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酸性矿山水处理技术的新进展 被引量:8
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作者 杨文甫 祝玉学 《国外金属矿山》 2001年第4期60-64,共5页
介绍了第五届国际酸性岩石水会议的最新动态 ,重点介绍 3个实例 :( 1)萨姆特维尔露天金矿采用传统方法处理废弃的扰动区 ;( 2 )利利 /奥劳矿采用生物硫酸盐还原方法就地处理酸性水并去除重金属 ;( 3)
关键词 酸性矿山水 处理 土地恢复 生物硫酸盐 硅酸盐覆盖技术
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细菌在矿山酸性水形成和治理中的作用 被引量:4
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作者 李建设 《矿冶》 CAS 1994年第3期32-35,共4页
矿山酸性废水是由于硫化矿物氧化而引起的污染问题,细菌加快了氧化反应的速度,在酸性水形成中起主要作用。由于氧化铁硫杆菌在Fe ̄(2+)氧化成Fe ̄(3+)时起催化作用,所以在酸性水处理中可利用细菌氧化代替空气氧化,再用... 矿山酸性废水是由于硫化矿物氧化而引起的污染问题,细菌加快了氧化反应的速度,在酸性水形成中起主要作用。由于氧化铁硫杆菌在Fe ̄(2+)氧化成Fe ̄(3+)时起催化作用,所以在酸性水处理中可利用细菌氧化代替空气氧化,再用CaCO_3使金属离子沉淀,从而降低了废水处理成本。 展开更多
关键词 矿山酸性 细菌 氧化 处理
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酸性水作用下露天金属矿山“类土质”边坡体试验
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作者 鞠海燕 张鹏 +1 位作者 赵江倩 刘少林 《南昌工程学院学报》 CAS 2015年第3期64-66,74,共4页
矿山酸性水作用是矿山边坡体破坏的一种特殊方式,通过对"类土质"边坡表层岩土体及深层岩土体在酸性水作用下进行剪切、抗压强度及纵波波速试验,结合某露天矿山边坡的工程地质条件及类土质边坡的地质特征,分析了露天矿山"... 矿山酸性水作用是矿山边坡体破坏的一种特殊方式,通过对"类土质"边坡表层岩土体及深层岩土体在酸性水作用下进行剪切、抗压强度及纵波波速试验,结合某露天矿山边坡的工程地质条件及类土质边坡的地质特征,分析了露天矿山"类土质"边坡在酸性水作用下的力学性质及失稳机理,为矿山边坡维护加固提供理论依据。 展开更多
关键词 露天矿山 类土质边坡 矿山酸性 失稳机理 试验
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应用高效浓密机处理高浓度矿山酸性水的研究 被引量:3
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作者 马效贤 《中国矿山工程》 2004年第5期17-19,共3页
原德兴铜矿工业水处理站的处理工艺由于石灰中和沉淀速度过慢等原因,处理水量只能达到设计规模的三分之一,处理效果不稳定,难于正常连续运转。通过试验,将原浓缩池(幅流式沉淀池)应用高效浓密机技术进行改造,改造后经半年的生产使用证明... 原德兴铜矿工业水处理站的处理工艺由于石灰中和沉淀速度过慢等原因,处理水量只能达到设计规模的三分之一,处理效果不稳定,难于正常连续运转。通过试验,将原浓缩池(幅流式沉淀池)应用高效浓密机技术进行改造,改造后经半年的生产使用证明,高效浓密机与改造前的浓密池相比,沉淀效率提高了一倍以上,而且处理效果稳定,达到了设计要求。 展开更多
关键词 高效浓密机 矿山酸性 沉降
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Acid mine drainage activation mechanism on lime-depressed pyrite flotation from copper sulfide ore
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作者 Jia-qiao YUAN Zhan DING +3 位作者 Yun-xiao BI Jie LI Shu-ming WEN Shao-jun BAI 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2024年第9期2987-3001,共15页
The lime-depressed pyrite from Cu differential flotation tailings with acid mine drainage(AMD)as a natural activator was recovered.The effect of AMD on lime-depressed pyrite flotation was investigated by a series of l... The lime-depressed pyrite from Cu differential flotation tailings with acid mine drainage(AMD)as a natural activator was recovered.The effect of AMD on lime-depressed pyrite flotation was investigated by a series of laboratory flotation tests and surface analytical techniques.Flotation test results indicated that AMD could effectively activate the pyrite flotation with a sodium butyl xanthate(SBX)collector,and a high-quality sulfur concentrate was obtained.Pulp ion concentration analysis results indicated that AMD facilitated desorption of Ca~(2+)and adsorption of Cu~(2+)on the depressed-pyrite surface.Adsorption measurements and contact angle analysis results confirmed that adding AMD improved the adsorption amount of SBX collector on the pyrite surface and increased the contact angle by 31°.Results of Raman spectroscopy and X-ray photoelectron spectroscopy analysis indicated that AMD treatment promoted the formation of hydrophobic species(S~0 hydrophobic entity and copper sulfides)and the removal of hydrophilic calcium and iron species on the pyrite surface,which reinforced the adsorption of collector.The findings of the present research provide important theoretical basis and technical support for a cleaner production of copper sulfide ores. 展开更多
