Uridine diphosphate-glucose dehydrogenase （UGD, EC1.1.1 glucuronate）, a critical precursor of cell wall polysaccharides 22 oxidizes UDP-Glc （UDP-D-glucose） to UDP-GlcA （UDP-D- GbUGD6 from Gossypium barbadense is ...Uridine diphosphate-glucose dehydrogenase （UGD, EC1.1.1 glucuronate）, a critical precursor of cell wall polysaccharides 22 oxidizes UDP-Glc （UDP-D-glucose） to UDP-GlcA （UDP-D- GbUGD6 from Gossypium barbadense is more highly expressed late in the elongation of cotton fibers （15 d post-anthesis （DPA）） and during the stage of secondary cell wall thickening （30 DPA）. Subcellular localization analysis in onion epidermis revealed that fluorescently labeled GbUGD6 protein was distribut- ed throughout the cell membrane, as well as the nucleus and vacuoles. Examination of UGD function in Arabidopsis revealed that the antisense GbUGD6 lines had shorter roots, deferred blossoming, compared to wild-type plants. Activities of associated enzymes were also affected by UGD reduction, and biochemical analysis of cell wall samples showed an increase in cellulose levels and a decrease in UGP-GlcA contents. The results of the present study as well as previous studies on UGD support the conclusion that UGD plays a major role in synthesizing polysaccharides synthesis in the cell wall.展开更多
基金the Ministry of Agriculture of China (2014ZX08009-003)the Hebei Province Technology Support Program (14962905D)the Hebei Province Department of Education Fund (Y2012025)
文摘Uridine diphosphate-glucose dehydrogenase （UGD, EC1.1.1 glucuronate）, a critical precursor of cell wall polysaccharides 22 oxidizes UDP-Glc （UDP-D-glucose） to UDP-GlcA （UDP-D- GbUGD6 from Gossypium barbadense is more highly expressed late in the elongation of cotton fibers （15 d post-anthesis （DPA）） and during the stage of secondary cell wall thickening （30 DPA）. Subcellular localization analysis in onion epidermis revealed that fluorescently labeled GbUGD6 protein was distribut- ed throughout the cell membrane, as well as the nucleus and vacuoles. Examination of UGD function in Arabidopsis revealed that the antisense GbUGD6 lines had shorter roots, deferred blossoming, compared to wild-type plants. Activities of associated enzymes were also affected by UGD reduction, and biochemical analysis of cell wall samples showed an increase in cellulose levels and a decrease in UGP-GlcA contents. The results of the present study as well as previous studies on UGD support the conclusion that UGD plays a major role in synthesizing polysaccharides synthesis in the cell wall.
