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中国人戈谢病基因突变的分析 被引量:38
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作者 张为民 邓亮生 +5 位作者 孟岩 姚凤霞 邱正庆 段彦龙 黄尚志 施惠平 《中华医学杂志》 CAS CSCD 北大核心 2009年第48期3397-3400,共4页
目的回顾及分析中国人戈谢病(Gaucher disease,GD)的基因突变类型,观察其与临床表型的相关性。方法用基因分析的方法包括用特异限制性内切酶酶切PCR产物,检测中国人戈谢病患者已知突变位点;对含有未知突变基因的个体采用长链PCR... 目的回顾及分析中国人戈谢病(Gaucher disease,GD)的基因突变类型,观察其与临床表型的相关性。方法用基因分析的方法包括用特异限制性内切酶酶切PCR产物,检测中国人戈谢病患者已知突变位点;对含有未知突变基因的个体采用长链PCR方法,分段扩增葡萄糖脑苷脂酶的功能基因,再用巢式PCR分别扩增功能基因的外显子序列,进行DNA序列测定。结果总结本实验室检测到的40种突变类型,常见的突变等位基因为L444P,占突变基因的33.0%,其他较常见的突变为F213I、N188S、V375L和M416V。结论中国人GD患者中起码存在40种突变类型,基因突变类型谱与白种人有明显的区别。目前已明确70%的基因突变类型,通过基因分析进行GD的临床诊断和产前诊断已成为可能。 展开更多
关键词 戈谢病 酸性β-葡萄糖苷酶 突变类型 中国人
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Use of a Bioaugmented Organic Soil Amendment in Combination with Gypsum for Withania somnifera Growth on Sodic Soil 被引量:7
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作者 Manjul GUPTA Pankaj Kumar SRIVASTAVA +2 位作者 SHIKHA Abhishek NIRANJAN Shri Krishna TEWARI 《Pedosphere》 SCIE CAS CSCD 2016年第3期299-309,共11页
Limited availability of organic matter is a problem to sustain crop growth on sodic soil. Organic soil amendments are a costeffective source of nutrients to enhance crop growth. A field study was conducted to evaluate... Limited availability of organic matter is a problem to sustain crop growth on sodic soil. Organic soil amendments are a costeffective source of nutrients to enhance crop growth. A field study was conducted to evaluate the effect of an organic soil amendment bioaugmented with plant growth-promoting fungi(SF_(OA) ) in combination with gypsum on soil properties and growth and yield attributes of Withania somnifera, one of the most valuable crops of the traditional medicinal system in the world, on a sodic soil at the Aurawan Research Farm of CSIR-National Botanical Research Institute, Lucknow, India. The SF_(OA) used was prepared by pre-enriching farm waste vermicompost with plant growth-promoting fungi before mixing with pressmud and Azadirachta indica seed cake. The application of SF_(OA) at 10 Mg ha^(-1)after gypsum(25.0 Mg ha^(-1)) treatment significantly(P < 0.05) increased root length(by 96%) and biomass(by 125%) of Withania plants compared to the control without SF_(OA) and gypsum. Similarly, the highest withanolide contents were observed in leaves and roots of Withania plants under 10 Mg ha^(-1)SF_(OA) and gypsum. Combined application of SF_(OA) and gypsum also improved physical, chemical and enzymatic properties of the soil, with the soil bulk density decreasing by 25%, water-holding capacity increasing by 121%, total organic C increasing by 90%, p H decreasing by 17% and alkaline phosphatase, β-glucosidase, dehydrogenase and cellulase activities increasing by 54%, 128%, 81% and 96%, respectively, compared to those of the control. These showed that application of the SF_(OA) tested in this study might reclaim sodic soil and further support Withania cultivation and results were better when the SF_(OA) was applied after gypsum treatment. 展开更多
关键词 medicinal crop plant growth-promoting fungi soil property soil reclamation vermicompost
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