关键词 copper sulfur ore acid mine drainage lime-depressed pyrite FLOTATION natural activator activation mechanism
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AMD蚀化下砂岩的损伤本构模型 被引量:16
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作者 姜立春 温勇 《中南大学学报(自然科学版)》 EI CAS CSCD 北大核心 2011年第11期3502-3506,共5页
通过酸性矿山排泄水(AMD)蚀化砂岩的力学性质研究,利用Weibull函数分析砂岩微元体强度分布特征,导出单轴压缩下砂岩的损伤本构关系;基于AMD蚀化下砂岩主要胶结物含量的变化,引入砂岩化学损伤变量,得到蚀化后砂岩的等效弹性模量;根据推广... 通过酸性矿山排泄水(AMD)蚀化砂岩的力学性质研究,利用Weibull函数分析砂岩微元体强度分布特征,导出单轴压缩下砂岩的损伤本构关系;基于AMD蚀化下砂岩主要胶结物含量的变化,引入砂岩化学损伤变量,得到蚀化后砂岩的等效弹性模量;根据推广的Lemaitre应变等效原理,建立AMD蚀化下砂岩的损伤本构模型。利用现场采集的pH为1.07和3.26的AMD溶液蚀化下砂岩单轴抗压试验,以及文献[7]中人工配制pH为3.0和5.0酸液溶蚀砂岩试验结果,分别检验所建本构模型的合理性。结果表明:模型预测曲线与试验结果曲线基本吻合,较客观地反映AMD蚀化下砂岩的损伤演化特性。 展开更多
关键词 岩石力学 酸性矿山排泄 化学损伤 本构模型 WEIBULL分布
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硫酸盐还原菌示范工程概述 被引量:20
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作者 M.坎迪 祝玉学 桂誉漓 《国外金属矿山》 2000年第4期62-68,共7页
介绍使用硫酸盐还原菌 (SRB)处理和控制酸性矿山排水 (AMD)新技术的半工业性试验和现场试验结果。当有碳和硫酸盐源供给时 ,硫酸盐还原菌 (一种普通的厌氧菌群 )生产出硫化氢和重碳酸盐。硫化氢与AMD中金属离子起反应 ,生成金属硫化物... 介绍使用硫酸盐还原菌 (SRB)处理和控制酸性矿山排水 (AMD)新技术的半工业性试验和现场试验结果。当有碳和硫酸盐源供给时 ,硫酸盐还原菌 (一种普通的厌氧菌群 )生产出硫化氢和重碳酸盐。硫化氢与AMD中金属离子起反应 ,生成金属硫化物而沉淀。生成的重碳酸盐用来促进酸性矿山排水的中和。半工业性试验金属去除系数达到 :Zn 99% ,Al 99% ,Mn 96 % ,Cd 98%和Cu 96 %。但Fe和As去除不如上述金属有效 ,这主要是由于污染有机给养基的铁和砷含量高。有证据说明 ,吸附作用和硫酸盐还原都在反应器中发生。SRB现场示范工程包括使用蒙大拿州埃利斯顿附近的利利 奥芬博伊矿淹没的地下矿山工作区作为“原地生物反应器”。该矿在 4年的监测期间 ,已观察到Al、Cd、Cu和Zn都有高的去除系数 ( 70 %~接近 1 0 0 % )。然而 ,由于与半工业性试验类似的原因 ,As和Fe测得低的去除系数。通过矿山水中硫酸盐减少的测定和可溶硫化物的检测说明 ,硫酸盐还原是明显的。 展开更多
关键词 硫酸盐还原菌 酸性矿山水 处理 SRB 金属离子 去除机理
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简讯
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作者 张晓云 《现代矿业》 CAS 1999年第4期23-25,共3页
从酸性矿山水中生产新鲜水澳大利亚矿山补救服务有限公司(MRS)将利用其高岭土非晶衍生物(KAD)技术从昆士兰州的莫尔干山(Mt Morgan)铜金矿的矿山酸性排放水中生产新鲜水。矿山补救服务有限公司是卡迪亚(Cardia)采矿公司(61%)与昆士兰大... 从酸性矿山水中生产新鲜水澳大利亚矿山补救服务有限公司(MRS)将利用其高岭土非晶衍生物(KAD)技术从昆士兰州的莫尔干山(Mt Morgan)铜金矿的矿山酸性排放水中生产新鲜水。矿山补救服务有限公司是卡迪亚(Cardia)采矿公司(61%)与昆士兰大学的商业化公司U-niQuest公司(31%)的合资公司。这项KAD技术是建立在由UniQest公司先进陶瓷材料开发部的伊恩麦金农(Ian Maackinnon) 展开更多
关键词 补救服务 昆士兰州 酸性矿山水 新鲜 半工业试验 澳大利亚矿 高岭土 先进陶瓷 排放 昆士兰大学
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减少AMD污泥排放的高浓度污泥处理方法
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作者 R.L.齐克 M.H.利昂 +2 位作者 D.C.芬恩 祝玉学 张绪珍 《国外金属矿山》 2000年第6期60-64,共5页
当处理金属废水时 ,浓污泥处理法是使废料最小化的方法 ,这种技术能大大减少废料产生 ,还能改善处理系统的性能 ,降低作业成本 。
关键词 酸性矿山水 pH值 高浓度污泥处理法 金属废
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德兴铜矿HDS底泥回用的试验研究 被引量:1
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作者 严思明 兰秋平 《铜业工程》 CAS 2015年第4期32-34,57,共4页
与石灰乳相比,采用HDS底泥调节化学硫化技术除铁工艺p H,可以提高铜回收率和三价铁离子去除率;铜回收工艺,硫氢化钠用量少,且铜总回收率也相对较高;HDS工艺,虽然石灰乳用量偏大,但是从整个工艺来看,每升酸性水少用石灰乳5-6g,并且相同... 与石灰乳相比,采用HDS底泥调节化学硫化技术除铁工艺p H,可以提高铜回收率和三价铁离子去除率;铜回收工艺,硫氢化钠用量少,且铜总回收率也相对较高;HDS工艺,虽然石灰乳用量偏大,但是从整个工艺来看,每升酸性水少用石灰乳5-6g,并且相同的铜上清液,添加HDS底泥比不添加要少用石灰乳1g多(约减少5%的石灰乳用量)。废水处理很少采用分段法,即用后段的底泥作为碱源调节前段的p H,降低石灰用量,来达到降低处理成本的目的。这样既减少石灰用量,又使底泥得到回用,带来了良好的经济效益。 展开更多
关键词 HDS底泥 石灰乳 矿山酸性 分段法 综合利用
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Heavy Metals in a Sulfldic Minespoil: Fractions and Column Leaching 被引量:16
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作者 C.LIN J.LIN 《Pedosphere》 SCIE CAS CSCD 2003年第1期75-80,共6页
