Cu2+ adsorption from simulated aqueous solution was investigated using a modified spent shiitake substrate (MSSS). The results showed that the MSSS has a high adsorption efficiency and removal performance. The Cu2+rem...Cu2+ adsorption from simulated aqueous solution was investigated using a modified spent shiitake substrate (MSSS). The results showed that the MSSS has a high adsorption efficiency and removal performance. The Cu2+removal rate of the MSSS reached above 95%. Compared with spent shiitake substrate (SSS), the specific surface area, electronegativity and surface functional groups of the MSSS were all improved, resulting in a high adsorption capacity. The Cu2+ adsorption of MSSS reached equilibrium after 0.75 h and was an exothermic reaction. The SEM and EDS analyses of the MSSS before and after Cu2+ adsorption showed that the pores on the surface of the MSSS were occupied after adsorption and the Cu content increased but the Na content decreased.展开更多
In this study, the ability of macroalgae Gracilaria sp. of removing eutrophication factors and toxic heavy metals A1, Cr, and Zn in a closed cultivation system is reported. The results show that the concentration of t...In this study, the ability of macroalgae Gracilaria sp. of removing eutrophication factors and toxic heavy metals A1, Cr, and Zn in a closed cultivation system is reported. The results show that the concentration of the three heavy metals decreased significantly during the experimental period in an algal biomass dependent manner. The biofiltration capacity of the alga for A1, Cr, and Zn is 10.1%-72.6%, 52.5%-83.4% and 36.5%,91.7%, respectively. Using more materials resulted in stronger heavy metal removal. Additionally, the concentration of chl-a, TN, TP and DIN of water samples fxom aquariums involving large, medium, and small algal biomass cultivation increased first and then decreased during the experiment. COD value of all three groups decreased with time and displayed algal biomass dependency： more algae resulting in a greater COD value than those of less biomass. Furthermore, changes in COD reflect an obvious organic particles deprivation process of algae. This is the first report on heavy metal removal effect by Gracilaria species. The results suggest that macroalgae can be used as a biofilter for the treatment of nutrient-enriched or heavy-metal polluted water, to which an appropriate time range should be carefully determined.展开更多
Thousand Seed Weight （TSW） is one of the major yield components of rapeseed （Brassica napus L.）. Here reports an extra-large seed germplasm GM01 which was obtained through isolated microspore culture. Three-way cr...Thousand Seed Weight （TSW） is one of the major yield components of rapeseed （Brassica napus L.）. Here reports an extra-large seed germplasm GM01 which was obtained through isolated microspore culture. Three-way cross was made： H8--a Yunnan spring early-maturing rapeseed variety, ＂Legeney＂--a Canadian canola variety and ＂020010＂--a semi-winter late-maturing rapeseed variety. One hundred and forty eight doubled hyploid lines were obtained from the F~ plants of three-way cross through isolated mierospore culture. Among them, the TSW of GM01 amounted to 8.68 g and the TSW of 53 lines were above 5.0 g. The TSWs of GM01 were relatively stable among the multi-location field trials from 2007 to 2014 with variation being only 10%-15% among the locations and years. Compared with H8, GM01 had larger flowers, stigmas, siliques and seed diameters, but less branches, siliques per plant and seeds per silique.展开更多
In vivo fluorescence methods are efficient tools for studying the distribution of phytoplankton in nature.Different algae species usually have different pigments with different ratios,which results in different fluore...In vivo fluorescence methods are efficient tools for studying the distribution of phytoplankton in nature.Different algae species usually have different pigments with different ratios,which results in different fluorescence emission spectra.Based on multiple excitation wavelength fluorescence emission spectra,a discrimination technique is established in this study.The discrimination method,established by multivariate linear regression and weighted least-squares,was used to differentiate the samples cultured in the laboratory and collected from Jiaozhou Bay near Qingdao at the division level.The correctly discriminated samples were ≥ 86% for single algae samples,≥ 88% for simulatively mixed ones,≥ 91% for physically mixed ones and 100% for samples collected from Jiaozhou Bay.The result in this research is more definite for the physically mixed samples in the laboratory.The method described here can be employed to monitor the phytoplankton population in the marine environment.展开更多
The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 1...The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 12 generations, respectively. Each generation was cultured in a media which is made from 4.43 g/L Murashige and Skoog （MS）, 30 g/L sucrose and 2 g/L gelrite, respectively, and grown under 16 h light and 8 h dark photoperiod for 30 d. For each generation, 45 plantlets were acclimatized for two months in a glasshouse. Data on in vitro growth performance and 11 morphological characteristics during acclimatization were recorded. Early root and shoot formation was observed after the 27th and 21st sub-cultured generations of Monate and Mokone, respectively. During acclimatization, plantlets from the same variety showed differences in morphological traits such as leaf colour, abaxial leaf pigmentation, vine pigmentation, petiole pigmentation, leaf wrinkling and flowering. However, the rate of these morphological differences is random and irrespective to increase in sub-culturing. Therefore, to understand the genetic base of these morphological variability, two plantlets from each variety were subjected to genetic analysis by using five simple sequence repeat （SSR） primers （IB-242, IB-318, IB-255F, 1B-248 and IB-255）. Although SSR loci IB-255F and IB-318 could distinguish between the three varieties, there were no allelic polymorphisms detected in plantlets from the same varieties. Therefore, long-term sub-culturing do not leads to quality degeneration in the three sweet potato varieties.展开更多
基金Project(51204011)supported by the National Natural Science Foundation of ChinaProject(20121000803)supported by the Foundation of the Advisor of Beijing Excellent Doctoral Dissertation of ChinaProject(2013T60063)supported by the China Postdoctoral Science Foundation
文摘Cu2+ adsorption from simulated aqueous solution was investigated using a modified spent shiitake substrate (MSSS). The results showed that the MSSS has a high adsorption efficiency and removal performance. The Cu2+removal rate of the MSSS reached above 95%. Compared with spent shiitake substrate (SSS), the specific surface area, electronegativity and surface functional groups of the MSSS were all improved, resulting in a high adsorption capacity. The Cu2+ adsorption of MSSS reached equilibrium after 0.75 h and was an exothermic reaction. The SEM and EDS analyses of the MSSS before and after Cu2+ adsorption showed that the pores on the surface of the MSSS were occupied after adsorption and the Cu content increased but the Na content decreased.
