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甘蓝型油菜籽粒油酸、亚油酸、亚麻酸和蛋白质含量变异及相关性分析 被引量:9
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作者 姚琳 孙璇 +2 位作者 咸拴狮 耿振刚 杜春芳 《中国粮油学报》 CAS CSCD 北大核心 2021年第5期82-87,共6页
对现有种质资源进行分析,有利于甘蓝型油菜品质育种亲本选配及种质创新。本研究对312份甘蓝型油菜种质资源进行近红外光谱检测,通过相关性分析和聚类分析探究油菜籽粒中油酸、亚油酸、亚麻酸和蛋白质含量及它们间的相关性。结果表明:油... 对现有种质资源进行分析,有利于甘蓝型油菜品质育种亲本选配及种质创新。本研究对312份甘蓝型油菜种质资源进行近红外光谱检测,通过相关性分析和聚类分析探究油菜籽粒中油酸、亚油酸、亚麻酸和蛋白质含量及它们间的相关性。结果表明:油酸含量与亚油酸及蛋白质含量呈极显著负相关,亚油酸含量与蛋白质含量呈极显著正相关;在阈值为2时,可将312份种质资源划分为6个类群,其中第Ⅱ类群适于选育高油酸亲本,第Ⅲ类群适于选育高亚麻酸亲本,第Ⅳ、Ⅵ类群适于选育高亚油酸、高蛋白亲本。 展开更多
关键词 甘蓝型 酸亚油 蛋白质 相关性
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Preparation of High-Purity Linolenic Acid from Oil of Lithospermum Erythrorhizon by Urea Inclusion and Column Chromatography 被引量:9
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作者 韩喜江 徐平 +3 位作者 孟祥丽 梁志华 张宗双 杨占成 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第1期53-57,共5页
Aim To separate high purity linolenic acid from the oil of Lithospermumerythrorhizon growing in the Northeast of China. Methods Urea inclusion and column chromatographywere used. Results Unsaturated fatty acid was sep... Aim To separate high purity linolenic acid from the oil of Lithospermumerythrorhizon growing in the Northeast of China. Methods Urea inclusion and column chromatographywere used. Results Unsaturated fatty acid was separated, with a purity of 99.30 wt% of linolenicacid. Conclusion The experiment shows excellent reproducibility and high feasibility for industrialproduction. 展开更多
关键词 ltihospermum erythrorhizon linolenic acid urea inclusion columnchromatography
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A PRELIMINARY STUDY ON THE INTRODUCTION OF DESCURAINIA SOPHIA, AN OIL PLANT SPECIES FOR INDUSTRIAL USES 被引量:5
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作者 罗鹏 杨毅 +1 位作者 高福利 蓝泽蘧 《Acta Botanica Sinica》 CSCD 1997年第5期477-479,共3页
The authors had collected some cruciferous oil plants with high linolenic acid content in Sichuan Province. The result of fatty acid analysis had shown that Malcolmia africrma (L.) R. Br. and Arabis hirsuta (L.) Scop,... The authors had collected some cruciferous oil plants with high linolenic acid content in Sichuan Province. The result of fatty acid analysis had shown that Malcolmia africrma (L.) R. Br. and Arabis hirsuta (L.) Scop, have a higher linolenic acid content of 57.37% and 51.38% respectively . Thus they are worthy for further study. Descurainia sophia (L.) Webb. ex Prantl, especially that collected from Hongyuan County, has a high seed oil content of 44.17% , a linolenic acid content of 40.9 % and a high seed yield of 1 264.5 kg/hm2 . It is suggested that this oil plant species can be introduced for industrial utilization. 展开更多
关键词 Oil plant Linolenic acid Descurainia sophia
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Cloning of Cotton Delta-12 Oleate Desaturase Gene FAD2-1 and Construction of Its ihpRNA and amiRNA Interference Vectors 被引量:1
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作者 赵立群 李红岺 +3 位作者 李仁 李蔚 华金平 郭仰东 《Agricultural Science & Technology》 CAS 2012年第11期2281-2283,2286,共4页
Delta-12 oleate desaturase gene (FAD2-1) which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of cottonseed oil. By employing RT-PCR method, full length cDNA of cott... Delta-12 oleate desaturase gene (FAD2-1) which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of cottonseed oil. By employing RT-PCR method, full length cDNA of cotton delta-12 oleate desat- urase gene GhFAD2-1 containing an open reading frame of 1 158 bp was cloned for constructing RNAi vector. A 515 bp long specific fragment of this gene was se- lected for constructing ihpRNA vector under the control of a seed-specific promoter NAPIN, named pFGC1008-NAPIN-FAD2-1; meanwhile miRNA gene-silencing vector pCAMBIA1302-amiRNA-FAD2-1 targeting GhFAD2-1 was also constructed. 展开更多
