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耐温淀粉酶溶液蒸发浓缩过程中的质量评估
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作者 杨宗政 刘振义 《食品与发酵工业》 CAS CSCD 北大核心 2001年第2期13-17,共5页
研究了异形竖板降膜蒸发器浓缩耐温淀粉酶过程中的酶失活动力学 ,并在浓缩过程中酶失活动力学的基础上 。
关键词 蒸发浓缩 耐温淀粉 质量评估 异形竖板降膜蒸发器 酶失活动力学
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Inactivation Kinetics of β-N-Acetyl-D-Glucosaminidase from Prawn (Penaeus vannamei) by Formaldehyde 被引量:2
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作者 谢晓兰 石艳 +1 位作者 黄乾生 陈清西 《Marine Science Bulletin》 CAS 2006年第1期34-45,共12页
β-N-Acetyl-D-glucosaminidase (NAGase, EC.3.2.1.52) is chitinolytic enzymes and disintegrate dimmer and trimer a composition of oligomers of N-acetyl-β-D-glucosamine (NAG) into monomer. Prawn (P. vannamei) NAG... β-N-Acetyl-D-glucosaminidase (NAGase, EC.3.2.1.52) is chitinolytic enzymes and disintegrate dimmer and trimer a composition of oligomers of N-acetyl-β-D-glucosamine (NAG) into monomer. Prawn (P. vannamei) NAGase is involved in digestion and molting processes. Some pollutants in seawater affect the enzyme activity causing loss of the biological function of the enzyme, which affects the exuviating shell and threatens the survival of the animal. The effect of formaldehyde on prawn (P. vannamei) β-N-acetyl-D-glucosaminidase activity for the hydrolysis of pNP-NAG has been studied. The results show that formaldehyde, at appropriate concentrations, can lead to reversible inactivation of the enzyme, and the IC50 is estimated to be 1.05mol· L^-1. The inactivation mechanism obtained from Lineweaver-Burk plots shows that the inactivation of the enzyme by formaldehyde belongs to the competitive type. The inactivation kinetics of the enzyme by formaldehyde has been studied using the progress-of-substrate-reaction method described by Tsou, and the rate constants have been determined. The results show that k+0 is much larger than k-0, indicating the free enzyme molecule is fragile in the formaldehyde solution. 展开更多
关键词 P. vannamei β-N-acetyl-D-glucosaminidase inactivation kinetics FORMALDEHYDE
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Deactivation Kinetics of Nitrile Hydratase in Free Resting Cells 被引量:3
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作者 孙旭东 于慧敏 沈忠耀 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2009年第5期822-828,共7页
Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-ste... Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-steady state assumption.Effects of hydration temperature,product concentration and substrate concentration on NHase deactivation were investigated experimentally and correlated with a first order deactivation kinetics.The results showed that the hydration temperature and product concentration were major factors governing the deactivation of NHases under substrate-feeding conditions.When acrylamide concentration was higher than 250 g·L1,the deactivation of NHases became serious and the bi-steady state assumption was not applicable.When the hydration temperature was controlled at a relatively higher level such as 28°C,the total deactivation rate constant was about 2.8-fold of that at 20°C. 展开更多
关键词 nitrile hydratase deactivation kinetics free resting cell bi-steady state assumption total deactivation rate constant
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