目的观察重组人干扰素α-2b眼液对小梁切除术后手术效果的影响。方法将我院40例行小梁切除术的青光眼患者随机分为A、B两组,两组均常规眼球按摩,百力特眼液应用1月。A组20例术后3d开始点用重组人干扰素α-2b眼液,4次/d,持续8周;B组20例...目的观察重组人干扰素α-2b眼液对小梁切除术后手术效果的影响。方法将我院40例行小梁切除术的青光眼患者随机分为A、B两组,两组均常规眼球按摩,百力特眼液应用1月。A组20例术后3d开始点用重组人干扰素α-2b眼液,4次/d,持续8周;B组20例不使用干扰素眼液。随访3个月,观察两组术后1周、1月、2月、3月的眼压,功能性滤过泡形成情况以及眼部不适症状。结果术后1周,平均眼压:A组11 mm Hg,B组12 mm Hg;两组功能性滤过泡均形成良好;结膜上皮及角膜上皮无明显荧光素染色,患者均有轻度的结膜充血及异物感。术后1月,A组眼压均<21 mm Hg,平均眼压13 mm Hg;B组2例眼压>21 mm Hg,分别为26 mm Hg、28 mm Hg,平均眼压为15 mm Hg。A组:Ⅰ型滤过泡(微小囊泡型)15例(75%),Ⅱ型滤过泡(弥漫扁平型)5例;B组:Ⅰ型滤过泡10例(50%),Ⅱ型滤过泡8例,滤过泡轻度包裹2例,按摩眼球等处理后恢复功能,1例有轻度异物感。术后2月,两组眼压均<21 mm Hg,平均眼压:A组14 mm Hg,B组16 mm Hg;A组Ⅰ型滤过泡13例(65%),Ⅱ型滤过泡7例,B组Ⅰ型滤过泡8例(40%),Ⅱ型滤过泡12例,两组均有轻度结膜充血,异物感。术后3月,两组眼压均<21 mm Hg,平均眼压:A组15mm Hg,B组18mm Hg;A组Ⅰ型滤过泡12例(60%),Ⅱ型滤过泡8例,B组Ⅰ型滤过泡8例(40%),Ⅱ型滤过泡12例,两组均无明显结膜充血,异物感。结论小梁切除术后10~14d是成纤维细胞趋化增生的关键时刻。术后3d开始点用重组人干扰素α-2b眼液,持续8周,对成纤维细胞有明显的抑制作用,能获得更低的眼压,对术后短期内形成Ⅰ型功能滤过泡有促进作用,且无明显不良反应,是小梁切除术后提高手术成功率的一个良好的辅助方法。展开更多
Since recombinant human endostatin (rh-endostatin;Endostar) has been listed 5 years,clinicians have combined it with chemotherapy for the treatment of lung cancers and other malignant tumors,and proved its effect and ...Since recombinant human endostatin (rh-endostatin;Endostar) has been listed 5 years,clinicians have combined it with chemotherapy for the treatment of lung cancers and other malignant tumors,and proved its effect and safety.A number of scholars have explored the application of Endostar alone or in combination with chemotherapy for treatment of malignant serous effusion,finding its high efficiency and low toxicity;and that hydrops controlling is stronger,and that it can significantly improve patients' quality of life.It is worthy of conducting prospective,randomized and multi-center clinical studies and basic researches to clarify the mechanism.展开更多
Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover ...Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.展开更多
Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four importan...Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was （377.9±1.7） mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gil2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.展开更多
Plasmid DNA, an effective vaccine vector, can induce both cellular and humoral immune responses. However, plasmid DNA raises issues concerning potential genomic integration after injection. This issue should be consid...Plasmid DNA, an effective vaccine vector, can induce both cellular and humoral immune responses. However, plasmid DNA raises issues concerning potential genomic integration after injection. This issue should be considered in preclinical studies. Tiantan vaccinia virus (TV) has been most widely utilized in eradicating smallpox in China. This virus has also been considered as a successful vaccine vector against a few infectious diseases. Potent T cell responses through T-cell receptor (TCR) could be induced by three injections of the DNA prime vaccine followed by a single injection of recombinant vaccinia vaccine. To develop a safer immunization strategy, a single DNA prime followed by a single recombinant Tiantan vaccinia (rTV) AIDS vaccine was used to immunize mice. Our data demonstrated that one DNA prime/rTV boost regimen induced mature TCR activation with high functional avidity, preferential T cell Vβ receptor usage and high sensitivity to anti-CD3 antibody stimulation. No differences in T cell responses were observed among one, two or three DNA prime/rTV boost regimens. This study shows that one DNA prime/rTV boost regimen is sufficient to induce potent T cell responses against HIV.展开更多