关键词 Cotton delta-12 oleate desaturase gene GhFAD2-1 ihpRNA interferencevector amiRNA interference vector High oleic acid contents
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Preparation of Conjugated Linoleic Acid of High Purity and Its Apparent Kinetic Characteristics During Formation 被引量:4
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作者 唐向阳 郭诤 张根旺 《Transactions of Tianjin University》 EI CAS 2002年第1期27-32,共6页
Conjugated linoleic acid (CLA) is a fatty acid with physiological activities and potential application prospect. This paper focuses on the method of synthesis of conjugated linoleic acid of high purity and the process... Conjugated linoleic acid (CLA) is a fatty acid with physiological activities and potential application prospect. This paper focuses on the method of synthesis of conjugated linoleic acid of high purity and the process line and conditions for its purification that can be used in large scale production. CLA of more than 95% purity was prepared by means of urea adduct purification and conjugation using safflower oil as material. The total recovery of the product adds up to more than 48%. The reactive kinetics about linoleic acid from sunflower oil converted into CLA was investigated, and its apparent kinetic model was also established, which can be used as a base for industrial designs. 展开更多
关键词 linoleic acid conjugated linoleic acid PREPARATION apparent kinetics urea adduct alkali catalyzed conjugation safflower oil
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Effect of Altering Linoleic Acid and Linolenic Acid Dietary Ratios on the Performance and Tissue Fatty Acid Profiles of Prawn, Macrobrachium rosenbergii (de Man 1879) Post Larvae 被引量:11
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作者 A. M. A. S. Goda M. M. Habashy +5 位作者 M. A. Hanafy R. A. Mohamed H. A. Mabrouk T. M. El-Afifi S. A.Nada K. A. Mohamed 《Journal of Agricultural Science and Technology(B)》 2011年第7期1032-1044,共13页
A 90-day experiment was conducted to investigate the effects of different dietary linoleic acid (LA; 18:2n-6) and linolenic acid ratios (LNA; 18:3n-3) on growth induces, feed utilization and tissue fatty acid pr... A 90-day experiment was conducted to investigate the effects of different dietary linoleic acid (LA; 18:2n-6) and linolenic acid ratios (LNA; 18:3n-3) on growth induces, feed utilization and tissue fatty acid profile of freshwater prawn, Macrobrachium rosenbergii post-larvae (PL). The experiment was conducted in cubic indoor fiberglass tanks, each holding 700 L in triplicate. Post-larvae with an average weight of 20.8 ± 0.20 mg were stocked at 80 PL m2. Five experimental isocaloric (15.06 MJ kgl digestible energy), and isonitrogenous (30.45% digestible protein) diets were formulated by blending of soybean oil and linseed oil to containing five dietary LA/LNA ratios (7.80, 2.75, 1.28, 0.65 and 0.30). The highest survival values were recorded for prawn PL fed diet containing 0.65 LA/LAN ratios. Growth indices of PL significantly increased (P 〈 0.05) with decreased dietary LA/LAN ratios to 0.65. The same trend was observed for the highest (P ≤ 0.05) protein efficiency ratio, protein productive value, fat retention, energy retention and best feed conversion ratio. The total whole tissue polyunsaturated fatty acid composition of M. rosenbergii PL was dominated by LA followed by LAN. Post larvae fed the diets containing higher LA/LNA ratios showed a higher tissue LA/LNA ratio. The obtained findings revealed that fatty acid patterns ofM. rosenbergii PL were influenced by fatty acid profiles of diets. The diet containing 0.65 LA/LNA ratio is recommended to obtaining optimum growth performance and feed utilization for M. rosenbergii PL. 展开更多
关键词 Fatty acids linoleic linolenic Macrobrachium rosenbergii dietary ratio.
