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A型流感病毒神经氨酸酶蛋白功能研究进展 被引量:3
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作者 郑沁玫 顾敏 刘秀梵 《动物医学进展》 北大核心 2021年第10期66-70,共5页
A型流感病毒(IAV)的宿主谱广泛,对人类健康和畜禽养殖均构成严重威胁。神经氨酸酶(neuraminidase,NA)作为IAV囊膜表面主要的糖蛋白之一,在病毒的感染过程中至关重要,是常用的抗病毒药物作用靶点。以往关于NA蛋白的研究主要聚焦于其通过... A型流感病毒(IAV)的宿主谱广泛,对人类健康和畜禽养殖均构成严重威胁。神经氨酸酶(neuraminidase,NA)作为IAV囊膜表面主要的糖蛋白之一,在病毒的感染过程中至关重要,是常用的抗病毒药物作用靶点。以往关于NA蛋白的研究主要聚焦于其通过切割宿主细胞表面唾液酸与血凝素(hemagglutinin,HA)之间的α-2,6或α-2,3糖苷键以促进新生病毒粒子释放的功能方面,而近来不断有证据表明,神经氨酸酶蛋白在受体结合和宿主适应性方面同样发挥关键作用。论文综述了神经氨酸酶蛋白的结构特征,并对神经氨酸酶蛋白的酶催化活性、受体结合功能以及与HA蛋白的功能平衡方面进行了总结,以期为深入理解IAV感染的分子机制提供参考。 展开更多
关键词 A型流感病毒 神经氨酸 受体结合 唾液酸 血凝素-神经氨酸酶平衡
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“益生菌在食品加工中的应用”专题征稿函
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《食品安全质量检测学报》 CAS 2016年第12期4833-4833,共1页
益生菌是一类能够改善宿主消化道菌群及酶平衡的活性有益微生物,能够提高机体的抗病能力、代谢能力和对食物的消化吸收能力,从而达到防治消化道疾病和促进生长的双重作用。将益生菌添加到食品中得到的相关产品种类众多,因其对人体独特... 益生菌是一类能够改善宿主消化道菌群及酶平衡的活性有益微生物,能够提高机体的抗病能力、代谢能力和对食物的消化吸收能力,从而达到防治消化道疾病和促进生长的双重作用。将益生菌添加到食品中得到的相关产品种类众多,因其对人体独特的有益作用和良好的口感受到消费者的普遍欢迎。鉴于此,本刊特别策划了"益生菌在食品加工中的应用"专题,由北京工商大学食品学院的杨贞耐教授担任专题主编,主要围绕益生菌的种类、作用机制及对人体健康的影响、在食品中的添加方式、相关产品的研制等发面或您认为有意义的相关领域展开论述和研究,本专题计划在2017年3月出版。 展开更多
关键词 食品加工 征稿函 消化道疾病 消化吸收能力 北京工商大学 杨贞 酶平衡 相关产品 代谢能力 抗病能力
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血清尿素参考方法的建立以及临床常用试剂盒的比对研究 被引量:5
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作者 张雅君 张捷 《首都医科大学学报》 CAS 北大核心 2011年第2期276-282,共7页
目的建立血清尿素测定的酶偶联平衡的参考方法(紫外分光光度法),对此方法进行性能验证,并评价4种临床常用的试剂盒是否可以接受。方法以美国CDC推荐的血清尿素酶偶联平衡候选参考方法为依据建立本实验的参考方法;通过测定标准物质(SRM90... 目的建立血清尿素测定的酶偶联平衡的参考方法(紫外分光光度法),对此方法进行性能验证,并评价4种临床常用的试剂盒是否可以接受。方法以美国CDC推荐的血清尿素酶偶联平衡候选参考方法为依据建立本实验的参考方法;通过测定标准物质(SRM909b),以及参加RELA-2009比对实验(ring-tiral),来验证参考方法的准确性;按CLSI《EP9-A2》要求,以酶偶联平衡参考方法作为比对方法,4种临床常用的检测试剂盒为待评方法,对40份血清样品的结果进行分析比较。结果用酶偶联平衡参考方法测定SRM909b水平1和2,与靶值的偏倚分别为2.19%,0.24%;参加参考实验室国际比对,血清尿素结果在可接受范围内;4种常用的试剂盒在医学决定点(Xc=9.64 mmol/L)预期偏差的95%可信区间值在实验室定义的可接受偏差(4.5%)范围内,也在生物学变异的可接受偏差(5.5%)范围内。结论本室已建立并验证了血清尿素测定的酶偶联平衡参考方法;4种常用血清尿素试剂盒的测定结果与参考方法的测定结果存在差异,但误差在临床可接受范围。 展开更多
关键词 尿素 参考方法 偶联平衡 分光光度法 紫外线 评价研究
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WNK1: analysis of protein kinase structure, downstream targets, and potential roles in hypertension 被引量:4
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作者 Bing-eXU Byung-HoonLEE +5 位作者 XiaoshanMIN LisaLENERTZ CharlesJHEISE SteveSTIPPEC ElizabethJGOLDSMITH MelanieH.COBB 《Cell Research》 SCIE CAS CSCD 2005年第1期6-10,共5页
The WNK kinases are a recently discovered family of serine-threonine kinases that have been shown to play an essential role in the regulation of electrolyte homeostasis. Intronic deletions in the WNK1 gene result in i... The WNK kinases are a recently discovered family of serine-threonine kinases that have been shown to play an essential role in the regulation of electrolyte homeostasis. Intronic deletions in the WNK1 gene result in its overexpression and lead to pseudohypoaldosteronism type II, a disease with salt-sensitive hypertension and hyperkalemia. This review focuses on the recent evidence elucidating the structure of the kinase domain of WNK1 and functions of these kinases in normal and disease physiology. Their functions have implications for understanding the biochemical mechanism that could lead to the retention or insertion of proteins in the plasma membrane. The WNK kinases may be able to influence ion homeostasis through its effects on synaptotagmin function. 展开更多
