Five preparation methods were used to purify the crude polysaccharide which was the co\|products of preparation saponin from \%Ophiopogonis japonicus \%(Thunb.) Ker\|Gawl. The results indicate that the method of enzym...Five preparation methods were used to purify the crude polysaccharide which was the co\|products of preparation saponin from \%Ophiopogonis japonicus \%(Thunb.) Ker\|Gawl. The results indicate that the method of enzyme hydrolysis could purify the crude polysaccharide efficiently. The content of polysaccharide is more than 85% and could be used as the foods and medicine raw material. Advanced test showed that the polysaccharide is mainly consist of glucose, fructose, xylose,rhamnose and galactose.展开更多
Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain p...Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain protein hydrolysates with high ni-trogen recovery and low bitter taste,enzymatic hydrolysis was performed using eight commercially available proteases.It turned out that the optimum enzyme was the ‘Mixed enzymes for animal proteolysis’.An enzyme dosage of 4%,a temperature of 50℃,and a hydrolysis time of 300 min were found to be the optimum conditions to obtain high NR (84.28%) and degree of hydrolysis (DH,16.18%) by orthogonal experiments.Glutamic acid was the most abundant amino acid of MDP (defatted mackerel protein) and MDPH (defatted mackerel protein hydrolysates).Compared with the FAO/WHO reference protein,the essential amino acid chemical scores (CS) were greater than 1.0 (1.0 1.7) in MDPH,which is reflective of high nutritional value.This,coupled with the light color and slight fishy odor,indicates that MDPH would potentially have a wide range of applications such as nutritional additives,functional ingredients,and so on.展开更多
文摘Five preparation methods were used to purify the crude polysaccharide which was the co\|products of preparation saponin from \%Ophiopogonis japonicus \%(Thunb.) Ker\|Gawl. The results indicate that the method of enzyme hydrolysis could purify the crude polysaccharide efficiently. The content of polysaccharide is more than 85% and could be used as the foods and medicine raw material. Advanced test showed that the polysaccharide is mainly consist of glucose, fructose, xylose,rhamnose and galactose.
基金supported by the National Natural Science Foundation of China (No.30871943)the National Natural Science Foundation of China (No.3080 0858)
文摘Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain protein hydrolysates with high ni-trogen recovery and low bitter taste,enzymatic hydrolysis was performed using eight commercially available proteases.It turned out that the optimum enzyme was the ‘Mixed enzymes for animal proteolysis’.An enzyme dosage of 4%,a temperature of 50℃,and a hydrolysis time of 300 min were found to be the optimum conditions to obtain high NR (84.28%) and degree of hydrolysis (DH,16.18%) by orthogonal experiments.Glutamic acid was the most abundant amino acid of MDP (defatted mackerel protein) and MDPH (defatted mackerel protein hydrolysates).Compared with the FAO/WHO reference protein,the essential amino acid chemical scores (CS) were greater than 1.0 (1.0 1.7) in MDPH,which is reflective of high nutritional value.This,coupled with the light color and slight fishy odor,indicates that MDPH would potentially have a wide range of applications such as nutritional additives,functional ingredients,and so on.