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Effect of cis-9,trans-11-conjugated linoleic acid on cell cycle of gastric adenocarcinoma cell line(SGC-7901) 被引量:26
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作者 Jia-Ren Liu Bai-Xiang Li Bing-Qing Chen Ying-ben Xue Yan-Mei Yang Yu-Mei Zheng,Department of Toxicological Health,Public Health College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Xiao-Hui Han ICU of Cardiological Surgery,The Second Hospital,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Rui-Hai Liu,Food Science and Toxicology,Department of Food Science,Cornell University,Ithaca,NY 14853-7201,USA 《World Journal of Gastroenterology》 SCIE CAS CSCD 2002年第2期224-229,共6页
AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell... AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/waf1) of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 micromol.L(-1))of c 9, t 11-CLA for 24 and 48h, with a negative control (0.1% ethane). RESULTS: The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9, t11-CLA.SGC-7901 cells. Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5.92%, 20.15%, 75.61% and 82.44%, respectively and inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 micromol.L, 24h) showed significantly less (3)H-TdR incorporation than that in the negative controls (P【0.05 and P【0.01). Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%, 24h and 9.1-0.9% at 48h, respectively), Cyclin A (11.0-2.3%, 24h and 8.5-0.5%,48h), B(1) (4.8-1.8% at 24h and 5.5-0.6% at 48h)and D(1) (3.6-1.4% at 24h and 3.7%-0 at 48h) as compared with those in the negative controls(the expressions of PCNA, Cyclin A, B(1) and D(1) were 6.5% at 24h and 9.0% at 48h, 4.2% at 24h and 5.1% at 48h, 9.5% at 24h and 6.0% at 48h,respectively)(P【0.01), whereas the expressions of P16(ink4a) and P21(cip/waf1), cyclin-dependent kinases inhibitors(CDKI), were increased. CONCLUSION: The cell growth and proliferation of SGC-7901 cell is inhibited by c9, t11-CLA via blocking the cell cycle, with reduced expressions of cyclin A,B(1) and D(1) and enhanced expressions of CDKI(P16(ink4a) and p21(cip/waf1)). 展开更多
关键词 Linoleic Acids Conjugated ADENOCARCINOMA Animals Cell Cycle Cell Division Cyclin A Cyclin B Cyclin D1 Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinase Inhibitor p21 CYCLINS Enzyme Inhibitors Humans Immunohistochemistry Linoleic Acids Proliferating Cell Nuclear Antigen Research Support Non-U.S. Gov't Stomach Neoplasms Tumor Cells Cultured
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Preparation of Conjugated Linoleic Acid and Identification of Its Isomers 被引量:9
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作者 郭诤 张根旺 孙彦 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2003年第2期130-135,共6页
Conjugated linoleic acid (CLA) is a kind of fatty acid with physiological activities and potential application prospect. A synthesis method of conjugated linoleic acid and a purification technology were studied. CLA w... Conjugated linoleic acid (CLA) is a kind of fatty acid with physiological activities and potential application prospect. A synthesis method of conjugated linoleic acid and a purification technology were studied. CLA was prepared and purified by urea-complexation and conjugation using safflower oil as raw material. The purity of CLA and total recovery of the product was more than 95% and 48%, respectively. The main isomers produced in alkali-catalyzed conjugation were identified by gas chromatography (GC) linked to mass spectrometry (MS) and Fourier transform infrared spectroscopy (FTIR). The total amount of the two main isomers (9cis, 11trons-and 10trans, 12cis-CLA) determined by GC was more than 90% of the product. 展开更多
关键词 linoleic acid conjugated linoleic acid urea-complexation alkali-catalyzed conjugation IDENTIFICATION safflower oil
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Linoleic Acid Activates GPR40/FFA1 and Phospholipase C to Increase [Ca^(2+)]_i Release and Insulin Secretion in Islet Beta-Cells 被引量:2
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作者 Yi-jun Zhou Yu-ling Song +1 位作者 Hui Zhou Yan Li 《Chinese Medical Sciences Journal》 CAS CSCD 2012年第1期18-23,共6页
