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碱性纤维素酶产生菌的筛选及产酶条件的研究 被引量:3
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作者 陈亚平 王白娟 +2 位作者 刘鲁峰 彭谦 赖泳红 《安徽农业科学》 CAS 北大核心 2010年第17期8844-8846,共3页
[目的]筛选碱性纤维素酶产生菌,并优化其产酶条件。[方法]对从土壤样品中分离到的100余株菌进行平板筛选,获得1株产碱性纤维素酶的菌株ZJJ-1,并对其进行液体培养基成分及发酵产酶条件优化。[结果]培养基最佳配方为:麸皮0.5%,大豆粉2%,KH... [目的]筛选碱性纤维素酶产生菌,并优化其产酶条件。[方法]对从土壤样品中分离到的100余株菌进行平板筛选,获得1株产碱性纤维素酶的菌株ZJJ-1,并对其进行液体培养基成分及发酵产酶条件优化。[结果]培养基最佳配方为:麸皮0.5%,大豆粉2%,KH2PO40.2%,NaCl 0.7%;最优产酶条件为:37℃、175 r/min培养48 h,初始pH值为7。在此条件下,最高酶活水平达51.20 U/ml。[结论]为碱性纤维素酶的后续研究提供了优良的菌种资源。 展开更多
关键词 筛选 碱性纤维素 培养条件 酶活水平
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Changes in Growth, Carbon and Nitrogen Enzyme Activity and mRNA Accumulation in the Halophilic Microalga Dunaliella viridis in Response to NaCl Stress 被引量:1
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作者 WANG Dongmei WANG Weiwei +1 位作者 XU Nianjun SUN Xue 《Journal of Ocean University of China》 SCIE CAS 2016年第6期1094-1100,共7页
Abstract Many species of microalga Dunaliella exhibit a remarkable tolerance to salinity and are therefore ideal for probing the effects of salinity. In this work, we assessed the effects of NaC1 stress on the growth,... Abstract Many species of microalga Dunaliella exhibit a remarkable tolerance to salinity and are therefore ideal for probing the effects of salinity. In this work, we assessed the effects of NaC1 stress on the growth, activity and mRNA level of carbon and nitrogen metabolism enzymes of D. viridis. The alga could grow over a salinity range of 0.44 mol L-t to 3.00 mol L-1 NaCI, but the most rapid growth was observed at 1.00molL-1NaC1, followed by 2.00 molL-l NaC1. Paralleling these growth patterns, the highest initial and total Rubisco activities were detected in the presence of 1.00molL-t NaC1, decreasing to 37.33% and 26.39% of those values, re- spectively, in the presence of 3.00 mol L-1 NaC1, respectively. However, the highest extracellular carbonic anhydrase (CA) activity was measured in the presence of 2.00molL-1 NaC1, followed by 1.00molL-1NaC1. Different from the two carbon enzymes, nitrate reductase (NR) activity showed a slight change under different NaC1 concentrations. At the transcriptional level, the mRNAs of Rubisco large subunit (rbcL), and small subunit (rbcS), attained their highest abundances in the presence of 1.00 and 2.00molL-1 NaC1, respectively. The CA mRNA accumulation was induced from 0.44molL ~ to 3.00molL-1 NaC1, but the NR mRNA showed the decreasing tendency with the increasing salinity. In conclusion, the growth and carbon fixation enzyme of Rubisco displayed similar tendency in response to NaC1 stress, CA was proved be salt-inducible within a certain salinity range and NR showed the least effect by NaC1 in D. viridis. 展开更多
关键词 Dunaliella viridis NaC1 RUBISCO carbonic anhydrase nitrate reductase
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Effects of elevated CO_2 on the photosynthesis and nitrate reductase activity of Pyropia haitanensis(Bangiales,Rhodophyta) grown at different nutrient levels 被引量:2
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作者 刘春香 邹定辉 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2015年第2期419-429,共11页