文摘目的观察重组人干扰素α-2b眼液对小梁切除术后手术效果的影响。方法将我院40例行小梁切除术的青光眼患者随机分为A、B两组,两组均常规眼球按摩,百力特眼液应用1月。A组20例术后3d开始点用重组人干扰素α-2b眼液,4次/d,持续8周;B组20例不使用干扰素眼液。随访3个月,观察两组术后1周、1月、2月、3月的眼压,功能性滤过泡形成情况以及眼部不适症状。结果术后1周,平均眼压:A组11 mm Hg,B组12 mm Hg;两组功能性滤过泡均形成良好;结膜上皮及角膜上皮无明显荧光素染色,患者均有轻度的结膜充血及异物感。术后1月,A组眼压均<21 mm Hg,平均眼压13 mm Hg;B组2例眼压>21 mm Hg,分别为26 mm Hg、28 mm Hg,平均眼压为15 mm Hg。A组:Ⅰ型滤过泡(微小囊泡型)15例(75%),Ⅱ型滤过泡(弥漫扁平型)5例;B组:Ⅰ型滤过泡10例(50%),Ⅱ型滤过泡8例,滤过泡轻度包裹2例,按摩眼球等处理后恢复功能,1例有轻度异物感。术后2月,两组眼压均<21 mm Hg,平均眼压:A组14 mm Hg,B组16 mm Hg;A组Ⅰ型滤过泡13例(65%),Ⅱ型滤过泡7例,B组Ⅰ型滤过泡8例(40%),Ⅱ型滤过泡12例,两组均有轻度结膜充血,异物感。术后3月,两组眼压均<21 mm Hg,平均眼压:A组15mm Hg,B组18mm Hg;A组Ⅰ型滤过泡12例(60%),Ⅱ型滤过泡8例,B组Ⅰ型滤过泡8例(40%),Ⅱ型滤过泡12例,两组均无明显结膜充血,异物感。结论小梁切除术后10~14d是成纤维细胞趋化增生的关键时刻。术后3d开始点用重组人干扰素α-2b眼液,持续8周,对成纤维细胞有明显的抑制作用,能获得更低的眼压,对术后短期内形成Ⅰ型功能滤过泡有促进作用,且无明显不良反应,是小梁切除术后提高手术成功率的一个良好的辅助方法。
基金Supported by a grant of Key Medical Issue of Nanjing Military Region (No.2007-012007-06)
文摘Since recombinant human endostatin (rh-endostatin;Endostar) has been listed 5 years,clinicians have combined it with chemotherapy for the treatment of lung cancers and other malignant tumors,and proved its effect and safety.A number of scholars have explored the application of Endostar alone or in combination with chemotherapy for treatment of malignant serous effusion,finding its high efficiency and low toxicity;and that hydrops controlling is stronger,and that it can significantly improve patients' quality of life.It is worthy of conducting prospective,randomized and multi-center clinical studies and basic researches to clarify the mechanism.
基金Supported by the Agriculture Application Investigation and Improvement Item of New Countryside Construction and Promotion Project of the Bureau of Science and Technology in Xi’an (NC08005)
文摘Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.
基金Supported by the National Natural Science Foundation of China （21036005）.
文摘Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was （377.9±1.7） mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gil2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.
基金supported by the China Comprehensive Integrated Programs for Research on AIDS(CIPRA, U19AI51915)by the National Key Projects on Major Infectious Diseases (Grant No. 2008ZX10001-010,2012ZX10001-008)by the National Natural Science Foundation of China (31000413)
文摘Plasmid DNA, an effective vaccine vector, can induce both cellular and humoral immune responses. However, plasmid DNA raises issues concerning potential genomic integration after injection. This issue should be considered in preclinical studies. Tiantan vaccinia virus (TV) has been most widely utilized in eradicating smallpox in China. This virus has also been considered as a successful vaccine vector against a few infectious diseases. Potent T cell responses through T-cell receptor (TCR) could be induced by three injections of the DNA prime vaccine followed by a single injection of recombinant vaccinia vaccine. To develop a safer immunization strategy, a single DNA prime followed by a single recombinant Tiantan vaccinia (rTV) AIDS vaccine was used to immunize mice. Our data demonstrated that one DNA prime/rTV boost regimen induced mature TCR activation with high functional avidity, preferential T cell Vβ receptor usage and high sensitivity to anti-CD3 antibody stimulation. No differences in T cell responses were observed among one, two or three DNA prime/rTV boost regimens. This study shows that one DNA prime/rTV boost regimen is sufficient to induce potent T cell responses against HIV.
