Besides the major 250 kD PEPCase isoform, a 300 kD minor PEPCaseform was also found in Mesembryanthemum cordifolium leaves (Fig. 1 ). Byusing SDS PAGE it was found that bothisoforms were homodiners. ’The 250 kDform c...Besides the major 250 kD PEPCase isoform, a 300 kD minor PEPCaseform was also found in Mesembryanthemum cordifolium leaves (Fig. 1 ). Byusing SDS PAGE it was found that bothisoforms were homodiners. ’The 250 kDform consisted of two 100 kD subunits ,and the 300 kD form is a dimer of two110 kD subunits (Figs. 2,3). ’The discrepency between molectilar weights ofthe native enzymes and their subunitswas a result of the different molecularweight determinating methods. There fore, there is no heterodimer of PEPCase in this plant. A 1 080 kD PEPCase form was found in both saltstressed and well-watered leaf samples.This PEPCase is an octamer, and therewas no diurnal fluctuation in its abundance.展开更多
文摘Besides the major 250 kD PEPCase isoform, a 300 kD minor PEPCaseform was also found in Mesembryanthemum cordifolium leaves (Fig. 1 ). Byusing SDS PAGE it was found that bothisoforms were homodiners. ’The 250 kDform consisted of two 100 kD subunits ,and the 300 kD form is a dimer of two110 kD subunits (Figs. 2,3). ’The discrepency between molectilar weights ofthe native enzymes and their subunitswas a result of the different molecularweight determinating methods. There fore, there is no heterodimer of PEPCase in this plant. A 1 080 kD PEPCase form was found in both saltstressed and well-watered leaf samples.This PEPCase is an octamer, and therewas no diurnal fluctuation in its abundance.