文摘In this study, the ability of macroalgae Gracilaria sp. of removing eutrophication factors and toxic heavy metals A1, Cr, and Zn in a closed cultivation system is reported. The results show that the concentration of the three heavy metals decreased significantly during the experimental period in an algal biomass dependent manner. The biofiltration capacity of the alga for A1, Cr, and Zn is 10.1%-72.6%, 52.5%-83.4% and 36.5%,91.7%, respectively. Using more materials resulted in stronger heavy metal removal. Additionally, the concentration of chl-a, TN, TP and DIN of water samples fxom aquariums involving large, medium, and small algal biomass cultivation increased first and then decreased during the experiment. COD value of all three groups decreased with time and displayed algal biomass dependency： more algae resulting in a greater COD value than those of less biomass. Furthermore, changes in COD reflect an obvious organic particles deprivation process of algae. This is the first report on heavy metal removal effect by Gracilaria species. The results suggest that macroalgae can be used as a biofilter for the treatment of nutrient-enriched or heavy-metal polluted water, to which an appropriate time range should be carefully determined.
文摘Thousand Seed Weight （TSW） is one of the major yield components of rapeseed （Brassica napus L.）. Here reports an extra-large seed germplasm GM01 which was obtained through isolated microspore culture. Three-way cross was made： H8--a Yunnan spring early-maturing rapeseed variety, ＂Legeney＂--a Canadian canola variety and ＂020010＂--a semi-winter late-maturing rapeseed variety. One hundred and forty eight doubled hyploid lines were obtained from the F~ plants of three-way cross through isolated mierospore culture. Among them, the TSW of GM01 amounted to 8.68 g and the TSW of 53 lines were above 5.0 g. The TSWs of GM01 were relatively stable among the multi-location field trials from 2007 to 2014 with variation being only 10%-15% among the locations and years. Compared with H8, GM01 had larger flowers, stigmas, siliques and seed diameters, but less branches, siliques per plant and seeds per silique.
基金supported by the National Natural Science Foundation of China (No.40706036)the National High-Tech Research and Development Program of China (863 Program) (No.2006AA09Z178)
文摘In vivo fluorescence methods are efficient tools for studying the distribution of phytoplankton in nature.Different algae species usually have different pigments with different ratios,which results in different fluorescence emission spectra.Based on multiple excitation wavelength fluorescence emission spectra,a discrimination technique is established in this study.The discrimination method,established by multivariate linear regression and weighted least-squares,was used to differentiate the samples cultured in the laboratory and collected from Jiaozhou Bay near Qingdao at the division level.The correctly discriminated samples were ≥ 86% for single algae samples,≥ 88% for simulatively mixed ones,≥ 91% for physically mixed ones and 100% for samples collected from Jiaozhou Bay.The result in this research is more definite for the physically mixed samples in the laboratory.The method described here can be employed to monitor the phytoplankton population in the marine environment.
文摘The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 12 generations, respectively. Each generation was cultured in a media which is made from 4.43 g/L Murashige and Skoog （MS）, 30 g/L sucrose and 2 g/L gelrite, respectively, and grown under 16 h light and 8 h dark photoperiod for 30 d. For each generation, 45 plantlets were acclimatized for two months in a glasshouse. Data on in vitro growth performance and 11 morphological characteristics during acclimatization were recorded. Early root and shoot formation was observed after the 27th and 21st sub-cultured generations of Monate and Mokone, respectively. During acclimatization, plantlets from the same variety showed differences in morphological traits such as leaf colour, abaxial leaf pigmentation, vine pigmentation, petiole pigmentation, leaf wrinkling and flowering. However, the rate of these morphological differences is random and irrespective to increase in sub-culturing. Therefore, to understand the genetic base of these morphological variability, two plantlets from each variety were subjected to genetic analysis by using five simple sequence repeat （SSR） primers （IB-242, IB-318, IB-255F, 1B-248 and IB-255）. Although SSR loci IB-255F and IB-318 could distinguish between the three varieties, there were no allelic polymorphisms detected in plantlets from the same varieties. Therefore, long-term sub-culturing do not leads to quality degeneration in the three sweet potato varieties.
