[Objective] This study aimed to isolate an acidophilic fungus and analyze the acidophilic enzymes secreted by this fungus. [Method] A heterotrophic fungus was isolated from the leaching solution of a uranium ore in Ji...[Objective] This study aimed to isolate an acidophilic fungus and analyze the acidophilic enzymes secreted by this fungus. [Method] A heterotrophic fungus was isolated from the leaching solution of a uranium ore in Jiangxi Province using oligotrophic acid selective medium （pH 2.5）, and was named RBS-6. This strain was then identified according to its colony morphology and molecular indicator rDNA-ITS. Finally, the glycoside hydrolases secreted by RBS-6 were analyzed. [Result] This fungus RBS-6 was acidophilic, and grew best at pH4.0. Its rDNA-ITS sequence shared the highest homology （98%） with that of Phialophora sp. CGMCC 3329 （GU 082377）. So it was identified as a fungus of Phialophora sp., and was temporarily named as Phialophora sp. RBS-6. It can produce six glycoside hydrolases, in cluding α-galactosidase glucosidase, β-glucosidase, β-mannanase and β-glucanase. All the enzymes were acidophilic, for which the optimum reaction pH was 3.0-4.0. Among them, β-glucanase exhibited the highest activity at pH 3.5 and 50 ℃; in addition, it was heat-stable as 58% of the enzyme activity was remained after incubation at 50 ℃ for 60 min. [Conclusion] The isolated fungus which was identified as an acidophilic member of Phialophora sp., was a new strain producing acidophilic enzymes. This study supplied new data for the research on Phialophora fungi.展开更多
The cannabinoid （CB） receptors, endocannabinoids （eCB） and their synthesizing and catabolizing enzymes and the proteins involved in their transport, constitute what is now recognized as the eCB system. The eCBs ar...The cannabinoid （CB） receptors, endocannabinoids （eCB） and their synthesizing and catabolizing enzymes and the proteins involved in their transport, constitute what is now recognized as the eCB system. The eCBs are a class of lipids that have been identifed as retro-grade messengers and produce their effects via presyn-aptic CB receptors. The major function of the eCBs has been suggested to be that of modulating the release of several neurotransmitters implicated in a number of biological functions that include reward and reinforce-ment. There is now significant evidence to suggest that the eCB system plays an important role in the development of alcohol tolerance, dependence and relapse. Recent studies suggest that the pharmacological manipulation of the eCB system has the potential not only to block the direct reinforcing properties of alcohol but also alleviate behavioral abnormalities associated with relapse. There is also accumulating evidence that points to the possible utility of the eCB system targeted drugs in the treatment of alcoholism-related behavioral disorders. The agents that block CB1 receptor function or inhibit the synthesis of eCBs are attractive candidate drugs that need to be explored. Further understanding of the role of the eCB system in molecular mechanism/s that underlies alcoholism-related behaviors should lead to a better treatment of this devastating disorder.展开更多
The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, espe- cially the hormone-related differences in maturing male fish has seldom been documented. In this ...The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, espe- cially the hormone-related differences in maturing male fish has seldom been documented. In this study, a full length cDNA sequence of the vasa gene was cloned from Japanese sea bass, Lateolabraxjaponicas, and it was namedjsb-vasa. Homology analysis showed thatjsb-vasa was closely related to its teleost homologs. The spatial distribution ofjsb-vasa indicated that it was only highly ex- pressed in testis, showing its germ cell-specific expression pattern. During the testicular development cycle, jsb-vasa was highly expressed during early period of spermatogenesis, and reduced when spermatogenesis advanced. In addition, the jsb-vasa gene ex- pression was significantly inhibited at 6 h, 12 h and 24 h after injecting hCG （human ehorionic gonadotropin） and GnRHa （Gonad- otropin-releasing hormone analogue）, indicating thatjsb-vasa gene may play an important role in spermatogenesis of Japanese sea bass, and be under the regulation of external sex hormones.展开更多