Objective To elucidate GPR40/FFA1 and its downstream signaling pathways in regulating insulin secretion. Methods GPR40/FFA1 expression was detected by immunofluorescence imaging. We employed linoleic acid (LA), a free... Objective To elucidate GPR40/FFA1 and its downstream signaling pathways in regulating insulin secretion. Methods GPR40/FFA1 expression was detected by immunofluorescence imaging. We employed linoleic acid (LA), a free fatty acid that has a high affinity to the rat GPR40, and examined its effect on cytosolic free calcium concentration ([Ca2+]i) in primary rat β-cells by Fluo-3 intensity under confocal microscopy recording. Downregulation of GPR40/FFA1 expression by antisense oligonucleotides was performed in pancreatic β-cells, and insulin secretion was assessed by enzyme-linked immunosorbent assay. Results LA acutely stimulated insulin secretion from primary cultured rat pancreatic islets. LA induced significant increase of [Ca2+]i in the presence of 5.6 mmol/L and 11.1 mmol/L glucose, which was reflected by increased Fluo-3 intensity under confocal microscopy recording. LA-stimulated increase in [Ca2+]i and insulin secretion were blocked by inhibition of GPR40/FFA1 expression in β-cells after GPR40/FFA1-specific antisense treatment. In addition, the inhibition of phospholipase C (PLC) activity by U73122, PLC inhibitor, also markedly inhibited the LA-induced [Ca2+]i increase. Conclusion LA activates GPR40/FFA1 and PLC to stimulate Ca2+ release, resulting in an increase in [Ca2+]i and insulin secretion in rat islet β-cells. 展开更多
关键词 GPR40 / FFA 1 phospholipase C antisense oligonucleotides intracellular calcium linoleic acid
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Qualitative and Quantitative Analysis of Linoleic Acid in Polygonati Rhizoma 被引量:1
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作者 ZHOU Zhun YUAN Han-Wen +8 位作者 PENG Cai-Yun JIAN Yu-Qing ZHOU Xu-Dong LI Bin SHENG Wen-Bing GONG Li-Min HE Shu-Jin WANG Wei LIU Chang-Xiao 《Digital Chinese Medicine》 2020年第3期180-187,共8页
Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently... Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精). 展开更多
关键词 Polygonati Rhizoma(Huang Jing 黄精) Linoleic acid Qualitative analysis Quantitative analysis Thin layer chromatography(TLC) High performance liquid chromatography(HPLC)
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Studies on mechanism of cis9,trans11-CLA and trans10,cis12-CLA inducing apoptosis of human breast cancer cell line MCF-7
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作者 Xianzi Wan Xianlin Yuan Xiangling Yang Yichen Li Ling Zhong 《The Chinese-German Journal of Clinical Oncology》 CAS 2010年第10期583-589,共7页
Objective: The aim of the study was to explore the activities of cis9, trans11-CLA (C9, t11-CLA) and transl0, cis12-CLA (t10, c12-CLA) inhibiting tumor, and investigate their relationships with PPARy and apoptoti... Objective: The aim of the study was to explore the activities of cis9, trans11-CLA (C9, t11-CLA) and transl0, cis12-CLA (t10, c12-CLA) inhibiting tumor, and investigate their relationships with PPARy and apoptotic proteins, and mechanism of anti-cancer. Methods: The inhibitory rate, cell growth curve and apoptotic morphological observation of MCF-7 cells were obtained by MTT assay, trypan blue staining and Hoechst33342 fluorescence staining. The apoptotic rate and cell cycle were detected with flow cytometry. Transcriptional level of genes was detected with RT-PCR semi-quantitative method, and Western blot was performed to detect proteins levels. Results: The two CLA isomers could reduce cell proliferation (P 〈 0.05), increase apoptotic rate (P 〈 0.05), and increase obviously the transcriptional and protein levels of PPARy (P 〈 0.01). The synchronism and correlation between the effects of CLA to PPARy and apoptotic proteins Bax, Bcl-2, Caspase 3 changes were found with the dose- and time-dependent manners. There was cooperative relation between the levels of PPARy and the rates of Bax/Bcl-2, Caspase 3 (small fragment) by experiments of PPARy inhibitor GW9662 and ligand Rosiglitazone. Conclusion: The apoptotic pathway of PPARy-Bcl-2-Caspase 3 signaling was found. The C9, t11-CLA and tl0, c12-CLA could inhibit MCF-7 cell proliferation and promote apoptosis via activating PPARy-Bcl-2-Caspase 3 pathway. CLA may be a kind of activator of PPARv. 展开更多
关键词 conjugated linoleic acid (CLA) isomer peroxisome proliferators activated receptor y (PPARγ) APOPTOSIS human breast cancer cell line MCF-7
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GC-MS Spectrums and Their Close Match Methyl Esters from Mushrooms of Papua New Guinea
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作者 John Kia Nema Stewart Wabo Wossa +1 位作者 Basil Marasinghe Russell Allan Barrow 《Journal of Chemistry and Chemical Engineering》 2014年第12期1093-1097,共5页
The GC-MS spectrums of a sample from Amanitaceae family and Mycenaceae family were matched with their close match GC-MS spectrums from the spectrum library. The samples from Amanitaceae family (KMsp027) and Mycenace... The GC-MS spectrums of a sample from Amanitaceae family and Mycenaceae family were matched with their close match GC-MS spectrums from the spectrum library. The samples from Amanitaceae family (KMsp027) and Mycenaceae family (KMsp039) were found to contain fatty acid methyl esters (methyl palmitate and methyl linoleate). It is proposed that the presence of methyl linoleate and methyl palmitate in the samples may show antibacterial activities if tested against different bacterial strains. 展开更多
关键词 Methyl esters GC-MS spectrum Papua New Guinea.
