High rate of relapse to drug using behavior after long period of abstinence cha racterizes the behavior of experienced users of heroin and other drugs of abuse, and the relapse remains the primary problem for treatmen...High rate of relapse to drug using behavior after long period of abstinence cha racterizes the behavior of experienced users of heroin and other drugs of abuse, and the relapse remains the primary problem for treatment. In the present study we built a putative animal model that mimic human relapse i.e., the reinstatement o f morph ine induced conditioned place preference (CPP) in rats. In this study, we found electroacupuncture (EA) with low frequency (2 and 2/100 Hz) could inhibit drug priming or footshock induced CPP reinstatement in rats whe n it was given 18 hours before reinstatement, and these effects were found to be naloxone reversible, suggesting a mechanism involving the activation of opioid receptors by endogenous opioid ligands; while EA with high frequency (100 Hz) h ad no effect. Pr evious studies in our lab have amply shown that low frequency (2 Hz) stimulation could increase the release of enkephalin which acts on μ and δ opioid recepto rs up the spinal level, while high frequency (100 Hz) stimulation could increase the release of dynorphin which interacts with κ opioid receptor at spinal leve l. So we concluded the effect of EA with low frequency on relapse involving a me chanism of the activation of opioid receptors by endogenous opioid ligands above the spinal level. And we suggest that EA may be used as a putative measure for the prevention of relapse to drug use in humans.展开更多
The ascidian Ciona intestinalis is a model organism of developmental and evolutionary biology and may provide orucial clues concerning two fundamental matters, namely, how chordates originated from the putative deuter...The ascidian Ciona intestinalis is a model organism of developmental and evolutionary biology and may provide orucial clues concerning two fundamental matters, namely, how chordates originated from the putative deuterostome ancestor and how advanced chordates originated from the simplest chordates. In this paper, a whole-life-span culture of C. intestinalis was conducted. Fed with the diet combination of dry Spirulina, egg yolk, Dicrateria sp., edible yeast and weaning diet for shrimp, C. intestinalis grew up to average 59 mm and matured after 60 d cultivation. This culture process could be repeated using the artificially cultured mature ascidians as material. When the fertilized eggs were maintained under 10, 15, 20, 25 ℃, they hatched within 30 h, 22 h, 16 h and 12 h 50 min respectively experiencing cleavage, blastulation, gastrulation, neurulation, tailbud stage and tadpole stage, The tadpole larvae were characterized as typical but simplified chordates because of their dorsal nerve cord, notochord and primordial brain. After 8-24 h freely swimming, the tadpole larvae settled on the substrates and metamorphosized within 1-2 d into filter feeding sessile juvenile ascidians. In addition, unfertilized eggs were successfully dechorionated in filtered seawater containing 1% Tripsin, 0.25% EDTA at pH of 10.5 within 40 min. After fertilization, the dechorionated eggs developed well and hatched at normal hatching rate. In conclusion, this paper presented feasible methodology for rearing the tadpole larvae of C. intestinalis into sexual maturity under controlled conditions and detailed observations on the embryogenesis of the laboratory cultured ascidians, which will facilitate developmental and genetic research using this model system.展开更多
Seismic data typically contain random missing traces because of obstacles and economic restrictions,influencing subsequent processing and interpretation.Seismic data recovery can be expressed as a low-rank matrix appr...Seismic data typically contain random missing traces because of obstacles and economic restrictions,influencing subsequent processing and interpretation.Seismic data recovery can be expressed as a low-rank matrix approximation problem by assuming a low-rank structure for the complete seismic data in the frequency–space(f–x)domain.The nuclear norm minimization(NNM)(sum of singular values)approach treats singular values equally,yielding a solution deviating from the optimal.Further,the log-sum majorization–minimization(LSMM)approach uses the nonconvex log-sum function as a rank substitution for seismic data interpolation,which is highly accurate but time-consuming.Therefore,this study proposes an efficient nonconvex reconstruction model based on the nonconvex Geman function(the nonconvex Geman low-rank(NCGL)model),involving a tighter approximation of the original rank function.Without introducing additional parameters,the nonconvex problem is solved using the Karush–Kuhn–Tucker condition theory.Experiments using synthetic and field data demonstrate that the proposed NCGL approach achieves a higher signal-to-noise ratio than the singular value thresholding method based on NNM and the projection onto convex sets method based on the data-driven threshold model.The proposed approach achieves higher reconstruction efficiency than the singular value thresholding and LSMM methods.展开更多
A new method is proposed to inverse normalization data of hidden variables in a dynamical system by embedding a time series in multidimensional spaces and applying a normalization analysis to the conditional probabili...A new method is proposed to inverse normalization data of hidden variables in a dynamical system by embedding a time series in multidimensional spaces and applying a normalization analysis to the conditional probability density of points in the reconstructed phase spaces. The method is robust in the application to Lorenz system and 4-dimensional R?ssler system by testing quantitatively and qualitatively the correlation coefficient between inverse data and original data in time domain and in frequency domain, respectively. By applying the method to analyzing the South China Sea data, the normalization data of wind speed is extracted from the sea surface temperatu-re time series.展开更多
文摘High rate of relapse to drug using behavior after long period of abstinence cha racterizes the behavior of experienced users of heroin and other drugs of abuse, and the relapse remains the primary problem for treatment. In the present study we built a putative animal model that mimic human relapse i.e., the reinstatement o f morph ine induced conditioned place preference (CPP) in rats. In this study, we found electroacupuncture (EA) with low frequency (2 and 2/100 Hz) could inhibit drug priming or footshock induced CPP reinstatement in rats whe n it was given 18 hours before reinstatement, and these effects were found to be naloxone reversible, suggesting a mechanism involving the activation of opioid receptors by endogenous opioid ligands; while EA with high frequency (100 Hz) h ad no effect. Pr evious studies in our lab have amply shown that low frequency (2 Hz) stimulation could increase the release of enkephalin which acts on μ and δ opioid recepto rs up the spinal level, while high frequency (100 Hz) stimulation could increase the release of dynorphin which interacts with κ opioid receptor at spinal leve l. So we concluded the effect of EA with low frequency on relapse involving a me chanism of the activation of opioid receptors by endogenous opioid ligands above the spinal level. And we suggest that EA may be used as a putative measure for the prevention of relapse to drug use in humans.