基金Supported by Manufacture - Learning - Research Cooperation Project of Education Department of Jiangxi Province(GJJ09008)Nuclear Power Development Projects [COSTIND, (2009)1230]~~
文摘[Objective] This study aimed to isolate an acidophilic fungus and analyze the acidophilic enzymes secreted by this fungus. [Method] A heterotrophic fungus was isolated from the leaching solution of a uranium ore in Jiangxi Province using oligotrophic acid selective medium （pH 2.5）, and was named RBS-6. This strain was then identified according to its colony morphology and molecular indicator rDNA-ITS. Finally, the glycoside hydrolases secreted by RBS-6 were analyzed. [Result] This fungus RBS-6 was acidophilic, and grew best at pH4.0. Its rDNA-ITS sequence shared the highest homology （98%） with that of Phialophora sp. CGMCC 3329 （GU 082377）. So it was identified as a fungus of Phialophora sp., and was temporarily named as Phialophora sp. RBS-6. It can produce six glycoside hydrolases, in cluding α-galactosidase glucosidase, β-glucosidase, β-mannanase and β-glucanase. All the enzymes were acidophilic, for which the optimum reaction pH was 3.0-4.0. Among them, β-glucanase exhibited the highest activity at pH 3.5 and 50 ℃; in addition, it was heat-stable as 58% of the enzyme activity was remained after incubation at 50 ℃ for 60 min. [Conclusion] The isolated fungus which was identified as an acidophilic member of Phialophora sp., was a new strain producing acidophilic enzymes. This study supplied new data for the research on Phialophora fungi.
基金Supported by Funds from the National Institute of Health,Bethesda,United StatesAmerican Foundation for SuicidePrevention
文摘The cannabinoid （CB） receptors, endocannabinoids （eCB） and their synthesizing and catabolizing enzymes and the proteins involved in their transport, constitute what is now recognized as the eCB system. The eCBs are a class of lipids that have been identifed as retro-grade messengers and produce their effects via presyn-aptic CB receptors. The major function of the eCBs has been suggested to be that of modulating the release of several neurotransmitters implicated in a number of biological functions that include reward and reinforce-ment. There is now significant evidence to suggest that the eCB system plays an important role in the development of alcohol tolerance, dependence and relapse. Recent studies suggest that the pharmacological manipulation of the eCB system has the potential not only to block the direct reinforcing properties of alcohol but also alleviate behavioral abnormalities associated with relapse. There is also accumulating evidence that points to the possible utility of the eCB system targeted drugs in the treatment of alcoholism-related behavioral disorders. The agents that block CB1 receptor function or inhibit the synthesis of eCBs are attractive candidate drugs that need to be explored. Further understanding of the role of the eCB system in molecular mechanism/s that underlies alcoholism-related behaviors should lead to a better treatment of this devastating disorder.
基金supported by the National Key Technologies R&D Program of China(2011BAD13B03)
文摘The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, espe- cially the hormone-related differences in maturing male fish has seldom been documented. In this study, a full length cDNA sequence of the vasa gene was cloned from Japanese sea bass, Lateolabraxjaponicas, and it was namedjsb-vasa. Homology analysis showed thatjsb-vasa was closely related to its teleost homologs. The spatial distribution ofjsb-vasa indicated that it was only highly ex- pressed in testis, showing its germ cell-specific expression pattern. During the testicular development cycle, jsb-vasa was highly expressed during early period of spermatogenesis, and reduced when spermatogenesis advanced. In addition, the jsb-vasa gene ex- pression was significantly inhibited at 6 h, 12 h and 24 h after injecting hCG （human ehorionic gonadotropin） and GnRHa （Gonad- otropin-releasing hormone analogue）, indicating thatjsb-vasa gene may play an important role in spermatogenesis of Japanese sea bass, and be under the regulation of external sex hormones.