Pyropia haitanensis, a commercially important species, was cultured at two CO2 concentrations (390× 10^-6 and 700× 10^-6 (parts per million)) and at low and high nutrient levels, to explore the effect of... Pyropia haitanensis, a commercially important species, was cultured at two CO2 concentrations (390× 10^-6 and 700× 10^-6 (parts per million)) and at low and high nutrient levels, to explore the effect of elevated CO2 on the species under nutrient enrichment. Results show that in CO2-enriched thalli, relative growth rate (RGR) was enhanced under nutrient enrichment. Elevated CO2 decreased phycobiliprotein (PB) contents, but increased the contents of soluble carbohydrates. Nutrient enrichment increased the contents of chlorophyll a (Chl a) and PB, while soluble carbohydrate content decreased. CO2 enrichment enhanced the relative maximum electronic transport rate and light saturation point. In nutrient-enriched thalli the activity of nitrate reductase (NRA) increased under elevated CO2. An instantaneous pH change in seawater (from 8.1 to 9.6) resulted in reduction of NRA, and the thalli grown under both elevated CO2 and nutrient enrichment exhibited less pronounced reduction than in algae grown at the ambient CO2. The thermal optima of NRA under elevated CO2 and/or nutrient enrichment shifted to a lower temperature (10-15 ℃) compared to that in ambient conditions (20℃). We propose that accelerated photosynthesis could result in growth increment. N assimilation remained high in acidified seawater and reflected increased temperature sensitivity in response to elevated CO_2 and eutrophication. 展开更多
关键词 Pyropia haitanensis PHOTOSYNTHESIS nitrate assimilation elevated CO2 EUTROPHICATION
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The Expression of Ca N and CaMK is Associated with Lipogenesis in the Muscle of Chicken
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作者 Ye YANG Jiao SONG +2 位作者 Ruiqi FUI Yanfa SUN Jie WEN 《Agricultural Science & Technology》 CAS 2015年第4期761-764,共4页
Intramuscular fat (IMF) content in chickens significantly contributes to meat quality. The main objective of this study was to assess the expression of calcineudn (CAN) and Ca^2+/calmodutin-dependent protein kina... Intramuscular fat (IMF) content in chickens significantly contributes to meat quality. The main objective of this study was to assess the expression of calcineudn (CAN) and Ca^2+/calmodutin-dependent protein kinase (CAME) in lipogene- sis in chicken muscle. The chickens were slaughtered and sampled at the ages of 4, 8, and 16 weeks, respectively. IMF content and the expression of CaN subunits and CaMK isoforms were measured in thigh muscle tissue. The results showed that the IMF contents were higher in chickens at the age of 16 weeks compared with those in chickens at the ages of 4 and 8 weeks (P〈0.05). The expression levels of fatty acid synthase (FAS) and fatty acid translocase CD36 (FAT/CD36) mRNA in 16-week-old chickens were all significantly up-regulated compared with those in 4-week-old chickens (P〈0.05). The mRNA levels of CaNB and CaMK IV in 16-week-old chickens were significantly lower than those in 4-week-old chickens (P〈0.05). But the CaMK II mRNA levels in 16-week-old chickens were significantly higher than those in 4-week-old chickens (P〈0.05). To investigate the roles of CaMK and CaN in adipogenesis, SV cells were incubated in standard adipogenesis medium for 24 h and treated with specific inhibitor of CaMK and CaN. The ex- pressions of CCAAT/enhancer binding protein β(C/EBPJ3), sterol regulatory element- binding protein 1 (SREBP1) and peroxisome proliferation-activated receptor ), (PPARy) were dramatically enhanced by CsA and CaN inhibitor (P〈0.05). KN93, a CaMK Ⅱ inhibitor, dramatically repressed the expression of those lipogenic genes (P〈0.05). All the results above indicated that CaN and CaMK had different effects on adipogenesis in the muscle of chickens. 展开更多
关键词 Ca^2+/calmodulin-dependent protein kinase CALCINEURIN CHICKEN Regula-tion
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