基金Project supported by the Hi-Tech Research and Development Pro-gram (863) of China (No. 2003AA603440) and the Knowledge In-novation Program from the Chinese Academy of Sciences (No.KZCX2-211)
文摘The ascidian Ciona intestinalis is a model organism of developmental and evolutionary biology and may provide orucial clues concerning two fundamental matters, namely, how chordates originated from the putative deuterostome ancestor and how advanced chordates originated from the simplest chordates. In this paper, a whole-life-span culture of C. intestinalis was conducted. Fed with the diet combination of dry Spirulina, egg yolk, Dicrateria sp., edible yeast and weaning diet for shrimp, C. intestinalis grew up to average 59 mm and matured after 60 d cultivation. This culture process could be repeated using the artificially cultured mature ascidians as material. When the fertilized eggs were maintained under 10, 15, 20, 25 ℃, they hatched within 30 h, 22 h, 16 h and 12 h 50 min respectively experiencing cleavage, blastulation, gastrulation, neurulation, tailbud stage and tadpole stage, The tadpole larvae were characterized as typical but simplified chordates because of their dorsal nerve cord, notochord and primordial brain. After 8-24 h freely swimming, the tadpole larvae settled on the substrates and metamorphosized within 1-2 d into filter feeding sessile juvenile ascidians. In addition, unfertilized eggs were successfully dechorionated in filtered seawater containing 1% Tripsin, 0.25% EDTA at pH of 10.5 within 40 min. After fertilization, the dechorionated eggs developed well and hatched at normal hatching rate. In conclusion, this paper presented feasible methodology for rearing the tadpole larvae of C. intestinalis into sexual maturity under controlled conditions and detailed observations on the embryogenesis of the laboratory cultured ascidians, which will facilitate developmental and genetic research using this model system.
基金financially supported by the National Key R&D Program of China(No.2018YFC1503705)the Science and Technology Research Project of Hubei Provincial Department of Education(No.B2017597)+1 种基金the Hubei Subsurface Multiscale Imaging Key Laboratory(China University of Geosciences)(No.SMIL-2018-06)the Fundamental Research Funds for the Central Universities(No.CCNU19TS020).
文摘Seismic data typically contain random missing traces because of obstacles and economic restrictions,influencing subsequent processing and interpretation.Seismic data recovery can be expressed as a low-rank matrix approximation problem by assuming a low-rank structure for the complete seismic data in the frequency–space(f–x)domain.The nuclear norm minimization(NNM)(sum of singular values)approach treats singular values equally,yielding a solution deviating from the optimal.Further,the log-sum majorization–minimization(LSMM)approach uses the nonconvex log-sum function as a rank substitution for seismic data interpolation,which is highly accurate but time-consuming.Therefore,this study proposes an efficient nonconvex reconstruction model based on the nonconvex Geman function(the nonconvex Geman low-rank(NCGL)model),involving a tighter approximation of the original rank function.Without introducing additional parameters,the nonconvex problem is solved using the Karush–Kuhn–Tucker condition theory.Experiments using synthetic and field data demonstrate that the proposed NCGL approach achieves a higher signal-to-noise ratio than the singular value thresholding method based on NNM and the projection onto convex sets method based on the data-driven threshold model.The proposed approach achieves higher reconstruction efficiency than the singular value thresholding and LSMM methods.
基金the National Natural Science Foundation of China (Grant No.49476254).
文摘A new method is proposed to inverse normalization data of hidden variables in a dynamical system by embedding a time series in multidimensional spaces and applying a normalization analysis to the conditional probability density of points in the reconstructed phase spaces. The method is robust in the application to Lorenz system and 4-dimensional R?ssler system by testing quantitatively and qualitatively the correlation coefficient between inverse data and original data in time domain and in frequency domain, respectively. By applying the method to analyzing the South China Sea data, the normalization data of wind speed is extracted from the sea surface temperatu-re time series.
