诬告行为的释因逻辑及其治理路径研究

诬告行为是以匿名举报为主要形式,捏造虚假事实,意图让被诬告者遭受违纪处分甚至刑事追究的行为,与因情况了解不全面或认识偏差而导致的错告、检举失实行为有本质区别。诬告行为可能对被诬告者的生活和精神造成一定的损害,同时也浪费公共资源,扰乱党政机关正常工作秩序。诬告行为的产生是监督权利的异化,在一定程度上也反映出党内政治生态的不平衡。研究发现,诬告者可能因外界刺激引发自身内心的不满,借助于尚不完善的制度体系,实施诬告这一恶化政治生态的行为。完善举报制度,正确行使监督权利,提倡实名举报是减少诬告行为的有效对策;建立澄清保护机制,健全责任追究制度是维护被诬告者的重要举措。

《思想道德修养与法律基础》教学困境释因与突破——以人性论为视角

枯燥乏味是当下困扰《思想道德修养与法律基础》教学的普遍困境,原因很多,但问题的根源似宜从人性视角进行挖掘。从人性的欲望、情感、理智、精神和理性五层次分析成因,有助于洞悉枯燥心理产生的深层秘密。在此基础上指出实践是突破枯燥困境的根本途径。对此,从必然性角度看,精神现象具有超越性、抽象性两大特点,它们造成对精神性知识接受的障碍,这个障碍只有依赖实践方能突破;从可能性角度看,大学生具有参与意识强的特点,这为开展实践教学提供了可能性。

重动句的口头释因格式探析

重动句(动词拷贝结构)有一种简省形式,即"V+O+V+的"结构格式,在口语交际中主要用来解释事情的原因。"V+O+V+的"所对应的完整形式是致使性重动句,它们都是主观过量构式。与致使性重动句不同,这种口头释因格式结构简短而凝固,意义焦点明确,所适用语境主要为答话或叙述的后续句。这种格式的使用符合会话的"合作原则"及"礼貌原则"。另外,"V+O+V+的"格式对"V+O"存在值得注意的选择倾向:容易导致非预期结果的行为更易进入"V+O+V+的"格式。

市场分割的制度性质及其对经济增长的释因研究

本文介绍了市场分割的定义、测度方法以及成因,然后阐述中国及中国部分区域市场分割现状,针对中国各个区域市场分割的研究表明,中国的市场分割程度总体上趋于下降的态势。文章还就市场分割对经济增长的影响进行分析,对相关领域的研究有一定的借鉴作用。

释因连接成分“主要是”及其词汇化倾向

"主要是"作为一个跨段结构,有词汇化的倾向,逐渐凝固成为一个释因连接成分。本文首先描写跨段结构"主要是"和词汇化性质的"主要是"二者的语义特点,在此基础上讨论两者之间的关系以及影响其词汇化的因素。

近代汉语“释因”类语用标记及其演变

'释因'类语用标记是指引出事件原因或原委的语言表达式。引出事件原因的称为'原因标记',如'是因为';引出事件原委的称为'原委标记',如'是这么回事'。文章对近代汉语的释因标记进行了分类描写,并讨论了它们向现代演变的问题。

“谁让+NP+VP”的构式化及其表现

本文在分析现代汉语“谁让+NP+VP”结构形式与意义(功能)对应关系的基础上,追溯“谁让+NP+VP”从表示真性询问到表示反问或否定,再到起释因作用的语义演变历程及其构式化历程和表现。文章认为,“NP”“NP+VP”的指谓对象的变化是相关演变的重要诱因。

由“妄议中央”说开去

近来"妄议中央"这一动宾短语掀起全民讨论浪潮。通过介绍"妄议中央"这一短语的源起与发展,以共时角度把握这一短语的争议核心,根据辞书释义解析该短语存在争议的原因,最后以最近新闻跟踪来窥探这一方针政策的核心要义,以期让读者更全面认识党中央出台的这一政策。

