采用麦麸固态发酵产蜡蚧轮枝菌菌株(Verticillium lecanii CBS 102071)的分生孢子作为标准方法,对甘蔗渣和聚胺酯泡沫(PU)吸附液体培养基固态发酵产蜡蚧轮枝菌的分生孢子的方法进行评价,并对其三者孢子的几丁质酶活性和蛋白酶活性进行测...采用麦麸固态发酵产蜡蚧轮枝菌菌株(Verticillium lecanii CBS 102071)的分生孢子作为标准方法,对甘蔗渣和聚胺酯泡沫(PU)吸附液体培养基固态发酵产蜡蚧轮枝菌的分生孢子的方法进行评价,并对其三者孢子的几丁质酶活性和蛋白酶活性进行测试,结果3种方法所产孢子几丁质酶活性和蛋白酶活性较为接近,并最终确立了孢子最佳采收时间。再对PU软质泡沫法产生的蜡蚧轮枝菌分生孢子进行抗逆性测试,发现其抗逆环境性能良好:在高温30、33、35、40℃下,分生孢子也能萌发,萌发率依次约为97%、85%、32%、10%;在相对湿度90%、85%、81%、75%、71%、65%下,分生孢子萌发率依次约为98%、96%、83%、15%、5%、0%;用紫外线辐射30～300 s时,分生孢子的萌发率从96%降到24%。展开更多
[Objective] To investigate the optimal enzyme for the hydrolysis of corn gluten meal and the optimal hydrolysis conditions for the enzyme. [Method] Nine kinds of enzymes were used to hydrolyze the corn gluten meal, us...[Objective] To investigate the optimal enzyme for the hydrolysis of corn gluten meal and the optimal hydrolysis conditions for the enzyme. [Method] Nine kinds of enzymes were used to hydrolyze the corn gluten meal, using the formaldehyde titration method for the determination of hydrolysis degree, and orthogonal test was used to determine the optimal hydrolysis conditions for double enzymes hydrol- ysis of corn gluten meal. [Result] The optimal pretreatment condition for corn gluten meal is heating at 121 ~C for 30 min. The double enzyme hydrolysis for the pro- treated corn gluten meal using 2709 alkaline protease and flavourzyme showed that the degree of hydrolysis could reach 32.4% with enzyme addition amount of 4%, hy- drolysis time of 4 h at 45℃ and pH=7.0. [Conclusion] This study laid the foundation for the study on the preparation of bioactive peptides such as oligopeptide with high F value and antihypertensive peptides, further improving the corn intensive process- ing industrial chain.展开更多
文摘采用麦麸固态发酵产蜡蚧轮枝菌菌株(Verticillium lecanii CBS 102071)的分生孢子作为标准方法,对甘蔗渣和聚胺酯泡沫(PU)吸附液体培养基固态发酵产蜡蚧轮枝菌的分生孢子的方法进行评价,并对其三者孢子的几丁质酶活性和蛋白酶活性进行测试,结果3种方法所产孢子几丁质酶活性和蛋白酶活性较为接近,并最终确立了孢子最佳采收时间。再对PU软质泡沫法产生的蜡蚧轮枝菌分生孢子进行抗逆性测试,发现其抗逆环境性能良好:在高温30、33、35、40℃下,分生孢子也能萌发,萌发率依次约为97%、85%、32%、10%;在相对湿度90%、85%、81%、75%、71%、65%下,分生孢子萌发率依次约为98%、96%、83%、15%、5%、0%;用紫外线辐射30～300 s时,分生孢子的萌发率从96%降到24%。
文摘[Objective] To investigate the optimal enzyme for the hydrolysis of corn gluten meal and the optimal hydrolysis conditions for the enzyme. [Method] Nine kinds of enzymes were used to hydrolyze the corn gluten meal, using the formaldehyde titration method for the determination of hydrolysis degree, and orthogonal test was used to determine the optimal hydrolysis conditions for double enzymes hydrol- ysis of corn gluten meal. [Result] The optimal pretreatment condition for corn gluten meal is heating at 121 ~C for 30 min. The double enzyme hydrolysis for the pro- treated corn gluten meal using 2709 alkaline protease and flavourzyme showed that the degree of hydrolysis could reach 32.4% with enzyme addition amount of 4%, hy- drolysis time of 4 h at 45℃ and pH=7.0. [Conclusion] This study laid the foundation for the study on the preparation of bioactive peptides such as oligopeptide with high F value and antihypertensive peptides, further improving the corn intensive process- ing industrial chain.