Fractions of various heavy metals in a sulfidic minespoil were investigated. Column leaching experimentwas also conducted to simulate 'acid mine drainage' (AMD) from the minespoil. The results show thatleachin... Fractions of various heavy metals in a sulfidic minespoil were investigated. Column leaching experimentwas also conducted to simulate 'acid mine drainage' (AMD) from the minespoil. The results show thatleaching of heavy metals from the minespoil was extremely significant during the initial water flushing.The amounts of heavy metals leached out dramatically reduced after leaching twice. It is worthwhile tonote that in this study, Zn, Mn, Fe, As and Ni in the first leachate exceeded the total amount of eachcorresponding water-extractable (1:5, soil:water) metal contained in the minespoil sample. This appears tosuggest that 1:5 water extraction did not allow accurate estimation of water-leachable concentrations of theabove heavy metals. This work has implications for the management of sulfidic minespoils. Acid drainageof great environmental concerns is likely to occur only during heavy rainfall events after substantial solubleand readily exchangeable acid and metals are accumulated in the minespoils. The slow-reacting fractionsother than water-soluble and readily exchangeable fractions may pose little environmental hazards. This isparticularly true for Pb, As and Ni. 展开更多
关键词 acid mine drainage heavy metal LEACHING metal sulfides minespoil
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Acid mine drainage and heavy metal contamination in groundwater of metal sulfide mine at arid territory (BS mine,Western Australia) 被引量:12
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作者 雷良奇 宋慈安 +2 位作者 谢襄漓 李艳红 王飞 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第8期1488-1493,共6页
The issues of acid mine drainage (AMD) and heavy metals contamination in the metal sulfide mine in the add district were explored, through studying the acidification and the heavy metals distribution and evolution o... The issues of acid mine drainage (AMD) and heavy metals contamination in the metal sulfide mine in the add district were explored, through studying the acidification and the heavy metals distribution and evolution of groundwater in the black swan (BS) nickel sulfide mine (Western Australia). The groundwater samples were collected from the drilling holes situated in the vicinity of tailings storage facility (TSF) and in the background of the mine (away from TSF), respectively, and the pH and electric conductivity (Ec) were measured in site and the metal contents were analysed by ICP-MS and ICP-AES, quarterly in one hydrological year. The results disclose that the TSF groundwater is remarkably acidified (.pHmean=5, pHmin=3), and the average contents of heavy metals (Co, Cu, Zn, Cd) and Al, Mn are of 1-2 orders of magnitude higher in TSF groundwater than in background groundwater. It may be due to the percolation of tailings waste water from miU process, which leads the tailings to oxidize and the deep groundwater to acidify and contaminate with heavy metals. Besides, the heavy metals concentration in groundwater may be controlled by pH mainly. 展开更多
关键词 nickel ore tailings acid mine drainage heavy metals groundwater contamination
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Sulfide mineral bioleaching:Understanding of microbe-chemistry assisted hydrometallurgy technology and acid mine drainage environment protection 被引量:5