C-terminal 76 Amino Acids of eRF3 Are Not Required for the Binding of Release Factor eRF1a from Euplotes octocarinatus

Termination of translation in eukaryotes requires two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, whereas eRF3 is a ribosome-dependent and eRFl-dependent GTPase. Polypeptide release factor eRF3 consists of N-terminal variable region and C-terminal conserved part. C-terminal part of eRF3 is responsible for termination of the translation, In the present study, the C-terminal of Euplotes octocarinatus eRF3 （eRF3C） and truncate eRF3C lacking 76 amino acids in C-terminal （eRF3Ct） were expressed in Escherichia coll. The recombinant GST-eRF3C and GST-eRF3Ct polypeptides were purifled by affinity chromatography using glutathione Sepharose 4B column. After enzymatic cleavage of GST tail, the eRF3C and eRF3Ct protein were obtained. Pull-down analysis showed that the recombinant GST-eRF3C and GST-eRF3Ct polypeptides interacted with E. octocarinatus polypeptide chain release factor eRF1a. This result suggested that the C-terminal of eRF3 having 76 amino acids were not required for the binding of eRFla in Euplotes octocarinatus.

Anti-Plane Elasticity Problem and Mode Ⅲ Crack Problem of Cubic Quasicrystal

The fracture theory of cubic quasicrystal was developed. The exact analytic solution of a Mode Ⅲ Griffith crack in the material was obtained by using the Fourier transform and dual integral equations theory, and so the displacement and stress fields, the stress intensity factor and strain energy release rate were determined. The results show that the stress intensity factor is independent of material constants, and the strain energy release rate is dependent on all material constants. These provide important information for studying the deformation and fracture of the new solid material.

话语标记“是这样(的)”的特征功能与形成机制

本文从功能主义角度讨论话语标记"是这样(的)"的使用条件和句法语义特征,着重分析话语标记"是这样"的功能、机制与动因。本文的讨论显示,"是这样"的特征及其与句法语义条件的互动,使得话语标记"是这样"具有"传我所知""告你不知"和"据情推知"的告知功能,演变过程中"是这样"由表达命题意义、参与句法成分的建构变为了明示焦点信息、标记告知功能的话语标记。

Study of Sustained Release Phenylpropanolamine Hydrochloride Hydrophilic Matrix Tablets Containing Hydroxypropylmethylcellulose K100M and Carbopol 971P

选用HPMC K100M和卡波普971P为骨架材料，粉末直接压片法制备骨架片，考察释放度，反相高效液相色谱法检测盐酸苯丙醇胺（PPA·HCl）的浓度。释药曲线均符合Higuchi方程（R＞0．98，P＜0．01）。运用正交设计法，以美国市场的PPA·HCl缓释片Acutrim^R为对照，相似因子f2值为指标，筛选获得了最优处方。其工艺重现性合格。研制片在0．1mol·L^-1 HCl,H2O(pH6.5)，磷酸盐缓冲液（PBS）pH5.0,6.8和7.4的介质中，以及在0.1mol·L^-1HCl中释放2h，转移至PBS6．8中释放10h，相对于对照品的f2值为63－74，表明在各介质中两制剂的释药曲线相似。释药影响因素的考察结果表明：在本实验考察的范围内，骨架片在水中的释药速率与HPMC K100M和卡波普971P的用量呈负相关。HPMC K100M和卡波普971P的比例（保持聚合物总用量相同），硬脂酸镁量和骨架片硬度对释药速率无显著性影响。

Fixed-Tumor Vaccine: A Practical Formulation with Cytokine-Microspheres for Protective and Therapeutic Antitumor Immunity