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Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system
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作者 衣丹 孙修勤 +3 位作者 李光友 刘发义 林学政 沈继红 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第3期574-577,共4页
We compared four esterifiable enzymes. The lipase Novozym 435 possessed the highest activity for the conjugated linoleic acid esterification during the synthesis of triglycerides. The triglycerides were synthesized by... We compared four esterifiable enzymes. The lipase Novozym 435 possessed the highest activity for the conjugated linoleic acid esterification during the synthesis of triglycerides. The triglycerides were synthesized by esterification of glycerol and conjugated linoleic acid (CLA) in a no-solvent system using lipase catalysis. We investigated the effects of temperature, enzyme concentration, water content, and time on esterification. Enzyme and water concentrations of up to 1% of the total reaction volume and a system temperature of 60℃ proved optimal for esterification. Similarly, when the esterification was carried out for 24 h, the reaction ratio improved to 94.11%. The esterification rate of the rotating screen basket remained high (87.28%) when the enzyme was re-used for the 5th time. We evaluated the substrate selectivity of lipase (NOVO 435) and determined that this lipase prefers the 10,12-octadacadienoic acid to the 9,11-octadecadienoic acid. 展开更多
关键词 conjugated linoleic acid GLYCEROL LIPASE ESTERIFICATION gyrating sieve
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Removal of nickel(Ⅱ) from aqueous solutions using iminodiacetic acid functionalized polyglycidyl methacrylate grafted-carbon fibers
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作者 付亚 吴家安 +1 位作者 周洪安 晋冠平 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2015年第6期919-923,共5页
Iminodiacetic acid functionalized polyglycidyl methacrylate grafted-carbon fibers(PGMA-IDA/CFs) were prepared for Ni(II) removal from aqueous solutions. The effects of solution p H value, temperature and adsorption ti... Iminodiacetic acid functionalized polyglycidyl methacrylate grafted-carbon fibers(PGMA-IDA/CFs) were prepared for Ni(II) removal from aqueous solutions. The effects of solution p H value, temperature and adsorption time were investigated. The maximum adsorption capacity of Ni(II) on PGMA-IDA/CFs is 0.923 mmol·L-1· g-1at pH 5.2 and 50 °C. Kinetic data indicate that the adsorption process matches the pseudo-second-order model and Elovich kinetic model. Thermodynamic data suggest that the adsorption process is endothermic spontaneous reaction. 展开更多
关键词 lminodiacetic acid Glyddyl methacrylate Carbon fiber Adsorption Nickel(II)
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Effect of Supplementation of Conjugated Linoleic Acid in Diets on Growth Performance and Total Lactic Bacteria iin Small Intestine of Broiler
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作者 T. Chanuwat S. Wongsuthavas B. Smerjai C. Yuangklang K. Vasupen 《Journal of Agricultural Science and Technology(A)》 2011年第8期1141-1143,共3页
The effect of supplementation of conjugated linoleic acid (CLA) in diets on growth performance and total lactic bacteria (LAB) in small intestine of broiler. One hundred sixty eight broilers (Arbor Acres; 1 wk ol... The effect of supplementation of conjugated linoleic acid (CLA) in diets on growth performance and total lactic bacteria (LAB) in small intestine of broiler. One hundred sixty eight broilers (Arbor Acres; 1 wk old) were randomly assigned to 8 groups. Each group (male 21 and female 21 broiler chickens) was further randomly divided into 3 replicates of 7 chickens were assigned to 4 dietary treatments (0%, 0.5%, 1.0%, and 1.5% CLA). Feed consumption and BW were recorded at every 7-day period. On day 56, and each week were collected at ileal content in small intestine for the total count of lactic acid bacteria (LAB). There were highly significant differences in body weight gain, average dairy feed intake, average dairy gain and feed conversion ratio among the treatments (P 〈 0.01). Moreover, our results revealed that the highest detection trend from the ileal occurred during the period in the supplementation of CLA. 展开更多
关键词 BROILER conjugated linoleic acid growth performance lactic acid bacteria.
