Three pod lengths （ 10 cm, 10–20 cm and 20 cm） and 13 pre-sowing treatments were considered with four dates of collection （9 February, 19 February, 1 March and 11 March） to investigate their effect on germination...Three pod lengths （ 10 cm, 10–20 cm and 20 cm） and 13 pre-sowing treatments were considered with four dates of collection （9 February, 19 February, 1 March and 11 March） to investigate their effect on germination rate and growth of seedlings of Albizia lebbeck （L.） Benth. at Pundibari, West Bengal, India. The best time of pod collection was on 19 February as the germination rate of the seeds collected on this date was 92.5% without requiring any pre-sowing treatments. This clearly indicated that the seeds collected on 19 February were non-dormant, viable and physiologically mature. Significantly better seedling growth was also obtained when the seeds were sown on 19 February which was extracted from pods of length 10-20 cm. Application of thio-urea as a pre-treatment did not significantly enhanced seed germination rate, but its application in lower concentration （100 μL·L-1） resulted in better seedling growth and development.展开更多
The dried leaves of Apocynum venetum L. （AVL） is a traditional Chinese medicine widely used as antihypertensive medicine in China. Accurate determination of its best harvest season is important for its effective and...The dried leaves of Apocynum venetum L. （AVL） is a traditional Chinese medicine widely used as antihypertensive medicine in China. Accurate determination of its best harvest season is important for its effective and safe use. In the present study, we developed a reliable method based on high-performance liquid chromatography-diode array detector-electro spray ionization-ion trap-time of flight （HPLC-DAD-ESI-IT-TOF） mass spectrometry and HPLC-DAD for the identification and quantification of major components in AVL leaves. Quantitative analysis of 24 samples collected weekly helped monitor the changes of compounds dynamically in AVL leaves to determine the best harvest season. A total of 30 compounds were identified, including quinic acid, five phenolic acids and 24 flavonoids. For the first time, 16 compounds were selected as marker compounds and simultaneously monitored weekly instead of monthly during the growth of the plant. The results showed that in May the leaves had the highest amount of phenolic acids, flavonoids and total compounds. Therefore, May should be the best harvest season for AVL leaves, which was distinct from previous studies. The established method was validated to be simple, accurate and precise, and thus it was of great importance for determination of the best harvest season.展开更多
文摘Three pod lengths （ 10 cm, 10–20 cm and 20 cm） and 13 pre-sowing treatments were considered with four dates of collection （9 February, 19 February, 1 March and 11 March） to investigate their effect on germination rate and growth of seedlings of Albizia lebbeck （L.） Benth. at Pundibari, West Bengal, India. The best time of pod collection was on 19 February as the germination rate of the seeds collected on this date was 92.5% without requiring any pre-sowing treatments. This clearly indicated that the seeds collected on 19 February were non-dormant, viable and physiologically mature. Significantly better seedling growth was also obtained when the seeds were sown on 19 February which was extracted from pods of length 10-20 cm. Application of thio-urea as a pre-treatment did not significantly enhanced seed germination rate, but its application in lower concentration （100 μL·L-1） resulted in better seedling growth and development.
基金Initiative Drug Discovery of National Science and Technology Major Project(Grant No.2014ZX09304307-001-011)
文摘The dried leaves of Apocynum venetum L. （AVL） is a traditional Chinese medicine widely used as antihypertensive medicine in China. Accurate determination of its best harvest season is important for its effective and safe use. In the present study, we developed a reliable method based on high-performance liquid chromatography-diode array detector-electro spray ionization-ion trap-time of flight （HPLC-DAD-ESI-IT-TOF） mass spectrometry and HPLC-DAD for the identification and quantification of major components in AVL leaves. Quantitative analysis of 24 samples collected weekly helped monitor the changes of compounds dynamically in AVL leaves to determine the best harvest season. A total of 30 compounds were identified, including quinic acid, five phenolic acids and 24 flavonoids. For the first time, 16 compounds were selected as marker compounds and simultaneously monitored weekly instead of monthly during the growth of the plant. The results showed that in May the leaves had the highest amount of phenolic acids, flavonoids and total compounds. Therefore, May should be the best harvest season for AVL leaves, which was distinct from previous studies. The established method was validated to be simple, accurate and precise, and thus it was of great importance for determination of the best harvest season.
