AIM： TO determine whether -1195 A→G and/or -765 G→C polymorphisms in Cyclooxygenase-2 CCOX-2） may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population. METHODS： ...AIM： TO determine whether -1195 A→G and/or -765 G→C polymorphisms in Cyclooxygenase-2 CCOX-2） may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population. METHODS： Two study groups were recruited, 252 patients with esophageal carcinoma and 240 healthy controls, matched for race, age, gender and recruiting area. DNA was isolated from whole blood and used for genotyping. PCR products were digested with restriction enzymes and products were analyzed by agarose gel electrophoresis. Odds ratios （OR） and 95% confidence intervals （CI） were estimated. RESULTS： The distribution of the -1195A→G polymorphism was significantly different in esophageal cancer patients compared to controls. The -1195 GG genotype resulted in a higher risk of developing esophageal adenocarcinoma （OR = 3.85, 95% CI： 1.45-10.3） compared with the -1195AA genotype as a reference. The -765 G→C genotype distribution was not different between the two groups. The GG/ GG haplotype was present more often in esophageal adenocarcinoma patients than in controls （OR = 3.45, 95% CI： 1.24-9.58; with AG/AG as a reference）. The same trends were observed in patients with squamous cell carcinomas, however, the results did not reach statistical significance. CONCLUSION： Presence of the COX-2 -1195 GG genotype and of the GG/GG haplotype may result in a higher risk of developing esophageal carcinoma.展开更多
目的 在河北省传染病病原鉴定分析与流行病学重点实验室建立肺炎链球菌(Streptococcus pneumoniae,Spn)脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分型方法,为河北省肺炎链球菌引起的相关疾病的流行病学监测与调查提供可...目的 在河北省传染病病原鉴定分析与流行病学重点实验室建立肺炎链球菌(Streptococcus pneumoniae,Spn)脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分型方法,为河北省肺炎链球菌引起的相关疾病的流行病学监测与调查提供可靠的技术支撑。方法 参照美国PulseNet手册标准方法和《国家致病菌识别网技术手册》,在河北省传染病病原鉴定分析与流行病学重点实验室进行肺炎链球菌PFGE分型实验,同时对缓冲液种类、限制性核酸内切酶浓度、酶切时间和电泳条件进行优化。结果 利用优化后的条件对收集到的肺炎链球菌菌株进行PFGE分型分析,所得到的酶切电泳图谱条带清晰,分辨率高。结论 河北省传染病病原鉴定分析与流行病学重点实验室建立和优化了肺炎链球菌PFGE分型分析方法,今后可用于肺炎链球菌的分子特征分析。展开更多
文摘AIM： TO determine whether -1195 A→G and/or -765 G→C polymorphisms in Cyclooxygenase-2 CCOX-2） may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population. METHODS： Two study groups were recruited, 252 patients with esophageal carcinoma and 240 healthy controls, matched for race, age, gender and recruiting area. DNA was isolated from whole blood and used for genotyping. PCR products were digested with restriction enzymes and products were analyzed by agarose gel electrophoresis. Odds ratios （OR） and 95% confidence intervals （CI） were estimated. RESULTS： The distribution of the -1195A→G polymorphism was significantly different in esophageal cancer patients compared to controls. The -1195 GG genotype resulted in a higher risk of developing esophageal adenocarcinoma （OR = 3.85, 95% CI： 1.45-10.3） compared with the -1195AA genotype as a reference. The -765 G→C genotype distribution was not different between the two groups. The GG/ GG haplotype was present more often in esophageal adenocarcinoma patients than in controls （OR = 3.45, 95% CI： 1.24-9.58; with AG/AG as a reference）. The same trends were observed in patients with squamous cell carcinomas, however, the results did not reach statistical significance. CONCLUSION： Presence of the COX-2 -1195 GG genotype and of the GG/GG haplotype may result in a higher risk of developing esophageal carcinoma.
文摘目的 在河北省传染病病原鉴定分析与流行病学重点实验室建立肺炎链球菌(Streptococcus pneumoniae,Spn)脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分型方法,为河北省肺炎链球菌引起的相关疾病的流行病学监测与调查提供可靠的技术支撑。方法 参照美国PulseNet手册标准方法和《国家致病菌识别网技术手册》,在河北省传染病病原鉴定分析与流行病学重点实验室进行肺炎链球菌PFGE分型实验,同时对缓冲液种类、限制性核酸内切酶浓度、酶切时间和电泳条件进行优化。结果 利用优化后的条件对收集到的肺炎链球菌菌株进行PFGE分型分析,所得到的酶切电泳图谱条带清晰,分辨率高。结论 河北省传染病病原鉴定分析与流行病学重点实验室建立和优化了肺炎链球菌PFGE分型分析方法,今后可用于肺炎链球菌的分子特征分析。
