To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach...To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach. Eight acid mine water samples were collected from five areas in Tongling. Phylogenetic analyses revealed that bacteria mainly fell into ten divisions, which were Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi. Archaea fell into three phylogenetic divisions, Thermoplasma, Ferroplasma and Thermogymnomonas. The unweighted pair group method with arithmetic mean（UPGMA） cluster analysis based on the microbial communities’ compositions revealed that five samples shared similarity with the dominance of Meiothermus and Thermomonas. Two samples had the preponderant existence of Acidithiobacillus and Leptospirillum. The remaining sample owned higher microbial communities’ diversity with the Shannon-Weaver H up to 2.91. Canonical correlation analysis（CCA） suggested that microbial community structures had great association with p H and the concentration of Hg2＋, Pb2＋, Fe3＋, Cl-, SO2- 4in water.展开更多
基金Project(41171418)supported by the National Natural Science Foundation of China
文摘To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach. Eight acid mine water samples were collected from five areas in Tongling. Phylogenetic analyses revealed that bacteria mainly fell into ten divisions, which were Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi. Archaea fell into three phylogenetic divisions, Thermoplasma, Ferroplasma and Thermogymnomonas. The unweighted pair group method with arithmetic mean（UPGMA） cluster analysis based on the microbial communities’ compositions revealed that five samples shared similarity with the dominance of Meiothermus and Thermomonas. Two samples had the preponderant existence of Acidithiobacillus and Leptospirillum. The remaining sample owned higher microbial communities’ diversity with the Shannon-Weaver H up to 2.91. Canonical correlation analysis（CCA） suggested that microbial community structures had great association with p H and the concentration of Hg2＋, Pb2＋, Fe3＋, Cl-, SO2- 4in water.