Objective: To study the protective and therapeutic antitumor immunity against hepatocellular carcinoma (HCC) with the fixed-tumor vaccine.Methods: A tumor vaccine consisting of fixed tumor cells or fixed tumor fragments combined with sustained-releasers of cytokines and a non-toxic adjuvant was developed. C57BL/6J mice were immunized intra-dermally with the vaccine on day 0 and 7, followed by intrahepatic challenge with live Hepa 1–6 cells.Results: All of 15 nonimmunized control mice developed the hepatoma. Protection of mice immunized with fixed Hepa 1–6 cells and both of IL-2/GM-CSF microspheres or further mixed with TiterMax Gold reached 80% and 87%, respectively. Mass growth of the established tumors, vaccinated twice at 5 mm in diameter, the tumor of control animals continued to grow. However, 7–10 days after the second injection of the tumor vaccine, the tumor growth was suppressed in 9 of 10 mice and then markedly reduced. Complete tumor regression was observed in 60% (6/10) of mice. Splenocytes from the control mice were not able to lyse target Hepa 1–6 cells and other tumor cells. In contrast splenocytes from the vaccinated mice exhibited a 41% lytic activity against the Hepa 1–6 cells tested at an effector/target (E/T) ratio of 5, whereas they did not exhibited such activity against the melanoma cells (B16-F1), Lewis lung carcinoma cells (LLC), renal carcinoma cells (Renca), and bladder carcinoma cells (MBT-2). The cytotoxic activity was inhibited by the treatment with anti-CD3, anti-CD8, and anti-MHC-class I monoclonal antibodies but not with anti-CD4 and anti-MHC-class II antibodies. In the Phase-I clinical trial, vaccination of HCC patients with the autologous vaccine is a well-tolerated treatment and induces fixed tumor fragment-specific immunity.Conclusion: Fixed HCC vaccination elicited protective and therapeutic antitumor immunity against HCC. The tumor vaccine elicited antigen specific CTL response lysis of the target HCC was mediated by the typical MHC-class I restricted CD8+ T cells. Key words cancer vaccine - cytotoxic T lymphocyte - immunotherapy - hepatoma

Release Characteristics of Release-controlled Nitrogen Fertilizer in Winter Wheat on Dry Land

[Objective] The aim was to study the release characteristics of different release-controlled fertilizers, as well as their effects on wheat growth and develop- ment under water stress. [method] With phosphorus-potassium fertilization and opti- mized fertilization （OPT） as the control, the release characteristics of 4 kinds of re- lease-controlled nitrogen fertilizers （A, B, C and D） coated with different materials were studied. [Result] Under the same phosphorus and potassium levels, the re- lease-controlled nitrogen fertilizers A and B significantly improved the yield of wheat. Spike number is the main reason leading to the difference in yield. The fertilizers A and B were released by 44.4% and 46.3% before winter, released by 72.1% and 69.8% at the jointing stage, and related by 88.0% and 91.5% in the harvest period, meeting the nitrogen requirement of dryland wheat across the growth period. Com- pared with OPT, the nitrogen accumulation amounts in treatments A and B were increased by 1.39 and 2.09 kg/667 m2, the nitrogen use efficiencies were increased by 8.66% and 13.04%, and the nitrogen partial factor productivities were increased by 9.00 and 7.22 kg/kg, respectively. [Conclusion] Among the 4 kinds of fertilizers, A and B were considered as the optimum release-controlled nitrogen fertilizers for winter wheat on dry land.

Cloning and Expression of Gonadotrophin Releasing Hormone Receptor in Apis cerana cerana

[Objective] This study was to clone the GnRH （gonadotropin-releasing hormone）, and to investigate its expression in Apis cerana cerana. [Method] The cDNA sequence of GnRHR gene was amplified from Apis cerana cerana by using RT-PCR techniques. It was conducted with bioinformatics analysis and the in situ hybridization histochemistry of its expression products was studied. [Result] The sequence analy- sis showed that the full cDNA sequence was 1 050 bp with the open reading frame of 1 050 bp, and it encoded 349 amino acid residues. The deduced amino sequence included 7 transmembrane regions, and the predicted molecular mass and isoelectric point were 40.6 kD and 9.54, respectively. The cluster analysis showed that the GnRHR from ＇,4. cerana cerana had close relationship to the GnRHR II from other insects. In situ hybridization showed that Bee-GnRHR staining was specifically localized to the brain, intestine, fat body and testis. [Conclusion] The results indicated that the GnRHR provided molecular bond for the reproduction and metabolism for insects, and suggested a functional role for bee-GnRHR signaling in the coupling of reproduction activities and environment conditions.

