A scheme of investigating the intracellular metabolic fluxes in central metabolism of Saccharomyces cerevisiae based on isotope model and tracer experiment was developed. The metabolic model applied in this study incl...A scheme of investigating the intracellular metabolic fluxes in central metabolism of Saccharomyces cerevisiae based on isotope model and tracer experiment was developed. The metabolic model applied in this study includes the Embden-Meyerhof-Parnas pathway,the pentose phosphate pathway,the tricarboxylic acid cycle,CO2 anaplerotic reactions,ethanol and acetate formation,and pathways involved in amino acid synthesis. The approach of hybridized genetic algorithm combined with the sequential simplex technique was used to optimize a quadratic error function without the requirement of the information on the partial derivatives. The impact of some key pa-rameters on the algorithm was studied. This approach was proved to be rapid and numerically stable in the analysis of the central metabolism of S.cerevisiae.展开更多
Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX （alcohol oxidase） protein which has been c...Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX （alcohol oxidase） protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabolomics is the systematic study and inclusive analysis of small molecules called metabolites in a biological system. Metabolomics plays an important part in connecting the phenotype and genotype gap because it magnifies the modifications in the proteome and provides a better phenotype representation of an organism. This quantitative study has provided a new perception on the metabolic burden derived from the overexpression of recombinant protein in methylotrophic yeast. In this review, we discuss the fundamental aspect of metabolomics in methylotrophic yeast followed by their latest developments.展开更多
沉默信息调节因子1（silent information regulator 1,SIRT1）,哺乳动物sirtuins家族中的一种去乙酰化酶,对组蛋白及多种细胞转录调控因子具有非常重要的作用,可参与细胞凋亡、代谢、线粒体生物合成、自噬等过程的调节,并在多种疾病,如...沉默信息调节因子1（silent information regulator 1,SIRT1）,哺乳动物sirtuins家族中的一种去乙酰化酶,对组蛋白及多种细胞转录调控因子具有非常重要的作用,可参与细胞凋亡、代谢、线粒体生物合成、自噬等过程的调节,并在多种疾病,如神经退行性变,心血管疾病,代谢性疾病,肿瘤以及衰老相关性疾病中发挥保护作用.随着SIRT1在肾脏领域研究的不断深入,其在肾脏生理病理过程中的作用日益受到关注,SIRT1有望成为肾脏疾病的潜在治疗靶点.展开更多
基金Supported by the National Natural Science Foundation of China (No.20276065)the Special Funds for Major State BasicResearch Program of China (973 Program, 2007CB707805).
文摘A scheme of investigating the intracellular metabolic fluxes in central metabolism of Saccharomyces cerevisiae based on isotope model and tracer experiment was developed. The metabolic model applied in this study includes the Embden-Meyerhof-Parnas pathway,the pentose phosphate pathway,the tricarboxylic acid cycle,CO2 anaplerotic reactions,ethanol and acetate formation,and pathways involved in amino acid synthesis. The approach of hybridized genetic algorithm combined with the sequential simplex technique was used to optimize a quadratic error function without the requirement of the information on the partial derivatives. The impact of some key pa-rameters on the algorithm was studied. This approach was proved to be rapid and numerically stable in the analysis of the central metabolism of S.cerevisiae.
文摘Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX （alcohol oxidase） protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabolomics is the systematic study and inclusive analysis of small molecules called metabolites in a biological system. Metabolomics plays an important part in connecting the phenotype and genotype gap because it magnifies the modifications in the proteome and provides a better phenotype representation of an organism. This quantitative study has provided a new perception on the metabolic burden derived from the overexpression of recombinant protein in methylotrophic yeast. In this review, we discuss the fundamental aspect of metabolomics in methylotrophic yeast followed by their latest developments.
文摘沉默信息调节因子1（silent information regulator 1,SIRT1）,哺乳动物sirtuins家族中的一种去乙酰化酶,对组蛋白及多种细胞转录调控因子具有非常重要的作用,可参与细胞凋亡、代谢、线粒体生物合成、自噬等过程的调节,并在多种疾病,如神经退行性变,心血管疾病,代谢性疾病,肿瘤以及衰老相关性疾病中发挥保护作用.随着SIRT1在肾脏领域研究的不断深入,其在肾脏生理病理过程中的作用日益受到关注,SIRT1有望成为肾脏疾病的潜在治疗靶点.