关键词 KINASE HYPERTENSION SYNAPTOTAGMIN MAP3K MAP4K ERK5 RNAi.
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Fat poetry:a kingdom for PPARγ 被引量:10
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作者 Silvia I Anghel Walter Wahli 《Cell Research》 SCIE CAS CSCD 2007年第6期486-511,共26页
Adipose tissue is not an inert cell mass contributing only to the storage of fat, but a sophisticated ensemble of cellular components with highly specialized and complex functions. In addition to managing the most imp... Adipose tissue is not an inert cell mass contributing only to the storage of fat, but a sophisticated ensemble of cellular components with highly specialized and complex functions. In addition to managing the most important energy reserve of the body, it secretes a multitude of soluble proteins called adipokines, which have beneficial or, alternatively, deleterious effects on the homeostasis of the whole body. The expression of these adipokines is an integrated response to various signals received from many organs, which depends heavily on the integrity and physiological status of the adipose tissue. One of the main regulators of gene expression in fat is the transcription factor peroxisome proliferatoractivated receptor γ (PPARγ), which is a fatty acid- and eicosanoid-dependent nuclear receptor that plays key roles in the development and maintenance of the adipose tissue. Furthermore, synthetic PPAR7 agonists are therapeutic agents used in the treatment of type 2 diabetes. This review discusses recent knowledge on the link between fat physiology and metabolic diseases, and the roles of PPARγ in this interplay via the regulation of lipid and glucose metabolism. Finally, we assess the putative benefits of targeting this nuclear receptor with still-to-be-identified highly selective PPARγ modulators. 展开更多
关键词 adipose tissue energy homeostasis OBESITY peroxisome proliferator-activated receptor
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Transcriptional Analysis of 9-Cis-Epoxycarotenoid Dioxygenase, Glucosyltransferase, 8'-Hydroxylase and B-Glucosidase Genes that Regulate Abscisic Acid Homeostasis around the Onset of Grape Berry Ripening
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作者 Yinghang Dong Jiaxuan Guo 《Journal of Agricultural Science and Technology(A)》 2012年第7期873-881,共9页
The mechanisms for fine-tuning ABA level related to grape berry ripening remain elusive. Here, transcription analysis showed that the mRNA expression level of 9-cis-epoxycarotenoid dioxygenase gene (VvNCED1) increas... The mechanisms for fine-tuning ABA level related to grape berry ripening remain elusive. Here, transcription analysis showed that the mRNA expression level of 9-cis-epoxycarotenoid dioxygenase gene (VvNCED1) increases first, rapidly in mesocarp before the onset of grape berry ripening. After VvNCED1 peaks its expression level, ABA content increases rapidly in mesocarp coupled