For many years, computer systems have emerged; they now occupy an important place in our daily lives. The growing needs and ever increasing use of computer systems have made application development more and more compl...For many years, computer systems have emerged; they now occupy an important place in our daily lives. The growing needs and ever increasing use of computer systems have made application development more and more complicated, The complexity of these applications poses problems such as reuse, installation, administration and evolution of applications. The development of applications is related to the evolution of paradigms and approaches to developing them. This paper presents different approaches and paradigms of development starting with the procedural approach, coming up for service, through the component and object-oriented approaches. Also, for each of the approaches we determine the advantages and limitations.展开更多
The Cyclophragma undans nucleopolyhedrovirus （CyunNPV）, a potential pest control agent, was isolated from Cyclophragma undans （Lepidoptera： Lasiocampidae）, an important forest pest. In the present study, we perfo...The Cyclophragma undans nucleopolyhedrovirus （CyunNPV）, a potential pest control agent, was isolated from Cyclophragma undans （Lepidoptera： Lasiocampidae）, an important forest pest. In the present study, we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group I alphabaculoviruses. Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G ＋ C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group I Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to CyunNPV. In addition, the genome contains 13 homologous repeated sequences （hrs）. Phylogenetic analysis groups CyunNPV under a distinct branch within clade ＂a＂ of Group I in the genus Alphabaculovirus. Unlike other members of Group I, CyunNPV harbors only nine of the 11 genes previously determined to be specific to Group I viruses. Furthermore, the CyunNPV lacks the tyrosine phosphatase gene and the ac30 gene. The CyunNPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pretransmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings imply that CyunNPV is a distinct species under Group I Alphabaculovirus.展开更多
文摘For many years, computer systems have emerged; they now occupy an important place in our daily lives. The growing needs and ever increasing use of computer systems have made application development more and more complicated, The complexity of these applications poses problems such as reuse, installation, administration and evolution of applications. The development of applications is related to the evolution of paradigms and approaches to developing them. This paper presents different approaches and paradigms of development starting with the procedural approach, coming up for service, through the component and object-oriented approaches. Also, for each of the approaches we determine the advantages and limitations.
基金supported by the National Key R&D Program of China(Grant No.2017YFD0200400)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB11030400)
文摘The Cyclophragma undans nucleopolyhedrovirus （CyunNPV）, a potential pest control agent, was isolated from Cyclophragma undans （Lepidoptera： Lasiocampidae）, an important forest pest. In the present study, we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group I alphabaculoviruses. Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G ＋ C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group I Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to CyunNPV. In addition, the genome contains 13 homologous repeated sequences （hrs）. Phylogenetic analysis groups CyunNPV under a distinct branch within clade ＂a＂ of Group I in the genus Alphabaculovirus. Unlike other members of Group I, CyunNPV harbors only nine of the 11 genes previously determined to be specific to Group I viruses. Furthermore, the CyunNPV lacks the tyrosine phosphatase gene and the ac30 gene. The CyunNPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pretransmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings imply that CyunNPV is a distinct species under Group I Alphabaculovirus.