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Effect of Palm Oil By-pass Fat on Milk Composition of Early Lactation Holstein Cows Fed Whole Plant Corn Silage during Dry Season
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作者 Y. Han R Paengkoum D. F. Wang 《Journal of Agricultural Science and Technology(A)》 2011年第8期1144-1149,共6页
The objective of this study was to investigate the effect of Palm Oil By-pass Fat (POBF) on milk composition of early lactation Holstein during dry season, 24 postpartum Holstein (1 d-14 d) were selected and align... The objective of this study was to investigate the effect of Palm Oil By-pass Fat (POBF) on milk composition of early lactation Holstein during dry season, 24 postpartum Holstein (1 d-14 d) were selected and align to two groups according to CRD experimental design; each group included 12 Holstein cows. The control received by-pass fat 0 g/h.d, the experimental group received by-pass fat 300 g/h.d. The results shown, DMI and yield of milk did not be affected with supplementation of by-pass fat, however, milk protein increased by 6.71%, milk fat increased by 8.16%, lactose increased by 0.22%, SNF increased by 1.98%, TS increased 4.08%; digestibility of diet protein decreased by 6.62%, digestibility of EE decreased by 7.96%, digestibility of NDF and ADL decreased by 1.36%, 8.97% respectively, digestibility of DM and OM decreased by 4.42, 3.07 percentage units; amount of C4-C17 milk fatty acids showed a decreased tendency, that of CIs-C22 showed an increased tendency, the milk CLA increased significantly (P 〈 0.01). To sum up, supplementation of by-pass fat for early lactation Holstein is an important measure to balance energy and sustain milk yield and quality. 展开更多
关键词 By-pass fat milk yield milk fat milk protein milk composition.
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Study on Microwave Preparation of Conjugated Linoleic Acid from Corn Germ Oil
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作者 WANG Zhen-qiang SHEN Sen FAN Xin 《Chinese Food Science》 2012年第1期7-9,共3页
[Objective] The optimal craft of preparing conjugated linoleic acid from corn germ oil by dint of microwave method was studied. [Meth- od] Microwave power, microwave mode of action, the amount of catalyst and solvent ... [Objective] The optimal craft of preparing conjugated linoleic acid from corn germ oil by dint of microwave method was studied. [Meth- od] Microwave power, microwave mode of action, the amount of catalyst and solvent were selected for single-factor-experiment. Then, based on the single-factor-experiment, the catalyst and solvent amount, and microwave function time were chosen for orthogonal experiment so as to deter- mine the optimal craft of preparing CLA from corn germ oil. UV absorption spectra and infrared absorption spectrum were used to analyse the struc- tural features of natural corn germ oil and CLA. [Resalt] The optimal preparation condition was as follow: the catalyst was 0.6 times heavier than oil, solvent was 3 times heavier than oil weight, microwave worked for ten minutes. Under the optimal condition, CLA was 56.3% and conversion rate was 99.47 %. The results indicated that non-conjugated double bonds of linoleic acid can be isomerized and changed into conjugated double bonds under the conditions of microwave irradiation,[ Conduslon] The study provided a new way for the preparation of CLA. 展开更多
关键词 MICROWAVE Corn germ oil PREPARATION Conjugated linoleic acid China
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Assessing the Oxidative Stability of Commercial Chia Oil
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作者 Maria A. Grompone Bruno Irigaray +1 位作者 Denisse Rodriguez Norma Sammain 《Journal of Food Science and Engineering》 2013年第7期349-356,共8页
The fatty acid composition as well as the antioxidant composition and content of two commercially available chia oils of different origins were studied. The purpose of this work was the study of the oxidative stabilit... The fatty acid composition as well as the antioxidant composition and content of two commercially available chia oils of different origins were studied. The purpose of this work was the study of the oxidative stability from different methods and the antioxidant content of the chia oils compared with other commercial oils. The oxidative stability of the oils was determined based on the oxidative stability index test (OSI test) conducted at 110 ~C and isothermal as well as non-isothermal differential scanning calorimeter (non-isothermal DSC) for the chia oil and the linseed oil. The OSI induction time of chia oil was compared with that of commercially available linseed, canola, sunflower and high-oleic sunflower oils, chia oil being the least stable oil among those studied. The inherent stability value and oxidazibility for linseed oil was lower than the chia oil A may be ascribed to a lower linolenic acid content of the former. The induction time (It) quotient at 110 ~C calculated for chia oil A and linseed oil were similar, suggesting a high degree of consistency between the results obtained by the two methods. The activation energy and specific reaction rate constant of chia and linseed oils were compared based on the results of isothermal and non-isothermal DSC. An apparent inconsistency in the experimental data results from the temperature-dependence of the activation energy of each fatty acid which can explain because the methods conditions were different. 展开更多
关键词 CHIA OIL TOCOPHEROL OXIDATION DSC.