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作者 LIAO Rui YU Shi-chao +10 位作者 WU Bai-qiang ZHAO Chun-xiao LIN Hao HONG Mao-xin WU Hai-yan YANG Cong-ren ZHANG Yan-sheng XIE Jian-ping QIN Wen-qing WANG Jun QIU Guan-zhou 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第5期1367-1372,共6页
Bioleaching is regarded as an essential technology to treat low grade minerals,with the distinctive superiorities of lower-cost and environment-friendly compared with traditional pyrometallurgy method.However,the biol... Bioleaching is regarded as an essential technology to treat low grade minerals,with the distinctive superiorities of lower-cost and environment-friendly compared with traditional pyrometallurgy method.However,the bioleaching efficiency is unsatisfactory owing to the passivation film formed on the minerals surface.It is of particular interest to know the dissolution and passivation mechanism of sulfide minerals in the presence of microorganism.Although bioleaching can be useful in extracting metals,it is a double-edged sword.Metallurgical activities have caused serious environmental problems such as acid mine drainage(AMD).The understanding of some common sulfide minerals bioleaching processes and protection of AMD environment is reviewed in this article. 展开更多
关键词 BIOLEACHING sulfide minerals MICROORGANISM acid mine drainage
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Acidophilic bacterial community reflecting pollution level of sulphide mine impacted by acid mine drainage 被引量:5
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作者 万民熙 杨宇 +4 位作者 邱冠周 徐爱玲 钱林 黄芝英 夏金兰 《Journal of Central South University》 SCIE EI CAS 2009年第2期223-229,共7页
To reveal the impact of mining on bacterial ecology around mining area,bacterial community and geochemical characteristics about Dabaoshan Mine(Guangdong Province,China)were studied.By amplified ribosomal DNA restrict... To reveal the impact of mining on bacterial ecology around mining area,bacterial community and geochemical characteristics about Dabaoshan Mine(Guangdong Province,China)were studied.By amplified ribosomal DNA restriction analysis and phylogenetic analysis,it is found that mining pollution greatly impacts the bacterial ecology and makes the habitat type of polluted environments close to acid mine drainage(AMD)ecology.The polluted environment is acidified so greatly that neutrophil and alkaliphilic microbes are massively dead and decomposed.It provided organic matters that can make Acidiphilium sp.rapidly grow and become the most bacterial species in this niche.Furthermore,Acidithiobacillus ferrooxidans and Leptospirillum sp.are also present in this niche.The amount of Leptospirillum sp.is far more than that of Acidithiobacillus ferrooxidans,which indicates that the concentration of toxic ions is very high.The conclusions of biogeochemical analysis and microbiological monitor are identical. Moreover,because the growth of Acidithiobacillus ferrooxidans and Leptospirillum sp.depends on ferrous iron or inorganic redox sulfur compounds which can be supplied by continual AMD,their presence indicates that AMD still flows into the site.And the area is closer to the outfalls of AMD,their biomasses would be more.So the distinction of their biomasses among different areas can help us to find the effluent route of AMD. 展开更多
关键词 bacterial community acid mine drainage (AMD) Acidithiobacillusferrooxidans Leptospirillum sp.