Attenuated adenomatous polyposis of the large bowel: Present and future

Attenuated adenomatous polyposis(AAP) is a poorly understood syndrome, that can be defined as the presence of 10-99 synchronous adenomas in the large bowel, and it is considered a phenotypic variant of familial adenomatous polyposis(FAP). This definition has the advantage of simplicity, but it may include sporadic multiple adenomas of the large bowel at an extreme, or FAP cases on the other side. AAP shows a milder phenotype than FAP, with an older age of onset of adenomas and cancer, and less frequent extracolonic manifestations. AAP may be diagnosed as a single case in a family or, less frequently, it may be present in other family members, and it shows distinct pattern of inheritance. In less than 50% of cases, it may be caused by adenomatous polyposis coli(APC) or MUTYH mutations, referred to as APC-associated polyposis, inherited as an autosomal dominant trait, or MUTYH-associated polyposis, which shows an autosomal recessive mechanism of inheritance, respectively. Surveillance should rely on colonoscopy at regular intervals, with removal of adenomas and careful histological examination. When removal of polyps is not possible or advanced lesions are observed, the surgical approach is mandatory, being subtotal colectomy with ileo-rectal anastomosis the treatment of choice. Studies on this syndrome are lacking, and controversies are still present on many issues, thus, other clinical and genetic studies are requested.

Involvement of CRF2 signaling in enterocyte differentiation

To determine the role of corticotropin releasing factor receptor (CRF2) in epithelial permeability and enterocyte cell differentiation.METHODSFor this purpose, we used rat Sprague Dawley and various colon carcinoma cell lines (SW620, HCT8R, HT-29 and Caco-2 cell lines). Expression of CRF2 protein was analyzed by fluorescent immunolabeling in normal rat colon and then by western blot in dissociated colonic epithelial cells and in the lysates of colon carcinoma cell lines or during the early differentiation of HT-29 cells (ten first days). To assess the impact of CRF2 signaling on colonic cell differentiation, HT-29 and Caco-2 cells were exposed to Urocortin 3 recombinant proteins (Ucn3, 100 nmol/L). In some experiments, cells were pre-exposed to the astressin 2b (A2b) a CRF2 antagonist in order to inhibit the action of Ucn3. Intestinal cell differentiation was first analyzed by functional assays: the trans-cellular permeability and the para-cellular permeability were determined by Dextran-FITC intake and measure of the transepithelial electrical resistance respectively. Morphological modifications associated to epithelial dysfunction were analyzed by confocal microscopy after fluorescent labeling of actin (phaloidin-TRITC) and intercellular adhesion proteins such as E-cadherin, p120ctn, occludin and ZO-1. The establishment of mature adherens junctions (AJ) was monitored by following the distribution of AJ proteins in lipid raft fractions, after separation of cell lysates on sucrose gradients. Finally, the mRNA and the protein expression levels of characteristic markers of intestinal epithelial cell (IEC) differentiation such as the transcriptional factor krüppel-like factor 4 (KLF4) or the dipeptidyl peptidase IV (DPPIV) were performed by RT-PCR and western blot respectively. The specific activities of DPPIV and alkaline phosphatase (AP) enzymes were determined by a colorimetric method.RESULTSCRF2 protein is preferentially expressed in undifferentiated epithelial cells from the crypts of colon and in human colon carcinoma cell lines. Furthermore, CRF2 expression is down regulated according to the kinetic of HT-29 cell differentiation. By performing functional assays, we found that Ucn3-induced CRF2 signaling alters both para- and trans-cellular permeability of differentiated HT-29 and Caco-2 cells. These effects are partly mediated by Ucn3-induced morphological changes associated with the disruption of mature AJ in HT-29 cells and tight junctions (TJ) in Caco-2 cells. Ucn3-mediated activation of CRF2 decreases mRNA and protein expression levels of KLF4 a transcription factor involved in IEC differentiation. This signaling is correlated to a down-regulation of key IEC markers such as DPPIV and AP, at both transcriptional and post-transcriptional levels.CONCLUSIONOur findings suggest that CRF2 signaling could modulate IEC differentiation. These mechanisms could be relevant to the stress induced epithelial alterations found in inflammatory bowel diseases.

