Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently...Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).展开更多
In this study, moisture content, peroxide and free fatty acid values and microbial counts of 30 Karinyagi samples taken from different retail markets in Afyonkarahisar province have been investigated. The average mois...In this study, moisture content, peroxide and free fatty acid values and microbial counts of 30 Karinyagi samples taken from different retail markets in Afyonkarahisar province have been investigated. The average moisture content of Karinyagi samples was found to be 17.64%. The average peroxide values of the samples was found 2.33 meq O2/kg fat. Free fatty acid values of samples ranged at 2.2-5.2 mg NaOH/g fat and the average acidity value was found to be 3.43 mg NaOH/g fat. Total aerobic mesophilic bacteria, yeast/mould, coliform group and Micrococcus/Staphylococcus counts of Karinyagi samples were 5.59, 5.54, 2.56, 1.24 log CFU/g fat, respectively.展开更多
基金We thank for the funding support from the National Standardization Construction in TCMs of China(No.ZYBZH-Y-HUN-23)National Key Research and Development Projects of China(No.2018YFC1707903)Key Research and Development Projects of Hunan Province(No.2018SK2119).
文摘Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).
文摘In this study, moisture content, peroxide and free fatty acid values and microbial counts of 30 Karinyagi samples taken from different retail markets in Afyonkarahisar province have been investigated. The average moisture content of Karinyagi samples was found to be 17.64%. The average peroxide values of the samples was found 2.33 meq O2/kg fat. Free fatty acid values of samples ranged at 2.2-5.2 mg NaOH/g fat and the average acidity value was found to be 3.43 mg NaOH/g fat. Total aerobic mesophilic bacteria, yeast/mould, coliform group and Micrococcus/Staphylococcus counts of Karinyagi samples were 5.59, 5.54, 2.56, 1.24 log CFU/g fat, respectively.
