PVA/Chitosan(CS) composite membrane was used for enzyme processing of fats and oils. The concentration of lipase and cross-linking agent which influence the immobilization of lipase in membrane were determined. Epichl...PVA/Chitosan(CS) composite membrane was used for enzyme processing of fats and oils. The concentration of lipase and cross-linking agent which influence the immobilization of lipase in membrane were determined. Epichlorohydrin is used as the cross-linking agent. The immobilized lipase is 0.66 u·cm -2 and the recovery of immobilized lipase is 24%. The membrane reactor was tested to synthesis monoglyceride(MG), which could be used many times without loss conversion yield of MG. The PVA/CS lipase membrane reactor is a new reactor for lipase catalytic biphase systems.展开更多
A multiphase enzyme membrane reactor using aqueous organic biphase instead of water phase alone as the reaction medium was employed to investigate the lipase catalyzed synthesis of bioactive dipeptides. The medium eff...A multiphase enzyme membrane reactor using aqueous organic biphase instead of water phase alone as the reaction medium was employed to investigate the lipase catalyzed synthesis of bioactive dipeptides. The medium effect on dipeptide yield was first studied. When N acetyl L phenylalanine ethyl ester(APEE) was used as a carboxyl component, the reactivity order of amino acid amides was found to be L Leu NH 2> L Val NH 2> L Ala NH 2> L Gly NH 2. The didpetide, N Ac L Phe L Leu NH 2, could be synthesized in the multiphase enzyme membrane reactor in a high yield and purity due to the simultaneous separation and reaction.展开更多
基金国家自然科学基金! (No .2 96760 0 1 )山东大学国家微生物技术重点实验室开放基金河北省自然科学基金资助
文摘PVA/Chitosan(CS) composite membrane was used for enzyme processing of fats and oils. The concentration of lipase and cross-linking agent which influence the immobilization of lipase in membrane were determined. Epichlorohydrin is used as the cross-linking agent. The immobilized lipase is 0.66 u·cm -2 and the recovery of immobilized lipase is 24%. The membrane reactor was tested to synthesis monoglyceride(MG), which could be used many times without loss conversion yield of MG. The PVA/CS lipase membrane reactor is a new reactor for lipase catalytic biphase systems.
文摘A multiphase enzyme membrane reactor using aqueous organic biphase instead of water phase alone as the reaction medium was employed to investigate the lipase catalyzed synthesis of bioactive dipeptides. The medium effect on dipeptide yield was first studied. When N acetyl L phenylalanine ethyl ester(APEE) was used as a carboxyl component, the reactivity order of amino acid amides was found to be L Leu NH 2> L Val NH 2> L Ala NH 2> L Gly NH 2. The didpetide, N Ac L Phe L Leu NH 2, could be synthesized in the multiphase enzyme membrane reactor in a high yield and purity due to the simultaneous separation and reaction.
