Eutrophication or the process of nutrient enrichment of stagnant waters due to excessive use of fertilizer is becoming a critical issue worldwide. Lake Gregory, an artificial lake situated in Nuwara Eliya, Sri Lanka w...Eutrophication or the process of nutrient enrichment of stagnant waters due to excessive use of fertilizer is becoming a critical issue worldwide. Lake Gregory, an artificial lake situated in Nuwara Eliya, Sri Lanka was once a very attractive landscape feature and recreational area attracting a large number of visitors. Rapid urbanization in surrounding areas and the consequent intensification of agricultural and industrial activities led to eutrophication and siltation in the lake. Present study was conducted to detect cyanobacterial diversity and their ability to produce hepatotoxic microcystins using polymerase chain reaction (PCR)-based techniques. Twenty five water samples (surface and bottom) were collected from the lake and total nitrogen and total carbon were estimated. Cyanobacterial cultures were grown in appropriate media and microscopic observations were used to determine the morphological diversity of cyanobacteria isolated from different sites. Genomic DNA was isolated and purified from cyanobacteria using Boom's method. DNA samples were analyzed by PCR with oligonucleotide primers for 16S rRNA gene and mcyA gene of the operon that encodes a microcystin synthetase. The 16S rRNA gene sequences revealed the presences of cyanobacteria belong to Synechococcus sp., Microcystis aeruginosa, Calothrix sp., Leptolyngbya sp., Limnothrix sp., order Oscillatoriales and order Chroococcales. The sequences obtained from this study were deposited in the database under the accession numbers (GenBank: GU368104-GU368116). PCR amplification of mcyA primers indicated the potential for toxin formation of isolated M. aeruginosa from Lake Gregory. This preliminary study shows that the Lake Gregory is under the potential risk of cyanobacterial toxicity. Clearly more work is needed to extend this finding and clarify if other cyanobacterial isolates have genetic potential to produce microcystin since this lake is utilized for recreational activities.展开更多
This paper discusses the first eigenvalue on a compact Riemann manifold with the negative lower bound Ricci curvature. Let M be a compact Riemann manifold with the Ricci curvature≥-R, R=const. ≥0 and d is the diamet...This paper discusses the first eigenvalue on a compact Riemann manifold with the negative lower bound Ricci curvature. Let M be a compact Riemann manifold with the Ricci curvature≥-R, R=const. ≥0 and d is the diameter of M. Our main result is that the first eigenvalue λ1 of M satisfies λ1≥π^2/d^2-0.518R.展开更多
Using unstructured meshes provides great flexibility for modeling the flow in complex geomorphology of tidal creeks,barriers and islands,with refined grid resolution in regions of interest and not elsewhere.In this pa...Using unstructured meshes provides great flexibility for modeling the flow in complex geomorphology of tidal creeks,barriers and islands,with refined grid resolution in regions of interest and not elsewhere.In this paper,an unstructured three-dimensional fully coupled wave-current model is developed.Firstly,a parallel,unstructured wave module is developed.Variations in wave properties are governed by a wave energy equation that includes wave-current interactions and dissipation representative of wave breaking.Then,the existing Finite-Volume Coastal Ocean Model(FVCOM) is modified to couple with the wave module.The couple procedure includes depth dependent wave radiation stress terms,Stokes drift,vertical transfer of wave-generated pressure transfer to the mean momentum equation,wave dissipation as a source term in the turbulence kinetic energy equation,and mean current advection and refraction of wave energy.Several applications are presented to evaluate the developed model.In particular the wind and wave-induced storm surge generated by Hurricane Katrina is investigated.The obtained results have been compared to the in situ measurements with respect to the wave heights and water level elevations revealing good accuracy of the model in reproduction of the investigated events.In a comparison to water level measurements at Dauphin Island,inclusion of the wave induced water level setup reduced the normalized root mean square error from 0.301 to 0.257 m and increased the correlation coefficient from 0.860 to 0.929.Several runs were carried out to analyze the effects of waves.The experiments show that among the processes that represent wave effects,radiation stress and wave-induced surface stress are more important than wave-induced bottom stress in affecting the water level.The Hurricane Katrina simulations showed the importance of the inclusion of the wave effects for the hindcast of the water levels during the storm surge.展开更多
