble nitrogen （NIAD）, amino acid （AA） profile, CP degradability and intestinal digestibility （ID） of rumen undegraded protein （RUP）. The average content of CP （g/kg DM） ranged from 289.9 （DDGSc） to 356.9 （DDGSw）. The highest content of essential AA was in WDGc （440.1 g/160g N） followed with DDGSc. The most variable was the content of Lys in DDGSw （from 13.69 to 22.79 g/160 g N）. The NIAD was found the highest for DDGSw （from 16.8% to 36.2%）, effective CP degradability was only 50.2% and ID of RUP 82.2%, total essential AA 81.2%, Lys 77.69% and Met 81.4%. Lys was generally the least digestible AA. The ruminally degradable CP fraction was in the range from 52.6% to 70.2% for DDGSc. ID of total and essential by-pass AA was very high 96.2% and 96.1%, Lysine 90.9% and Methionine 96.7%. From these data it can be concluded that DDGS from grains are a good source of protein for ruminants.展开更多
文摘Purpose： To extract protein, decrease the cellulose and facilitate the digestion and absorption of brewers＇ spent grain by animal. Topic： Discuss and optimize the hydrolysis conditions of the combined enzymatic hydrolysis by Novozymes. Method： The fresh brewers＇ spent grain was firstly dried, smashed and sifted. Then as indicators of the protein extraction rate in the enzyme solution and the content of cellulose in the index, the parameters of enzymatic hydrolysis, such as the solid-liquid ratio, reaction temperature, pH, enzyme dosage and reaction time, were investigated in detailed. After hydrolysis, the brewers＇ spent grain was put in the boiling water bath for inactivation for 15 minutes, and centrifuged, the supernatants were volume to 100 mL and the protein content was measured. After the precipitate was dried, the cellulose content was also measured. Achievements： The optimized conditions were with temperature of 50 ℃, pH 6.5, enzyme amount of 30 mg for Novozymes enzyme and 2.5 h for reaction time. Under these conditions, the protein extraction rate in the enzyme reaction reached 41.82%, and the cellulose content reached 13.90%, the degradation rate of cellulose was 18.86%.
文摘The present experiment was focused on quality of dry distillers＇ grains with solubles （DDGS） produced from corn （DDGSc）, wheat （DDGSw）, triticale （DDGSt）, and wet distillers grains from corn （WDGc） for ruminants. The products were tested for dry matter （DM）, crude protein （CP）, neutral detergent fibre （NDF）, acid detergent fibre （ADF） acid detergent insoluble nitrogen （NIAD）, amino acid （AA） profile, CP degradability and intestinal digestibility （ID） of rumen undegraded protein （RUP）. The average content of CP （g/kg DM） ranged from 289.9 （DDGSc） to 356.9 （DDGSw）. The highest content of essential AA was in WDGc （440.1 g/160g N） followed with DDGSc. The most variable was the content of Lys in DDGSw （from 13.69 to 22.79 g/160 g N）. The NIAD was found the highest for DDGSw （from 16.8% to 36.2%）, effective CP degradability was only 50.2% and ID of RUP 82.2%, total essential AA 81.2%, Lys 77.69% and Met 81.4%. Lys was generally the least digestible AA. The ruminally degradable CP fraction was in the range from 52.6% to 70.2% for DDGSc. ID of total and essential by-pass AA was very high 96.2% and 96.1%, Lysine 90.9% and Methionine 96.7%. From these data it can be concluded that DDGS from grains are a good source of protein for ruminants.
