A complete hydatidiform mole coexisting with a fetus following in vitro fertilization and embryo transfer (IVF-ET) is a rare event. The diagnosis is often not easy because of the morphological similarity to a partial ...A complete hydatidiform mole coexisting with a fetus following in vitro fertilization and embryo transfer (IVF-ET) is a rare event. The diagnosis is often not easy because of the morphological similarity to a partial mole, but important to the treatment. We present a recent case in which STR polymorphism analysis clearly revealed a different genetic origin for the fetal and molar parts. STR polymorphisms on 15 variable number tandem repeat loci and a gender-determination locus, which were detected by polymerase chain reaction, indicating that the cord/placenta and molar tissue were parental and androgenous, respectively. During follow-up, the patient developed persistent gestational trophoblastic tumor (GTT) which was successfully treated with chemotherapy. In this case, STR polymorphism analysis exactly diagnosed a twin pregnancy consisting of a complete hydatidiform mole and a fetus.展开更多
沙门氏菌是一类危害人和动物健康的重要致病菌,是引起食物中毒的最常见病原菌之一。本文对目前常用的3种分子分型方法脉冲场凝胶电泳(Pulsed-field gel electrophoresis,PFGE),多位点序列分析(Multi-locus sequence analysis,MLST)及多...沙门氏菌是一类危害人和动物健康的重要致病菌,是引起食物中毒的最常见病原菌之一。本文对目前常用的3种分子分型方法脉冲场凝胶电泳(Pulsed-field gel electrophoresis,PFGE),多位点序列分析(Multi-locus sequence analysis,MLST)及多位点可变数串联重复分析(Multiple-locus variable number tandem repeats analysis,MLVA)在沙门氏菌分子分型的应用做了阐述和比较,为沙门氏菌分型研究提供一定的参考。展开更多
炭疽芽孢杆菌是分子多样性较低的革兰阳性菌,具有高度基因单态性。现有的基因分型方法中仅有少数几种适用于该菌株间的基因分型研究,如多位点可变数串联重复序列分析(multiple locus variable number tandem repeat analysis,MLVA)和单...炭疽芽孢杆菌是分子多样性较低的革兰阳性菌,具有高度基因单态性。现有的基因分型方法中仅有少数几种适用于该菌株间的基因分型研究,如多位点可变数串联重复序列分析(multiple locus variable number tandem repeat analysis,MLVA)和单核苷酸多态性(single nucleotide polymorphism,SNP)分析等。该文综述了MLVA法和SNP法在炭疽芽孢杆菌菌株间分型中的应用进展。展开更多
The gravity field models GUCAS_EGM and GUCAS_EGM_DL are established from GOCE data (GOCE Level 2 Products from Nov. 1 to Dec. 31, 2009) based on the method of the invariants of the gravity gradient tensor, where GUCAS...The gravity field models GUCAS_EGM and GUCAS_EGM_DL are established from GOCE data (GOCE Level 2 Products from Nov. 1 to Dec. 31, 2009) based on the method of the invariants of the gravity gradient tensor, where GUCAS_EGM is derived after GOCE gravity gradient data are filtered with FIR, and GUCAS_EGM_DL is computed with an additional Durbin-Levison arithmetic apart from FIR. Since this method, different from current programs dealing with GOCE data, is introduced for the first time, some new problems are required to be discussed in advance; for example, how to filter GOCE gravity gradient data, how to compute the invariants of the gradient tensor, and how to deal with the pole gap and so on. In addition, by comparing our models with ones recommended by ESA, it can be seen that the variations of GUCAS_EGM and the models recommended by ESA to EGM08 are almost equivalent, and the variation of GUCAS_EGM_DL to EGM08 is obviously less than ones of the recommended models.展开更多
基金Supported by Production and Rresearch Projects of Guangdong Province (2007B090400140)
文摘A complete hydatidiform mole coexisting with a fetus following in vitro fertilization and embryo transfer (IVF-ET) is a rare event. The diagnosis is often not easy because of the morphological similarity to a partial mole, but important to the treatment. We present a recent case in which STR polymorphism analysis clearly revealed a different genetic origin for the fetal and molar parts. STR polymorphisms on 15 variable number tandem repeat loci and a gender-determination locus, which were detected by polymerase chain reaction, indicating that the cord/placenta and molar tissue were parental and androgenous, respectively. During follow-up, the patient developed persistent gestational trophoblastic tumor (GTT) which was successfully treated with chemotherapy. In this case, STR polymorphism analysis exactly diagnosed a twin pregnancy consisting of a complete hydatidiform mole and a fetus.
文摘沙门氏菌是一类危害人和动物健康的重要致病菌,是引起食物中毒的最常见病原菌之一。本文对目前常用的3种分子分型方法脉冲场凝胶电泳(Pulsed-field gel electrophoresis,PFGE),多位点序列分析(Multi-locus sequence analysis,MLST)及多位点可变数串联重复分析(Multiple-locus variable number tandem repeats analysis,MLVA)在沙门氏菌分子分型的应用做了阐述和比较,为沙门氏菌分型研究提供一定的参考。
文摘炭疽芽孢杆菌是分子多样性较低的革兰阳性菌,具有高度基因单态性。现有的基因分型方法中仅有少数几种适用于该菌株间的基因分型研究,如多位点可变数串联重复序列分析(multiple locus variable number tandem repeat analysis,MLVA)和单核苷酸多态性(single nucleotide polymorphism,SNP)分析等。该文综述了MLVA法和SNP法在炭疽芽孢杆菌菌株间分型中的应用进展。
基金supported by National Natural Science Foundation of China (Grant No.41074015)Program of Chinese Academy of Sciences (Grant No.XMXX280730)
文摘The gravity field models GUCAS_EGM and GUCAS_EGM_DL are established from GOCE data (GOCE Level 2 Products from Nov. 1 to Dec. 31, 2009) based on the method of the invariants of the gravity gradient tensor, where GUCAS_EGM is derived after GOCE gravity gradient data are filtered with FIR, and GUCAS_EGM_DL is computed with an additional Durbin-Levison arithmetic apart from FIR. Since this method, different from current programs dealing with GOCE data, is introduced for the first time, some new problems are required to be discussed in advance; for example, how to filter GOCE gravity gradient data, how to compute the invariants of the gradient tensor, and how to deal with the pole gap and so on. In addition, by comparing our models with ones recommended by ESA, it can be seen that the variations of GUCAS_EGM and the models recommended by ESA to EGM08 are almost equivalent, and the variation of GUCAS_EGM_DL to EGM08 is obviously less than ones of the recommended models.
