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大豆胰蛋白酶抑制因子检测方法的研究 被引量:4
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作者 殷红 程志斌 《饲料工业》 北大核心 2004年第4期53-55,共3页
关键词 饲料原料 大豆 胰蛋白抑制因子 检测 氢氧化钾检测 酶化学分析法 免疫化学测定
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Gold nanolabels and enzymatic recycling dual amplification-based electrochemical immunosensor for the highly sensitive detection of carcinoembryonic antigen
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作者 ZHANG YuYong XIANG Yun +6 位作者 CHAI YaQin YUAN Ruo QIAN XiaoQing ZHANG HaiXia CHEN Ying SU Jiao XU Jin 《Science China Chemistry》 SCIE EI CAS 2011年第11期1770-1776,共7页
A sensitive electrochemical immunoassay system for the detection of a protein tumor biomarker through a dual amplified strategy was reported. Firstly, this protocol involves in the electropolymerization of o-aminobenz... A sensitive electrochemical immunoassay system for the detection of a protein tumor biomarker through a dual amplified strategy was reported. Firstly, this protocol involves in the electropolymerization of o-aminobenzoic acid (o-ABA) on a glass carbon electrode (GCE). Subsequently, capture anti-CEA (Abl) is covalently linked to poly(o-ABA) (PAB) film, via N-(3-dimethylamminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysulfosuccinimid sodium salt (NHS) activation of the carboxyl groups and surface blocking with ethanolamine. Later, the target, carcinoembryonic antigen (CEA), is sandwiched between an electrode surface confined Ab1 and the alkaline phosphatase-labeled signal anti-CEA antibodies conjugated with gold nanoparticles (Ab2-ALP-AuNP bioconjugates). The dual biocatalytic signal amplification for CEA monitoring is achieved by coupling the numerous enzymes loaded on the AuNPs with redox-recycling of the enzymatic products in the presence of the secondary enzyme and the corresponding substrate. The novel dramatic signal amplification strategy, exhibits a good linearity at the studied concentration range from 0.005 to 50 ng mL-1 towards CEA with a detection limit of 2 pg mL-1 (S/N=3). There is a 5-100-fold improvement in detection limit compared to other similar studies. The developed dual signal amplified strategy shows good selectivity, regeneration, stability and acceptable reproducibility. Therefore, the signal amplification approach holds great potential applications in detection of ultra-trace protein biomarkers. 展开更多
关键词 AMPLIFICATION electrochemical immunosensor carcinoembryonic antigen redox-recycling
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