ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae （Mhp） in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentr...ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae （Mhp） in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentration aerosol sampler was designed for collecting Mhp aerosol. Firstly, the collection was performed in a closed environment full of artificial aerosol of Mhp. Secondly, collection efficiency was detected by real-time PCR. Thereafter, the clinical feasibility of the designed equipment was tested by collecting aerosol samples in different pig herds. In one assay, the samples were collected at different times from one pig house challenged with Mhp. In another assay, the samples was collected from the delivery room, nursery and fattenning house of a MPS outbreak farm as well as a Mhp infection positive pig farm without obvious clinical symptoms. All the aerosol samples were then detected by real-time PCR or nested PCR. ResultThe collection efficiency of the designed bioaerosol sampler was （37.04±6.43） %, Mhp could be detected 7 d after intratracheal challenge with pneumonic lung homogenate suspension. Aerosol samples of 11 pig houses from the two Mhp positive pig farms with or without clinical symptoms all showed a positive result of PCR, the positivity rate was 100%. ConclusionA high sensitive collecting and detecting technology of aerosol was successfully established, which can be applied to clinical detection of Mhp in aerosol.展开更多
The high resolution Terra SAR-X dataset was employed with DIn SAR and persistent scatterer interferometry（PSI） technique for subsidence monitoring in a mountainous area. For DInS AR technique, the generally used SRT...The high resolution Terra SAR-X dataset was employed with DIn SAR and persistent scatterer interferometry（PSI） technique for subsidence monitoring in a mountainous area. For DInS AR technique, the generally used SRTM and relief-DEM, which was derived from aerial topographic map, were used to evaluate the influence of external DEM. The results show that SRTM could not fully compensate the complex topography of the research area. The corner reflectors installed during the acquisition of SAR dataset were used to estimate the accuracy of geocoding. The terrain corrected geocoding results based on relief-DEM were much better than using SRTM, with the root mean square error（RMSE） being 6.35 m in X direction and 11.65 m in Y direction（both in UTM projection）, around one pixel of the multilooked intensity image to be geocoded. For PSI technique, the results from time-series analysis of multi-baseline differential interferograms were integrated to restrict only persistent scatterer candidates near the boundary of subsiding area for regression analysis. The results demonstrate that PSI can refine the boundary of subsidence, which could then be used to derive some angular parameters to help people to learn the law of subsidence caused by repeated excavation in this area.展开更多
According to the effective sampling of particles and the particles impoverishment caused by re-sampling in particle filter,an improved particle filtering algorithm based on observation inversion optimal sampling was p...According to the effective sampling of particles and the particles impoverishment caused by re-sampling in particle filter,an improved particle filtering algorithm based on observation inversion optimal sampling was proposed. Firstly,virtual observations were generated from the latest observation,and two sampling strategies were presented. Then,the previous time particles were sampled by utilizing the function inversion relationship between observation and system state. Finally,the current time particles were generated on the basis of the previous time particles and the system one-step state transition model. By the above method,sampling particles can make full use of the latest observation information and the priori modeling information,so that they further approximate the true state. The theoretical analysis and experimental results show that the new algorithm filtering accuracy and real-time outperform obviously the standard particle filter,the extended Kalman particle filter and the unscented particle filter.展开更多
The variation of the radiation field around accelerator is complex.Only by real-time monitoring can one learn them in detail.The traditional measurement methods have been out of date.The mini-DDL is a specific device ...The variation of the radiation field around accelerator is complex.Only by real-time monitoring can one learn them in detail.The traditional measurement methods have been out of date.The mini-DDL is a specific device designed for such radiation field real-time monitoring.It has huge flash memories and is small in size.It can communicate with the control PC via intranet or RS232 port.So it is suitable to be applied not only in area monitoring but also in temporary monitoring.The radiation field measurements results obtained using the Mini-DDL are introduced in this paper.展开更多
Over the past decades, low-energy electron accelerators have been used worldwide for surface curing and sterilization. The beam nonuniformity is an important parameter of the low-energy electron beam with large cross-...Over the past decades, low-energy electron accelerators have been used worldwide for surface curing and sterilization. The beam nonuniformity is an important parameter of the low-energy electron beam with large cross-sections. A simple and accurate measurement system of nonuniformity for the low-energy electron beam with large cross-sections was developed. The main concept consists in the measurement of nonuniformity, which is realized by using a linear actuator to drive two scanning wires through the beam's cross-sections at a fixed speed. The beam distribution can be obtained by sending/collecting the current signals to/from the Data Acquisition (DAQ) software on a laptop by a USB DAQ card. This device is very convenient for the performance testing of a new accelerator at the manufacturer's site. The distribution of the homemade low voltage electron accelerator EBS-300-50 was measured and evaluated.展开更多
基金Supported by the Fund for Agricultural Science and Technology Independent Innovation of Jiangsu Province[CX(12)1001-05]~~
文摘ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae （Mhp） in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentration aerosol sampler was designed for collecting Mhp aerosol. Firstly, the collection was performed in a closed environment full of artificial aerosol of Mhp. Secondly, collection efficiency was detected by real-time PCR. Thereafter, the clinical feasibility of the designed equipment was tested by collecting aerosol samples in different pig herds. In one assay, the samples were collected at different times from one pig house challenged with Mhp. In another assay, the samples was collected from the delivery room, nursery and fattenning house of a MPS outbreak farm as well as a Mhp infection positive pig farm without obvious clinical symptoms. All the aerosol samples were then detected by real-time PCR or nested PCR. ResultThe collection efficiency of the designed bioaerosol sampler was （37.04±6.43） %, Mhp could be detected 7 d after intratracheal challenge with pneumonic lung homogenate suspension. Aerosol samples of 11 pig houses from the two Mhp positive pig farms with or without clinical symptoms all showed a positive result of PCR, the positivity rate was 100%. ConclusionA high sensitive collecting and detecting technology of aerosol was successfully established, which can be applied to clinical detection of Mhp in aerosol.