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Diversity of microbial community at acid mine drainages from Dachang metals-rich mine, China 被引量:3
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作者 周智君 尹华群 +3 位作者 刘毅 谢明 邱冠周 刘学端 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第6期1097-1103,共7页
Two acid mine drainage(AMD)samples TS and WK,which were from the Dachang metals-rich mine in Guangxi province,China,were studied using PCR-based cloning approach.A total of 44 operational taxonomic units(OTUs)were obt... Two acid mine drainage(AMD)samples TS and WK,which were from the Dachang metals-rich mine in Guangxi province,China,were studied using PCR-based cloning approach.A total of 44 operational taxonomic units(OTUs)were obtained from the two AMD samples.However,only three OTUs(GXDC-9,GXDC-19 and GXDC-50)detected in sample TS can also be observed in sample WK.Phylogenetic analysis revealed that the bacteria in the two samples fell into four putative divisions,which were Nitrospira,Alphaproteobacteria,Gamaproteobacteria,and Acidobacteria.Organisms of genuses Acidithiobacillus and Leptospirillum,which were in gamaproteobacteria class and Nitrospira family,were dominant in two samples,respectively.In sample TS,which was characterized by low pH,high sulfate,high iron,and high arsenide,two species(Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans)constituted 98.22% of the entire microbial community.Compared with sample TS,the microbial community in sample WK was more diversified according to the observation.Interestedly,the Legionella species,which was rarely observed in the low-pH environment,was detected in sample WK.This work helps us to further understand the diversity of microbial community living in extreme acid mine drainages with unique geochemistry and the tolerance capability of acidophiles to heavy metal. 展开更多
关键词 microbial community acid mine drainage(AMD) tolerance capability PCR-based cloning approach
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Iron Phosphate Coating: A Novel Approach to Controlling Pyrite Oxidation 被引量:2
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作者 HUANGXIAO V.P.EVANGELOU 《Pedosphere》 SCIE CAS CSCD 1997年第2期103-110,共8页
A novel coating technique was developed for controlling Pyrite oxidation. The technique invo1ved leachingpyrite particles with a solution containing low concentrations of phosphate and hydrogen peroxide. Duringthe lea... A novel coating technique was developed for controlling Pyrite oxidation. The technique invo1ved leachingpyrite particles with a solution containing low concentrations of phosphate and hydrogen peroxide. Duringthe leaching process, the iron released from pyrite by hydrogen proxide was precipitated by phosphate as aferric phosphate coating. This coating was shown to be able to effectively prevent Pyrite from oxidation and itcould be established at the expense of only surface portions of Pyrite. The emergence of this technique couldprovide a unique potential route for abating acid mine drainage and reclaiming sulfide-containing degradedmining land. 展开更多
关键词 acid mine drainage COATING PYRITE
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Bioleaching of chalcocite by mixed microorganisms subjected to mutation 被引量:2
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作者 康健 邱冠周 +3 位作者 高健 王海华 吴学玲 丁建南 《Journal of Central South University》 SCIE EI CAS 2009年第2期218-222,共5页