文摘Eutrophication or the process of nutrient enrichment of stagnant waters due to excessive use of fertilizer is becoming a critical issue worldwide. Lake Gregory, an artificial lake situated in Nuwara Eliya, Sri Lanka was once a very attractive landscape feature and recreational area attracting a large number of visitors. Rapid urbanization in surrounding areas and the consequent intensification of agricultural and industrial activities led to eutrophication and siltation in the lake. Present study was conducted to detect cyanobacterial diversity and their ability to produce hepatotoxic microcystins using polymerase chain reaction (PCR)-based techniques. Twenty five water samples (surface and bottom) were collected from the lake and total nitrogen and total carbon were estimated. Cyanobacterial cultures were grown in appropriate media and microscopic observations were used to determine the morphological diversity of cyanobacteria isolated from different sites. Genomic DNA was isolated and purified from cyanobacteria using Boom's method. DNA samples were analyzed by PCR with oligonucleotide primers for 16S rRNA gene and mcyA gene of the operon that encodes a microcystin synthetase. The 16S rRNA gene sequences revealed the presences of cyanobacteria belong to Synechococcus sp., Microcystis aeruginosa, Calothrix sp., Leptolyngbya sp., Limnothrix sp., order Oscillatoriales and order Chroococcales. The sequences obtained from this study were deposited in the database under the accession numbers (GenBank: GU368104-GU368116). PCR amplification of mcyA primers indicated the potential for toxin formation of isolated M. aeruginosa from Lake Gregory. This preliminary study shows that the Lake Gregory is under the potential risk of cyanobacterial toxicity. Clearly more work is needed to extend this finding and clarify if other cyanobacterial isolates have genetic potential to produce microcystin since this lake is utilized for recreational activities.
基金Supported by the NNSF of China(10271011)Supported by LIMB of the Ministry of Education China
文摘This paper discusses the first eigenvalue on a compact Riemann manifold with the negative lower bound Ricci curvature. Let M be a compact Riemann manifold with the Ricci curvature≥-R, R=const. ≥0 and d is the diameter of M. Our main result is that the first eigenvalue λ1 of M satisfies λ1≥π^2/d^2-0.518R.
基金supported by the National Natural Science Foundation of China (Grant Nos.50839001 and 50779006)
文摘Using unstructured meshes provides great flexibility for modeling the flow in complex geomorphology of tidal creeks,barriers and islands,with refined grid resolution in regions of interest and not elsewhere.In this paper,an unstructured three-dimensional fully coupled wave-current model is developed.Firstly,a parallel,unstructured wave module is developed.Variations in wave properties are governed by a wave energy equation that includes wave-current interactions and dissipation representative of wave breaking.Then,the existing Finite-Volume Coastal Ocean Model(FVCOM) is modified to couple with the wave module.The couple procedure includes depth dependent wave radiation stress terms,Stokes drift,vertical transfer of wave-generated pressure transfer to the mean momentum equation,wave dissipation as a source term in the turbulence kinetic energy equation,and mean current advection and refraction of wave energy.Several applications are presented to evaluate the developed model.In particular the wind and wave-induced storm surge generated by Hurricane Katrina is investigated.The obtained results have been compared to the in situ measurements with respect to the wave heights and water level elevations revealing good accuracy of the model in reproduction of the investigated events.In a comparison to water level measurements at Dauphin Island,inclusion of the wave induced water level setup reduced the normalized root mean square error from 0.301 to 0.257 m and increased the correlation coefficient from 0.860 to 0.929.Several runs were carried out to analyze the effects of waves.The experiments show that among the processes that represent wave effects,radiation stress and wave-induced surface stress are more important than wave-induced bottom stress in affecting the water level.The Hurricane Katrina simulations showed the importance of the inclusion of the wave effects for the hindcast of the water levels during the storm surge.