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Influence of Replacement Time and Rate of Soybean Oil by Flax Oil in the Leghorn Hens' Diet on Polyunsaturated Fatty Acid Profile in Eggs
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作者 Nguyen Duy Hoan 《Journal of Agricultural Science and Technology(A)》 2015年第2期130-135,共6页
The study was aimed to evaluate the effect of replacement time and rate of soybean oil by flax oil (FO) on polyunsaturated fatty acid (PUFA) profile in egg of laying hens. Two hundred White Leghorn hens with 30 we... The study was aimed to evaluate the effect of replacement time and rate of soybean oil by flax oil (FO) on polyunsaturated fatty acid (PUFA) profile in egg of laying hens. Two hundred White Leghorn hens with 30 weeks of age were divided into five treatments with four replicates of l 0 birds each. Treatments were assigned randomly and consisted of 0.00%, 0.75%, 1.50%, 2.25% and 3.00% FO in commercial corn-soybean meal diets, in which the soybean oil was partially replaced. The experiment was conducted for 90 d. The main research findings were that feed intake, egg production rate, egg weight and feed conversion were not influenced by time and dietary treatment. Fatty acid content was significantly altered (P 〈 0.05) by FO, showing a progressive increase in egg n-3 fatty acid (especially docosahexaenoic acids (DHA) and eicosapentaenoic acids (EPA)) when FO was added. Levels of EPA and DHA were higher (P 〈 0.05) in the egg lipids of FO fed hens than those in the control group. However, no significant differences were observed either in egg weight or egg production among groups. The highest incorporation (P 〈 0.05) of total n-3 fatty acid content in eggs was obtained with 3% FO/kg. This increase was proportional to FO inclusion levels in the diets. 展开更多
关键词 Egg enriched laying hens n-3 and n-6 fatty acids FO.
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Density estimated physicochemical properties of alanine-based ionic liquid [C_7mim][Ala] and its application in selective transesterification of soybean oil 被引量:2
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作者 FANG DaWei LI Meng +3 位作者 GE RiLe ZANG ShuLiang YANG JiaZhen GAO YanAn 《Science China Chemistry》 SCIE EI CAS 2012年第8期1677-1682,共6页
Amino acid ionic liquid (AAIL) based on alanine, 1-n-heptyl-3-methylimidazolium alanine ([C7mim][Ala]), has been prepared and characterized. Since the IL can form strong hydrogen bonds with water, trace amount of ... Amino acid ionic liquid (AAIL) based on alanine, 1-n-heptyl-3-methylimidazolium alanine ([C7mim][Ala]), has been prepared and characterized. Since the IL can form strong hydrogen bonds with water, trace amount of water is a problematic impurity in the IL. Using the standard addition method (SAM), the densities were measured in the temperature range from 293.15 to 343.15±0.05 K. On the basis of the experimental data of density, the surface tension y, molar volume, and the molecular vol- ume Vm, molar enthalpy of vaporization AIgHm0, and the thermal expansion coefficients or, for [C7mim][Ala] were estimated using semi-empirical methods. Also the interstice model has been verified with good accordance. Then the [C7mim][Ala] was taken as catalyst in the transesterification reaction with fatty acid, where soybean oil was used as raw material and methanol as reactant. The amount of catalyst, alcohol consumption, reaction time, temperature and other factors of the reaction were inves- tigated. Using GC determination, the conversions were calculated by the internal standard method. The main ingredients of the products were identified by GC-MS as palm methyl ester and linoleic acid methyl ester only, which showed high selectivity in palmitic acid and linoleic acid. 展开更多
关键词 ALANINE ionic liquid density TRANSESTERIFICATION
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