In this paper, we produce porous silicon （PSi） by electrochemical etching, and it is the first time to evaluate the performance of label-free porous silicon biosensor for detection of variable domain of heavy chain ...In this paper, we produce porous silicon （PSi） by electrochemical etching, and it is the first time to evaluate the performance of label-free porous silicon biosensor for detection of variable domain of heavy chain of heavy-chain antibody （VHH）. The binding of hen egg white lysozyme （HEWL） and VHH causes a red shift in the reflection spectrum of the biosensor. The red shift is proportional to the VHH concenlration in the range from 14 gg.ml-I to 30 pg.ml~ with a detection limit of 0.648 ng.ml~. The research is useful for the development of label-free biosensor applied in the rapid and sensitive determination of small molecules.展开更多
基金supported by the National Natural Science Foundation of China (No.60968002)
文摘In this paper, we produce porous silicon （PSi） by electrochemical etching, and it is the first time to evaluate the performance of label-free porous silicon biosensor for detection of variable domain of heavy chain of heavy-chain antibody （VHH）. The binding of hen egg white lysozyme （HEWL） and VHH causes a red shift in the reflection spectrum of the biosensor. The red shift is proportional to the VHH concenlration in the range from 14 gg.ml-I to 30 pg.ml~ with a detection limit of 0.648 ng.ml~. The research is useful for the development of label-free biosensor applied in the rapid and sensitive determination of small molecules.
