以污水处理厂污泥的酸性发酵产物为硫酸盐还原菌(SRB)的碳源,在厌氧膨胀颗粒污泥床(EGSB)反应器中,研究了生物处理模拟酸性矿山废水(AMD)的工艺特性及影响因素。试验结果表明,污水厂污泥的酸性发酵产物可作为SRB的合适碳源。常温(20℃)...以污水处理厂污泥的酸性发酵产物为硫酸盐还原菌(SRB)的碳源,在厌氧膨胀颗粒污泥床(EGSB)反应器中,研究了生物处理模拟酸性矿山废水(AMD)的工艺特性及影响因素。试验结果表明,污水厂污泥的酸性发酵产物可作为SRB的合适碳源。常温(20℃)条件下,当AMD中SO24-浓度为3000 mg.L-1,pH值3.0,EGSB反应器中液体升流速度为5.0 m.h-1,水力停留时间HRT=13.8 h,碳源COD/SO24-比值取1.0左右,进水SO24-负荷为5.22 kg SO24-.m-3.d-1时,SRB的还原能力可达到3.32 kg SO24-.m-3.d-1,SRB的比还原能力为0.356 kg SO24-.(kg VSS)-1.d-1。AMD处理出水pH值可达6.0,SO24-还原率达到63.6%,COD去除率为45.1%,重金属Fe2+、Mn2+、Ni2+、Zn2+、Cu2+去除率均在89%以上。出水pH值和重金属离子浓度均满足排放标准。展开更多
A yeast strain R6 was obtained by the method of thiamine（VB1） auxotrophic negative selection from the edible oil-polluted soil in Zibo, China. Physiological and biochemical experiments revealed that strain R6 shared...A yeast strain R6 was obtained by the method of thiamine（VB1） auxotrophic negative selection from the edible oil-polluted soil in Zibo, China. Physiological and biochemical experiments revealed that strain R6 shared common feature with Rhodotorula mucilaginosa according to the API 20 C AUX yeast identification system which has been tested previously. Furthermore, the 18 S r DNA gene of strain R6 was amplified and sequenced. Phylogenetic analysis based on the 18 S r DNA sequence and the relatives indicated that R6 shared 99% homologies with the members of R. mucilaginosa, suggesting that strain R6 belonged to R. mucilaginosa.Investigation showed that strain R6 possessed the capacity of accumulating exocellular alpha-ketoglutaric acid（alpha-KG）. Finally, the fermentation conditions of R6 to accumulate alpha-KG was optimized by controlling each single fermenting variable and detected through high performance liquid chromatography（HPLC）. Results showed that both VB1 and Ca CO3 in fermentation medium were the key factors influencing the cumulant of alpha-KG. The discovery of natural auxotrophic strain R6 not only broadened the microbial resource which can achieve lots of alpha-KG production through fermentation, but also laid a foundation for further fermentation regulation to achieve excessive alpha-KG accumulation.展开更多
The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Opt...The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.展开更多
Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biolog...Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biological functions and expected that fermented extract by lactobacilli will be superior to non-fermented extract in antioxidant activity. C. versicolor was extracted with 70% ethanol and then it was fermented by Lactobacillus acidophilus KCTC3164 and Lactobacillus plantarum KCTC3099, respectively. 1,l-dipheny｜-2-picrylhydrazyl （DPPH） scavenging activity, superoxide dismutase （SOD）-like activity and tetrazolium salt 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assays were performed. DPPH scavenging activity of fermented extract by L. acidophilus was higher than fermented extract by L. plantarum and antioxidant effect of fermented extract was 20%-30% higher than non-fermented extract. SOD-like activity was represented 18% in fermented extract by L. acidophilus, and was 27% in fermented extract by L. plantarum higher than non-fermented extract. As a result, this study suggests that it may need an efficient protocol for dominant bio-physiological activities such as extract volume or appropriate working volume of cultured cell or mixture condition. The fermented C. versicolor extract should provide with useful antioxidant agent in the human skin.展开更多
文摘以污水处理厂污泥的酸性发酵产物为硫酸盐还原菌(SRB)的碳源,在厌氧膨胀颗粒污泥床(EGSB)反应器中,研究了生物处理模拟酸性矿山废水(AMD)的工艺特性及影响因素。试验结果表明,污水厂污泥的酸性发酵产物可作为SRB的合适碳源。常温(20℃)条件下,当AMD中SO24-浓度为3000 mg.L-1,pH值3.0,EGSB反应器中液体升流速度为5.0 m.h-1,水力停留时间HRT=13.8 h,碳源COD/SO24-比值取1.0左右,进水SO24-负荷为5.22 kg SO24-.m-3.d-1时,SRB的还原能力可达到3.32 kg SO24-.m-3.d-1,SRB的比还原能力为0.356 kg SO24-.(kg VSS)-1.d-1。AMD处理出水pH值可达6.0,SO24-还原率达到63.6%,COD去除率为45.1%,重金属Fe2+、Mn2+、Ni2+、Zn2+、Cu2+去除率均在89%以上。出水pH值和重金属离子浓度均满足排放标准。
基金Supported by the Natural Science Foundation of Shandong Province,China(ZR2010CQ017)Program of Young Teachers Development of Shandong University of Technology~~
文摘A yeast strain R6 was obtained by the method of thiamine（VB1） auxotrophic negative selection from the edible oil-polluted soil in Zibo, China. Physiological and biochemical experiments revealed that strain R6 shared common feature with Rhodotorula mucilaginosa according to the API 20 C AUX yeast identification system which has been tested previously. Furthermore, the 18 S r DNA gene of strain R6 was amplified and sequenced. Phylogenetic analysis based on the 18 S r DNA sequence and the relatives indicated that R6 shared 99% homologies with the members of R. mucilaginosa, suggesting that strain R6 belonged to R. mucilaginosa.Investigation showed that strain R6 possessed the capacity of accumulating exocellular alpha-ketoglutaric acid（alpha-KG）. Finally, the fermentation conditions of R6 to accumulate alpha-KG was optimized by controlling each single fermenting variable and detected through high performance liquid chromatography（HPLC）. Results showed that both VB1 and Ca CO3 in fermentation medium were the key factors influencing the cumulant of alpha-KG. The discovery of natural auxotrophic strain R6 not only broadened the microbial resource which can achieve lots of alpha-KG production through fermentation, but also laid a foundation for further fermentation regulation to achieve excessive alpha-KG accumulation.
文摘The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.
文摘Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biological functions and expected that fermented extract by lactobacilli will be superior to non-fermented extract in antioxidant activity. C. versicolor was extracted with 70% ethanol and then it was fermented by Lactobacillus acidophilus KCTC3164 and Lactobacillus plantarum KCTC3099, respectively. 1,l-dipheny｜-2-picrylhydrazyl （DPPH） scavenging activity, superoxide dismutase （SOD）-like activity and tetrazolium salt 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assays were performed. DPPH scavenging activity of fermented extract by L. acidophilus was higher than fermented extract by L. plantarum and antioxidant effect of fermented extract was 20%-30% higher than non-fermented extract. SOD-like activity was represented 18% in fermented extract by L. acidophilus, and was 27% in fermented extract by L. plantarum higher than non-fermented extract. As a result, this study suggests that it may need an efficient protocol for dominant bio-physiological activities such as extract volume or appropriate working volume of cultured cell or mixture condition. The fermented C. versicolor extract should provide with useful antioxidant agent in the human skin.
