In this study, comparative heat flux measurements for a sharp cone model were conducted by utilizing a high enthalpy shock tunnel JF-10 and a large-scale shock tunnel JF-12, responsible for providing nonequilibrium an...In this study, comparative heat flux measurements for a sharp cone model were conducted by utilizing a high enthalpy shock tunnel JF-10 and a large-scale shock tunnel JF-12, responsible for providing nonequilibrium and perfect gas flows, respectively. Experiments were performed at the Key Laboratory of High Temperature Gas Dynamics(LHD), Institute of Mechanics, Chinese Academy of Sciences. Corresponding numerical simulations were also conducted in effort to better understand the phenomena accompanying in these experiments. By assessing the consistency and accuracy of all the data gathered during this study, a detailed comparison of sharp cone heat transfer under a totally different kind of freestream conditions was build and analyzed. One specific parameter, defined as the product of the Stanton number and the square root of the Reynold number, was found to be more characteristic for the aerodynamic heating phenomena encountered in hypersonic flight. Adequate use of said parameter practically eliminates the variability caused by the deferent flow conditions, regardless of whether the flow is in dissociation or the boundary condition is catalytic. Essentially, the parameter identified in this study reduces the amount of ground experimental data necessary and eases data extrapolation to flight.展开更多
To promote spinosad biosynthesis by improving the limited oxygen supply during high-density fermentation of Saccharopolyspora spinosa, the open reading frame of the Vitreoscilla hemoglobin gene was placed under the co...To promote spinosad biosynthesis by improving the limited oxygen supply during high-density fermentation of Saccharopolyspora spinosa, the open reading frame of the Vitreoscilla hemoglobin gene was placed under the control of the promoter for the erythromycin resistance gene by splicing using overlapping extension PCR. This was cloned into the integrating vector pSET152, yielding the Vitreoscilla hemoglobin gene expression plasmid pSET152EVHB. This was then introduced into S. spinosa SP06081 by conjugal transfer, and integrated into the chromosome by site-specific recombination at the integration site ФC31 on pSET152EVHB. The resultant conjugant, S. spinosa S078-1101, was genetically stable. The integration was further confirmed by PCR and Southern blotting analysis. A carbon monoxide differential spectrum assay showed that active Vitreoscilla hemoglobin was successfully expressed in S. spinosa S078-1101. Fermentation results revealed that expression of the Vitreoscilla hemoglobin gene significantly promoted spinosad biosynthesis under normal oxygen and moderately oxygen-limiting conditions （P〈0.01）. These findings demonstrate that integrating expression of the Vitreoscilla hemoglobin gene improves oxygen uptake and is an effective means for the genetic improvement of S. spinosa fermentation. Saccharopolyspora spinosa, spinosad, Vitreoscilla hemoglobin, integrating vector, homologous recombination展开更多
基金the National Natural Science Foundation of China(Grant Nos.1140227511472280 and 11532014)
文摘In this study, comparative heat flux measurements for a sharp cone model were conducted by utilizing a high enthalpy shock tunnel JF-10 and a large-scale shock tunnel JF-12, responsible for providing nonequilibrium and perfect gas flows, respectively. Experiments were performed at the Key Laboratory of High Temperature Gas Dynamics(LHD), Institute of Mechanics, Chinese Academy of Sciences. Corresponding numerical simulations were also conducted in effort to better understand the phenomena accompanying in these experiments. By assessing the consistency and accuracy of all the data gathered during this study, a detailed comparison of sharp cone heat transfer under a totally different kind of freestream conditions was build and analyzed. One specific parameter, defined as the product of the Stanton number and the square root of the Reynold number, was found to be more characteristic for the aerodynamic heating phenomena encountered in hypersonic flight. Adequate use of said parameter practically eliminates the variability caused by the deferent flow conditions, regardless of whether the flow is in dissociation or the boundary condition is catalytic. Essentially, the parameter identified in this study reduces the amount of ground experimental data necessary and eases data extrapolation to flight.
基金supported by the National Basic Research Program of China (Grant Nos. 2012CB722301 and 2011CB111605)the National High Technology Research and Development Project of China (Grant No. 2011AA10A203)the National Natural Science Foundation of China (Grant No. 31070006)
文摘To promote spinosad biosynthesis by improving the limited oxygen supply during high-density fermentation of Saccharopolyspora spinosa, the open reading frame of the Vitreoscilla hemoglobin gene was placed under the control of the promoter for the erythromycin resistance gene by splicing using overlapping extension PCR. This was cloned into the integrating vector pSET152, yielding the Vitreoscilla hemoglobin gene expression plasmid pSET152EVHB. This was then introduced into S. spinosa SP06081 by conjugal transfer, and integrated into the chromosome by site-specific recombination at the integration site ФC31 on pSET152EVHB. The resultant conjugant, S. spinosa S078-1101, was genetically stable. The integration was further confirmed by PCR and Southern blotting analysis. A carbon monoxide differential spectrum assay showed that active Vitreoscilla hemoglobin was successfully expressed in S. spinosa S078-1101. Fermentation results revealed that expression of the Vitreoscilla hemoglobin gene significantly promoted spinosad biosynthesis under normal oxygen and moderately oxygen-limiting conditions （P〈0.01）. These findings demonstrate that integrating expression of the Vitreoscilla hemoglobin gene improves oxygen uptake and is an effective means for the genetic improvement of S. spinosa fermentation. Saccharopolyspora spinosa, spinosad, Vitreoscilla hemoglobin, integrating vector, homologous recombination
