采用农杆菌介导的方法将高赖氨酸蛋白基因导入台粳9号幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得2株转基因植株.对这些植株及后代植株进行GUS染色和PCR及PCR Southern b lot检测分析,确定高赖氨酸蛋白基因已整合...采用农杆菌介导的方法将高赖氨酸蛋白基因导入台粳9号幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得2株转基因植株.对这些植株及后代植株进行GUS染色和PCR及PCR Southern b lot检测分析,确定高赖氨酸蛋白基因已整合到台粳9号基因组中,并能稳定遗传表达.经检测,转基因水稻糙米赖氨酸含量为0.349%,比对照提高29.3%;转基因水稻秸秆赖氨酸含量为0.341%,比对照提高8.3%.展开更多
To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic a...To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.展开更多
[目的]构建野桑蚕中肠组织cDNA文库并分离其丝氨酸蛋白酶基因。[方法]利用TaKaRa公司生产的cDNA Library Construction Kit构建了野桑蚕中肠组织的cDNA文库,并采用测序法克隆分析丝氨酸蛋白酶基因cDNA。[结果]经鉴定,文库的滴度达6.2...[目的]构建野桑蚕中肠组织cDNA文库并分离其丝氨酸蛋白酶基因。[方法]利用TaKaRa公司生产的cDNA Library Construction Kit构建了野桑蚕中肠组织的cDNA文库,并采用测序法克隆分析丝氨酸蛋白酶基因cDNA。[结果]经鉴定,文库的滴度达6.2×105pfu/ml,文库插入片段的平均大小为1.2 kb左右。从文库的测序结果中获得了野桑蚕丝氨酸蛋白酶基因片段(登录号:EU672968),序列分析结果显示:该基因片段由854个核苷酸组成,编码284个氨基酸残基。通过对该基因片段编码的氨基酸和其他10种昆虫的丝氨酸蛋白酶基因编码的氨基酸的同源性分析,发现该氨基酸序列与其他丝氨酸蛋白酶具有一定的同源性。[结论]该基因的发现对于研究家蚕和野桑蚕对外来入侵物的抗性具有重要意义。展开更多
文摘采用农杆菌介导的方法将高赖氨酸蛋白基因导入台粳9号幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得2株转基因植株.对这些植株及后代植株进行GUS染色和PCR及PCR Southern b lot检测分析,确定高赖氨酸蛋白基因已整合到台粳9号基因组中,并能稳定遗传表达.经检测,转基因水稻糙米赖氨酸含量为0.349%,比对照提高29.3%;转基因水稻秸秆赖氨酸含量为0.341%,比对照提高8.3%.
文摘To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.
文摘[目的]构建野桑蚕中肠组织cDNA文库并分离其丝氨酸蛋白酶基因。[方法]利用TaKaRa公司生产的cDNA Library Construction Kit构建了野桑蚕中肠组织的cDNA文库,并采用测序法克隆分析丝氨酸蛋白酶基因cDNA。[结果]经鉴定,文库的滴度达6.2×105pfu/ml,文库插入片段的平均大小为1.2 kb左右。从文库的测序结果中获得了野桑蚕丝氨酸蛋白酶基因片段(登录号:EU672968),序列分析结果显示:该基因片段由854个核苷酸组成,编码284个氨基酸残基。通过对该基因片段编码的氨基酸和其他10种昆虫的丝氨酸蛋白酶基因编码的氨基酸的同源性分析,发现该氨基酸序列与其他丝氨酸蛋白酶具有一定的同源性。[结论]该基因的发现对于研究家蚕和野桑蚕对外来入侵物的抗性具有重要意义。