Mixed microorganisms with elevated activity of chalcocite-leaching were screened by mutation methods. The original microorganisms collected from acid mine drainage of different sites were mixed and then treated with m... Mixed microorganisms with elevated activity of chalcocite-leaching were screened by mutation methods. The original microorganisms collected from acid mine drainage of different sites were mixed and then treated with mutagens NO2^- , diethyl sulfate (DES), UV and their combinations, respectively. Five groups of mixed microorganisms with much stronger ore-leaching ability were obtained by screening on the leaching media. Among them, group E of mixed microorganisms (treated with 1% DES for 60 min) with the best perfonnance on chalcocite-leaching, increases the content of Cu^2+ by 101.4% in 20 d of leaching compared with the control culture. In addition, group E is more tolerant to Cu^2+ in media than the control without mutation treatment. Analysis for the diversity of microbial clones indicates that half of operational taxonomic units (OTUs) in group E are Acidithiobacillus ferrooxidans. These observations suggest that group E might have potentials for industrial application. 展开更多
关键词 CHALCOCITE mixed microorganisms BIOLEACHING MUTATION
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Erosion characteristic of slope sandstone soaking in acid mine drainage 被引量:3
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作者 姜立春 陈嘉生 吴爱祥 《Journal of Central South University of Technology》 EI 2007年第2期236-242,共7页
Acid mine drainage(AMD) is one of the main reasons of slope instability in chemical mines with high sulfide. The pH values of the solution inside the mining pit decrease with the increasing of distance from ore body... Acid mine drainage(AMD) is one of the main reasons of slope instability in chemical mines with high sulfide. The pH values of the solution inside the mining pit decrease with the increasing of distance from ore body and vary from 1.2 to 4.6, according to the results of the water environmental investigation and the composition test of the slope sandstone in Xinqiao Pyrite Mine. Comparative experiments between original sandstone and AMD eroded sandstone samples show that after AMD erosion the uniaxial compressive strength and elastic modulus decrease by 30%-50% and 25%-45%, respectively, the cohesion and internal friction angle decrease obviously, and the Poisson ratio fluctuates between 0.20-0.29. The greater joints development, the higher residual stress after peak value, and the longer time to damage. Besides above, the reaction mechanism analysis of AMD eroded sandstone shows that the fillings in joints and fissures of sandstone are frequently decomposed and polyreacted, resulting in changes of interior molecule structure and fi'amework composition, and decreases of cohesion and angle of internal friction between rock structure interfaces. 展开更多
关键词 acid mine drainage SLOPE microstructure SANDSTONE
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Effect of loess for preventing contamination of acid mine drainage from coal waste 被引量:2
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作者 MA Bao-guo WANG Hui-yong +1 位作者 GAO Ran LI Shu-li 《Journal of Coal Science & Engineering(China)》 2012年第3期302-306,共5页
