乙型肝炎病毒核酸DNA定量检测与标志物酶免检测的相关性分析

目的探讨乙型肝炎病毒各标志物与乙型肝炎病毒核酸(DNA)之间的关系,为乙型肝炎病毒临床检测和患者疗效观察的监测提出检测方法学的建议。方法对134例乙型肝炎患者血清用科华酶免试剂作HBsAg、HBeAg、HBeAb、Pre-S1四项检测,同时用中山达安DA5700基因扩增仪和试剂做乙型肝炎病毒DNA核酸扩增定量检测,按乙型肝炎病毒DNA核酸定量检测扩增数量级分组,每一级为一组,共分6组,然后与科华酶免试剂的HBsAg、HBeAg、HBeAb、Pre-S1四项检测结果分别作统计学分析。结果 134例乙型肝炎病毒患者血清DNA核酸扩增<1×103有34例,>1×103有100例,阳性率74.6%;各组分别与DNA<1×103组比较,DNA扩增项DNA>1×103的各组有极显著性差异(P<0.01);HbsAg项各组均无显著性差异(P>0.05);HBeAg项、HBeAb项、Pre-S项DNA>1×106组和DNA>1×107组有极显著性差异(P<0.01),其余各组无显著性差异(P>0.05)。各项之间作相关性分析结果为乙型肝炎DNA检测项与HBsAg项(r=0.1517,P>0.05)无相关性;DNA检测项与HBeAg、HBeAb、Pre-S1相关性分别为(r=0.7852,r=-0.6143,r=0.6260;P均<0.05),有相关性。结论乙型肝炎病毒核酸DNA定量检测项各组之间存在显著性差异;乙型肝炎病毒核酸DNA载量>1×105以上组与HbeAg、HBeAb、Pre-S1有相关性。

Incidence of toxoplasmosis in patients with cirrhosis

AIM:It is known that toxoplasmosis rarely leads to various liver pathologies,most common of which is granulomatose hepatitis in patients having normal immune systems.Patients who have cirrhosis of the liver are subject to a variety of cellular as well as humoral immunity disorders.Therefore,it may be considered that toxoplasmosis can cause more frequent and more severe diseases in patients with cirrhosis and is capable of changing the course of the disease.The aim of this study was to investigate the frequency of toxoplasmosis in patients with cirrhosis. METHODS:Serum samples were taken from 108 patients with cirrhosis under observation in the Hepatology Polyclinic of the Gastroenterology Clinic,and a control group made up of 50 healthy blood donors.IFAT and ELISA methods were used to investigate the IgG and IgM antibodies,which had developed from these sera. RESULTS:Toxoplasma IgG and IgN antibody positivity was found in 74 (68.5%) of the 108 cirrhotic patients and 24 (48%) of the 50 people in the control group.The difference between them was significant (P<0.05). CONCLUSION:In conclusion,it was found that the toxopiasma sero-prevalence in the cirrhotic patients in this study was higher.Cirrhotic patients are likely to form a toxoplasma risk group.More detailed studies are needed on this subject.

Influence of acupuncture on cognitive function and markers of oxidative DNA damage in patients with vascular dementia

OBJECTIVE:To test the influence of acupuncture on cognitive function and a marker of oxidative DNA damage in patients with vascular dementia(VD).METHODS:Sixteen VD patients were evaluated before and after acupuncture,using the Folstein Mini-Mental State Examination-Revised(MMSE-R) to assess cognitive function,and the ADL-R scale to assess independence in activities of daily living(ADL).Life quality was evaluated using the DEMQOL(Dementia quality of life questionnaire) questionnaire,and syndromes and expression of vascular dementia were evaluated with the Scale for the Differentiation of Syndromes of Vascular Dementia(SDSVD).In addition,the urine concentration of 8-hydroxy-2'-deoxyguanosine(8-OHdG)-a marker of oxidative damage-was quantified with enzyme-linked immunosorbent assay.RESULTS:The MMSE-R and DEMQOL scores were higher after acupuncture than before(P<0.05),while there were no obvious differences in the ADL-R or SDSVD scores(P>0.05).The 8-OHdG content in urine significantly decreased after acupuncture(P<0.05).CONCLUSION:Acupuncture reduces the levels of 8-OHdG and improves cognitive function and quality of life in VD patients,suggesting that acupuncture is beneficial at least in part by preventing oxidative damage.

Colorimetric detection and efficient monitoring of a potential biomarker of lumbar disc herniation using carbon nanotube-based probe

Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group(Ctrl), spine spondylolisthesis(SSP) group, spinal fracture(SFR) group, and spine scoliosis(SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases.