最近研究表明,磷酸甘油酸激酶1(PGK1)与肿瘤的发生和发展密切相关。PGK1在多个位点上发生磷酸化和乙酰化,在特定条件下会发生线粒体和细胞核的易位,直接或间接地增强糖酵解活性,促进肿瘤细胞的增殖。2020年1月7日,易文教授团队在《自然...最近研究表明,磷酸甘油酸激酶1(PGK1)与肿瘤的发生和发展密切相关。PGK1在多个位点上发生磷酸化和乙酰化,在特定条件下会发生线粒体和细胞核的易位,直接或间接地增强糖酵解活性,促进肿瘤细胞的增殖。2020年1月7日,易文教授团队在《自然通讯》(Nature Communications)在线发表了题为“0-GlcNAcylation of PGK1 coordinates glycolysis and TCA cyele to promote tumor growth”的研究论文(hps://www.nature.com/articles/s41467-019-136018),揭示了PGK1在蛋白质的丝氨酸或苏氨酸残基上进行糖基化修饰(0-GleNAc)中的功能:一方面,糖基化修饰可上调PGK1的代谢酶活性,增强糖酵解作用;另一方面,糖基化修饰可诱导PGK1发生线粒体易位,进而抑制三羧酸循环代谢。这两种功能的叠加能增强肿瘤细胞的瓦尔堡效应,从而促进肿瘤的生长。展开更多
Ceramides are a class of lipid molecules widely distributed in eukaryotic cells in small amount. To investigate the possibility of ceramide production by yeast, a yeast strain Yarrowia lipolitica was grown under diffe...Ceramides are a class of lipid molecules widely distributed in eukaryotic cells in small amount. To investigate the possibility of ceramide production by yeast, a yeast strain Yarrowia lipolitica was grown under different conditions including changing carbon/nitrogen ratio, and serine concentration, dissolved oxygen and presence of ethanol. It was found that increased dissolved oxygen supply increased the ceramide content in the yeast 2.5 fold of its normal control level. Ethanol treatment could also enhance ceramide accumulation by 3.3 fold compared with the control although the cell growth was negatively affected. Cellular redox potential was shown to affect ceramide accumulation by the yeast. This was possibly related to the cellular reactive oxygen species presented in the yeast.展开更多
文摘最近研究表明,磷酸甘油酸激酶1(PGK1)与肿瘤的发生和发展密切相关。PGK1在多个位点上发生磷酸化和乙酰化,在特定条件下会发生线粒体和细胞核的易位,直接或间接地增强糖酵解活性,促进肿瘤细胞的增殖。2020年1月7日,易文教授团队在《自然通讯》(Nature Communications)在线发表了题为“0-GlcNAcylation of PGK1 coordinates glycolysis and TCA cyele to promote tumor growth”的研究论文(hps://www.nature.com/articles/s41467-019-136018),揭示了PGK1在蛋白质的丝氨酸或苏氨酸残基上进行糖基化修饰(0-GleNAc)中的功能:一方面,糖基化修饰可上调PGK1的代谢酶活性,增强糖酵解作用;另一方面,糖基化修饰可诱导PGK1发生线粒体易位,进而抑制三羧酸循环代谢。这两种功能的叠加能增强肿瘤细胞的瓦尔堡效应,从而促进肿瘤的生长。
文摘Ceramides are a class of lipid molecules widely distributed in eukaryotic cells in small amount. To investigate the possibility of ceramide production by yeast, a yeast strain Yarrowia lipolitica was grown under different conditions including changing carbon/nitrogen ratio, and serine concentration, dissolved oxygen and presence of ethanol. It was found that increased dissolved oxygen supply increased the ceramide content in the yeast 2.5 fold of its normal control level. Ethanol treatment could also enhance ceramide accumulation by 3.3 fold compared with the control although the cell growth was negatively affected. Cellular redox potential was shown to affect ceramide accumulation by the yeast. This was possibly related to the cellular reactive oxygen species presented in the yeast.