with an increase in both soluble sugar content and pH value. On the onset of berry ripening, VvNCED1 transcripts decline rapidly to its lowest point, then increases slightly. Whereas, the mRNA expression level of B-glucosidase gene VvBGI, on the whole, increases constantly during grape berry ripening. During berry de-greening, ABA glucosyltransferase (VvGT) and ABA 8'-hydroxylase (VvCYPI) equilibrate ABA level; during berry coloring-up, VvGT predominantly equilibrates ABA level, namely, the up-regulation of ABA level mainly leads from VvNCED1 and VvBG1 gene high expression; the down-regulation of ABA level leads mainly from VvCYP! transcript level both in ABA content- and developmental phase-dependence manner. In conclusion, our main results show that VvNCED1 and VvBG1 genes are closely related to grape berry ripening. 展开更多
关键词 Grape berry ripening ABA metabolism and biosynthesis 9-cis-epoxycarotenoid dioxygenase (NCED) B-glucosidase(BG) ABA glucosyltransferase (GT) ABA 8'-hydroxylase (CYP).
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Orexin-A stimulates the glucose output of porcine hepatocytes 被引量:1
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作者 BIN Yan-fang SHU Gang ZHOU Li-hua CHEN Li-long ZHU Xiao-tong GAO Ping ZHANG Yong-liang ZHANG Shou-quan JIANG Qing-yan 《Journal of Agricultural Science and Technology》 2009年第8期8-13,30,共7页
Orexin-A (OxA) is a key neuropeptide involved in the central control of appetite and the maintenance of energy homeostasis, as well as in the regulation of glycemia. In the present study, OxA receptors, OXtR and OX2... Orexin-A (OxA) is a key neuropeptide involved in the central control of appetite and the maintenance of energy homeostasis, as well as in the regulation of glycemia. In the present study, OxA receptors, OXtR and OX2R were identified to be highly expressed in porcine hepatocytes. 100 nM OxA could rapidly stimulate the glucose output of porcine hepatocytes in 10min. Primary hepatocytes treated by 1 nM-500 nM OxA for 24 h significantly increase the released glucose and the concentration of albumin, total bile acids and triglyceride in the supernatant. The mRNA expression level of such gluconeogenesis and fat mobilization related genes as acetyl-coA carboxylase, glycogen phosphorylase, fatty acid translocase and phosphoenolpyrurate carboxykinase were also up-regulated accordingly. The results first demonstrated that OxA had direct effect on the hepatic glucose and lipid metabolism, which was an aspect for OxA to exert its maintenance function of energy homeostasis. 展开更多
关键词 OXA PORCINE HEPATOCYTES glucose output
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治疗心脑血管病的藏传血宝之机理探讨
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作者 徐秋建 王奇君 《中国保健营养(临床医学学刊)》 2008年第10期78-79,共2页
“藏传血宝”是按照藏医学的核心学说——三因平衡理论配制。配方是火花红景天、藏红花及藏黄芪等,大花红景天,被誉为“九死还魂草”,对心脑血管有超强保护作用。它含有一种具有超强融化能力的物质,称之“平衡融化酶”,它能化血脂... “藏传血宝”是按照藏医学的核心学说——三因平衡理论配制。配方是火花红景天、藏红花及藏黄芪等,大花红景天,被誉为“九死还魂草”,对心脑血管有超强保护作用。它含有一种具有超强融化能力的物质,称之“平衡融化酶”,它能化血脂,化血栓,化复合自由基等。它对甘油三脂、胆固醇、低密度脂蛋白等有极强的溶解作用,而最难解决的复合自由基也同样被分解,这就保证了心脑动脉血管可以恢复畅通,人体恢复健康。 展开更多
关键词 高血压 冠心病 脑梗塞 大花红景天 藏红花 景天红花胶囊 平衡融化
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