Alcohol metabolites and lipopolysaccharide: Roles in the development and/or progression of alcoholic liver disease

The onset of alcoholic liver disease (ALD) is initiated by different cell types in the liver and a number of different factors including: products derived from ethanol-induced inflammation, ethanol metabolites, and the indirect reactions from those metabolites. Ethanol oxidation results in the production of metabolites that have been shown to bind and form protein adducts, and to increase inflammatory, fibrotic and cirrhotic responses. Lipopolysaccharide (LPS) has many deleterious effects and plays a significant role in a number of disease processes by increasing inflammatory cytokine release. In ALD, LPS is thought to be derived from a breakdown in the intestinal wall enabling LPS from resident gut bacterial cell walls to leak into the blood stream. The ability of adducts and LPS to independently stimulate the various cells of the liver provides for a two-hit mechanism by which various biological responses are induced and result in liver injury. Therefore, the purpose of this article is to evaluate the effects of a two-hit combination of ethanol metabolites and LPS on the cells of the liver to increase inflamma-tion and fi brosis, and play a role in the development and/or progression of ALD.

Functional characterization of the human-specific （type II） form of kallikrein 8, a gene involved in learning and memory

Kallikrein 8 （KLK8） is a serine protease functioning in the central nervous system, and essential in many aspects of neuronal activities. Sequence comparison and gene expression analysis among diverse primate species identified a human-specific splice form of KLK8 （type II） with preferential expression in the human brain, which may contribute to the origin of human cognition. To gain insights into the physiological and biochemical role of this novel form, we conducted functional analyses of human type II KLK8. Our results show that type II KLK8 is abundantly expressed in human embryonic stem cells and in embryo brain samples, suggesting a potential role in embryogenesis. There are dramatic expression variations in different individuals and brain regions, which is a reflection of its dynamic role in neural activities. Furthermore, the transcription start site （TSS） of KLK8 is tissue-specific, with a brain-specific TSS found in humans indicating functional specialization. Our in vitro biochemical assay shows that there is a type II-specific intermediate protein form, although the processed end-point enzymes are the same for both type 1 and type II KLK8, suggesting that the emergence of type II KLK8 in the human brain likciy leads to functional modifications of KLK8.

Corticotropin-releasing factor secretion from dendritic cells stimulated by commensal bacteria

AIM:To study the production and secretion of corticotropin-releasing factor (CRF) by dendritic cells and the influence of commensal bacteria.METHODS:JAWSⅡ cells (ATCC CRL-11904),a mouse dendritic cell line,were seeded into 24-well culture plates and grown for 3 d.Commensal bacterial strains of Clostridium clostrodiiforme (JCM1291),Bacteroides vulgatus (B.vulgatus) (JCM5856),Escherichia coli (JCM1649),or Fusobacterium varium (F.varium) (ATCC8501) were added to the cells except for the control well,and incubated for 2 h.After incubation,we performed enzyme-linked immunosorbent assay for the cultured medium and reverse transcription polymerase chain reaction for the dendritic cells,and compared these values with controls.RESULTS:The level of CRF secretion by control dendritic cells was 40.4±6.2 pg/mL.The CRF levels for cells incubated with F.varium and B.vulgatus were significantly higher than that of the control (P<0.0001).CRF mRNA was present in the control sample without bacteria,and CRF mRNA levels in all samples treated with bacteria were above that of the control sample.F.varium caused the greatest increase in CRF mRNA expression.CONCLUSION:Our results suggest that dendritic cells produce CRF,a process augmented by commensal bacteria.