AdoMet plays numerous roles of being the major methyl-group donor in trans-methylation reactions. To gain insight into the possible functions of the AdoMet protein of Suaeda salsa L. in response to salt stress, S aden...AdoMet plays numerous roles of being the major methyl-group donor in trans-methylation reactions. To gain insight into the possible functions of the AdoMet protein of Suaeda salsa L. in response to salt stress, S adenosylmethionine synthetase gene (SAMS2) was analyzed. We isolated SAMS2 cDNA clone (AF321001) from a lambda -Zap cDNA library constructed from the halophyte S. salsa Pall aerial tissue treated with 400 mmol/L NaCl. SsSAMS2 was found to encode a S-adenolyl-L-methionine synthetase enzyme (AdoMet synthetase). The fragment was 1 531 bp with an open reading frame of 395 amino acids, the calculated molecular weight was about 43 kD. SsSAMS2 showed the highest homology to SAMS2 gene of Catharanthus roseus G. Don., with 93% identity in deduced amino acid sequence. Southern blotting analysis showed that SsSAMS2 might be a two-copy gene in S. salsa genome. Northern blot indicated that the cDNA was up-regulated by salt and other stresses. Enzyme activity assay indicated that the activity of SAMS2 increased under NaCl stress.展开更多
The wettability,surfactivity and the correlation between wettability and surfactivity of sodium diethylhexylphosphate,sodium diethylhexyl polyoxyethylene phosphate and their complex in NaOH solutions were studied.A co...The wettability,surfactivity and the correlation between wettability and surfactivity of sodium diethylhexylphosphate,sodium diethylhexyl polyoxyethylene phosphate and their complex in NaOH solutions were studied.A complex alkali resistant phosphate surfactant with good permeability was prepared.The wettability of surfactants was investigated by measuring the immersion time,sinking time and capillary effects of nature cotton grey fabric in NaOH solutions.The surfactivity of the surfactants was characterized by measuring the surface tension.The effect of the complex on the surface appearance of cotton grey fabric was also investigated with a scanning electron microscope(SEM) .The results show that all the surfactants exhibit good wettability for cotton grey fabric in 0.5-5.0 mol/L of NaOH solutions,the complex system exhibits better wettability in 5.0-7.0 mol/L of NaOH solutions,in comparison with either corresponding single surfactant component employed,and wettability is well correlative with the surfactivities of the surfactant.SEM images indicate that the cotton grey fabric is well wetted by the alkaline surfactant solution and the quality of fabric is improved.展开更多
文摘AdoMet plays numerous roles of being the major methyl-group donor in trans-methylation reactions. To gain insight into the possible functions of the AdoMet protein of Suaeda salsa L. in response to salt stress, S adenosylmethionine synthetase gene (SAMS2) was analyzed. We isolated SAMS2 cDNA clone (AF321001) from a lambda -Zap cDNA library constructed from the halophyte S. salsa Pall aerial tissue treated with 400 mmol/L NaCl. SsSAMS2 was found to encode a S-adenolyl-L-methionine synthetase enzyme (AdoMet synthetase). The fragment was 1 531 bp with an open reading frame of 395 amino acids, the calculated molecular weight was about 43 kD. SsSAMS2 showed the highest homology to SAMS2 gene of Catharanthus roseus G. Don., with 93% identity in deduced amino acid sequence. Southern blotting analysis showed that SsSAMS2 might be a two-copy gene in S. salsa genome. Northern blot indicated that the cDNA was up-regulated by salt and other stresses. Enzyme activity assay indicated that the activity of SAMS2 increased under NaCl stress.
基金Project(20573079) supported by the National Natural Science Foundation of China
文摘The wettability,surfactivity and the correlation between wettability and surfactivity of sodium diethylhexylphosphate,sodium diethylhexyl polyoxyethylene phosphate and their complex in NaOH solutions were studied.A complex alkali resistant phosphate surfactant with good permeability was prepared.The wettability of surfactants was investigated by measuring the immersion time,sinking time and capillary effects of nature cotton grey fabric in NaOH solutions.The surfactivity of the surfactants was characterized by measuring the surface tension.The effect of the complex on the surface appearance of cotton grey fabric was also investigated with a scanning electron microscope(SEM) .The results show that all the surfactants exhibit good wettability for cotton grey fabric in 0.5-5.0 mol/L of NaOH solutions,the complex system exhibits better wettability in 5.0-7.0 mol/L of NaOH solutions,in comparison with either corresponding single surfactant component employed,and wettability is well correlative with the surfactivities of the surfactant.SEM images indicate that the cotton grey fabric is well wetted by the alkaline surfactant solution and the quality of fabric is improved.
