Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found i...Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group(Ctrl), spine spondylolisthesis(SSP) group, spinal fracture(SFR) group, and spine scoliosis(SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases.展开更多
Lanthanide elements(Ln)play an important role in industry and agriculture.As a result of the increasing consumption of lanthanides,environmental emission of Ln has become detrimental to the health of flora and fauna.C...Lanthanide elements(Ln)play an important role in industry and agriculture.As a result of the increasing consumption of lanthanides,environmental emission of Ln has become detrimental to the health of flora and fauna.Current methods for trace lanthanides detection mainly rely on sophisticated instruments.In this article,a Ln^(3+)dependent DNAzyme was incorporated into a hydrogel to generate Ln^(3+)sensitive DNAzyme hydrogel for portable colorimetric detection.The enzyme strand and its substrate strand act as crosslinker and functional unit of the hydrogel with polyacrylamide chains as the scaffold and gold nanoparticles(AuNPs)as the indicator of hydrogel stability.Any ions in the Ln^(3+)series can trigger the cleavage of substrate strand by activating the enzyme strand,thereby decreasing the crosslink ratio and leading to collapse of the hydrogel.The release of the encapsulated AuNPs turns the supernatant wine red.Using this colorimetric method,Ln^(3+)can be detected with high sensitivity,with a limit of detection(LOD)of 20 nM for Ce^(3+).The hydrogel responds specifically to any Ln^(3+)ion and works well with the spiked lake sample without the need of instruments and skilled operators.Our results suggest that the lanthanide responsive hydrogel can be used for portable and sensitive detection of Ln^(3+)contamination in the field.展开更多
基金supported by the National Basic Research Program of China(2012CB933301)the Key Discipline Construction Project of Pudong Health Bureau of Shanghai(PWZx2014-02)+1 种基金the Sci-tech Support Plan of Jiangsu Province(BE2014719)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group(Ctrl), spine spondylolisthesis(SSP) group, spinal fracture(SFR) group, and spine scoliosis(SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases.
基金supported by the National Basic Research Program of China(2013CB933703)the National Natural Science Foundation of China(91313302,21205100,21275122,21075104)+2 种基金the National Instrumentation Program(2011YQ03012412)the National Found for Fostering Talents of Basic Science(J1310024)the National Science Foundation for Distinguished Young Scholars(21325522)
文摘Lanthanide elements(Ln)play an important role in industry and agriculture.As a result of the increasing consumption of lanthanides,environmental emission of Ln has become detrimental to the health of flora and fauna.Current methods for trace lanthanides detection mainly rely on sophisticated instruments.In this article,a Ln^(3+)dependent DNAzyme was incorporated into a hydrogel to generate Ln^(3+)sensitive DNAzyme hydrogel for portable colorimetric detection.The enzyme strand and its substrate strand act as crosslinker and functional unit of the hydrogel with polyacrylamide chains as the scaffold and gold nanoparticles(AuNPs)as the indicator of hydrogel stability.Any ions in the Ln^(3+)series can trigger the cleavage of substrate strand by activating the enzyme strand,thereby decreasing the crosslink ratio and leading to collapse of the hydrogel.The release of the encapsulated AuNPs turns the supernatant wine red.Using this colorimetric method,Ln^(3+)can be detected with high sensitivity,with a limit of detection(LOD)of 20 nM for Ce^(3+).The hydrogel responds specifically to any Ln^(3+)ion and works well with the spiked lake sample without the need of instruments and skilled operators.Our results suggest that the lanthanide responsive hydrogel can be used for portable and sensitive detection of Ln^(3+)contamination in the field.