Acid mine drainage (AMD) that releases highly acidic, sulfate and metals-rich drainage is a serious environmental problem in coal mining areas in China. In order to study the effect of using loess for preventing AMD... Acid mine drainage (AMD) that releases highly acidic, sulfate and metals-rich drainage is a serious environmental problem in coal mining areas in China. In order to study the effect of using loess for preventing AMD and controlling heavy metals contamination from coal waste, the column leaching tests were conducted. The results come from experiment data analyses show that the loess can effectively immobilize cadmium, copper, iron, lead and zinc in AMD from coal waste, increase pH value, and decrease Eh, EC, and 8024- concentrations of AMD from coal waste. The oxidation of sulfide in coal waste is prevented by addition of the loess, which favors the generation and adsorption of the alkalinity, the decrease of the population of Thiobacillusferrooxidans, the heavy metals immobilization by precipitation of sulfide and carbonate through biological sul- fate reduction inside the column, and the halt of the oxidation process of sulfide through iron coating on the surface of sulfide in coal waste. The loess can effectively prevent AMD and heavy metals contamination from coal waste in in-situ treatment systems. 展开更多
关键词 acid coal waste acid mine drainage LOESS heavy metals contamination PREVENTION
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16s rDNA based microbial diversity analysis of eleven acid mine drainages obtained from three Chinese copper mines 被引量:4
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作者 谢建平 蒋宏忱 +3 位作者 刘新星 刘学端 周集中 邱冠周 《Journal of Central South University》 SCIE EI CAS 2011年第6期1930-1939,共10页
Eleven acid mine drainage (AMD) samples were obtained from southeast of China for the analysis of the microbial communities diversity, and the relationship with geochemical variables and spatial distance by using a ... Eleven acid mine drainage (AMD) samples were obtained from southeast of China for the analysis of the microbial communities diversity, and the relationship with geochemical variables and spatial distance by using a culture-independent 16S rDNA gene phylogenetic analysis approach and multivariate analysis respectively. The principle component analysis (PCA) of geochemical variables shows that eleven AMDs can be clustered into two groups, relative high and low metal rich (RHMR and RLMR) AMDs. Total 1691 clone sequences are obtained and the detrended correspondence analysis (DCA) of operational taxonomic units (OTUs) shows that, ~,-Proteobacteria, Acidobacteria, Actinobacteria, Cyanobacteria, Firmicutes and Nitrospirae are dominant species in RHMR AMDs. In contrast, a-Proteobacteria, fl-Proteobacteria, Planctomycetes and Bacteriodetes are dominant species in RLMR AMD. Results also show that high-abundance putative iron-oxidizing and only putative sulfur-oxidizing microorganisms are found in RHMR AMD. Multivariate analysis shows that both geochemical variables (r=0.429 3, P=-0.037 7) and spatial distance (r=0.321 3, P=-0.018 1) are significantly positively correlated with microbial community and pH, Mg, Fe, S, Cu and Ca are key geochemistry factors in shaping microbial community. Variance partitioning analysis shows that geochemical variables and spatial distance can explain most (92%) of the variation. 展开更多
关键词 acid mine drainage community structure 16S rDNA gene geochemical variables spatial distance
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