基金Project(51174191)supported by the National Natural Science Foundation of ChinaProject(2013CB227904)supported by the National Basic Research Program of ChinaProject(2012QNB09)supported by Fundamental Research Funds for the Central Universities,China
文摘The high resolution Terra SAR-X dataset was employed with DIn SAR and persistent scatterer interferometry（PSI） technique for subsidence monitoring in a mountainous area. For DInS AR technique, the generally used SRTM and relief-DEM, which was derived from aerial topographic map, were used to evaluate the influence of external DEM. The results show that SRTM could not fully compensate the complex topography of the research area. The corner reflectors installed during the acquisition of SAR dataset were used to estimate the accuracy of geocoding. The terrain corrected geocoding results based on relief-DEM were much better than using SRTM, with the root mean square error（RMSE） being 6.35 m in X direction and 11.65 m in Y direction（both in UTM projection）, around one pixel of the multilooked intensity image to be geocoded. For PSI technique, the results from time-series analysis of multi-baseline differential interferograms were integrated to restrict only persistent scatterer candidates near the boundary of subsiding area for regression analysis. The results demonstrate that PSI can refine the boundary of subsidence, which could then be used to derive some angular parameters to help people to learn the law of subsidence caused by repeated excavation in this area.
基金Project(60634030) supported by the Key Project of the National Natural Science Foundation of ChinaProject(60702066) supported by the National Natural Science Foundation of China+1 种基金Project (2007ZC53037) supported by Aviation Science Foundation of ChinaProject(CASC0214) supported by the Space-Flight Innovation Foundation of China
文摘According to the effective sampling of particles and the particles impoverishment caused by re-sampling in particle filter,an improved particle filtering algorithm based on observation inversion optimal sampling was proposed. Firstly,virtual observations were generated from the latest observation,and two sampling strategies were presented. Then,the previous time particles were sampled by utilizing the function inversion relationship between observation and system state. Finally,the current time particles were generated on the basis of the previous time particles and the system one-step state transition model. By the above method,sampling particles can make full use of the latest observation information and the priori modeling information,so that they further approximate the true state. The theoretical analysis and experimental results show that the new algorithm filtering accuracy and real-time outperform obviously the standard particle filter,the extended Kalman particle filter and the unscented particle filter.
文摘The variation of the radiation field around accelerator is complex.Only by real-time monitoring can one learn them in detail.The traditional measurement methods have been out of date.The mini-DDL is a specific device designed for such radiation field real-time monitoring.It has huge flash memories and is small in size.It can communicate with the control PC via intranet or RS232 port.So it is suitable to be applied not only in area monitoring but also in temporary monitoring.The radiation field measurements results obtained using the Mini-DDL are introduced in this paper.
文摘Over the past decades, low-energy electron accelerators have been used worldwide for surface curing and sterilization. The beam nonuniformity is an important parameter of the low-energy electron beam with large cross-sections. A simple and accurate measurement system of nonuniformity for the low-energy electron beam with large cross-sections was developed. The main concept consists in the measurement of nonuniformity, which is realized by using a linear actuator to drive two scanning wires through the beam's cross-sections at a fixed speed. The beam distribution can be obtained by sending/collecting the current signals to/from the Data Acquisition (DAQ) software on a laptop by a USB DAQ card. This device is very convenient for the performance testing of a new accelerator at the manufacturer's site. The distribution of the homemade low voltage electron accelerator EBS-300-50 was measured and evaluated.
