目的:探讨重组腺病毒p53基因(recombinant human Ad-p53,rAd-p53)治疗肝癌的抗肿瘤机制及临床应用。方法:应用PubMed、万方和CNKI数据库,以"肝癌、重组腺病毒p53基因、基因治疗、实验研究和临床应用"为关键词,检索1994-06-01-...目的:探讨重组腺病毒p53基因(recombinant human Ad-p53,rAd-p53)治疗肝癌的抗肿瘤机制及临床应用。方法:应用PubMed、万方和CNKI数据库,以"肝癌、重组腺病毒p53基因、基因治疗、实验研究和临床应用"为关键词,检索1994-06-01-2013-11-31文献739篇,纳入标准:1)p53基因与肝癌发生的相关性;2)重组腺病毒p53基因抗肿瘤机制;3)重组腺病毒p53治疗肝癌的实验研究;4)重组腺病毒p53治疗肝癌的临床应用研究。根据纳入标准选择符合分析的文献36篇。结果:p53基因是公认的肿瘤抑制基因,具有促使肿瘤细胞自杀和帮助缺陷细胞修复的作用。rAd-p53是复制缺陷型5型腺病毒载体与人p53基因重组的肿瘤基因治疗制品。rAd-p53通过多种途径发挥抗肿瘤作用,包括使肿瘤的细胞周期阻滞和发生程序性死亡,促进放射线对肿瘤细胞引起的细胞周期阻滞和凋亡,刺激机体产生抗肿瘤免疫反应,抑制肿瘤血管内皮生长因子,控制肿瘤的血管生成,使注射部位瘤组织产生局部血供障碍和坏死。此外,还能增加肝癌细胞的放化疗敏感性,间接增强抗肿瘤疗效。rAd-p53基因疗法为肝癌治疗开辟了崭新的途径,多项临床研究疗效良好。结论:rAd-p53基因对肿瘤的作用机制体现了在抗肿瘤治疗方面的有效性,并在基础研究及临床应用中均取得了显著进展,在肝癌的防治中将取得更大的突破。展开更多
p53 gene is a tumor suppressor gene and is involved in cell growth control.Wild type p53 gene was inserted into pCA13 and got recombinant plasmid pCAp53.293 cells(a kind of adenovirus packing cells)was co transfected ...p53 gene is a tumor suppressor gene and is involved in cell growth control.Wild type p53 gene was inserted into pCA13 and got recombinant plasmid pCAp53.293 cells(a kind of adenovirus packing cells)was co transfected with pCAp53 and pBHG11,recombinant p53 adenovirus was obtained.Virus particle can be seen under electric microscopy and its diameter is about 80 nm.Virus titer is 6.7×10 8 PFU/mL.Recombinant p53 adenovirus have no influence on growth of normal lung cells,but it can cause death of bladder tumor cell (EJ)and primary culture breast cancer cell.MTT assay results showed that recombinant p53 adenovirus inhibited growth of p53 deficient tumor cell,such as bladder tumor cell(EJ)and epidermis cancer cell(A431)and inhibiting rate in 72 hours may be 86.7% and 78.4%.Flow cytometry test showed that recombinant p53 adenovirus induced apoptosis of tumor cell.Wild type p53 gene mediated by adenovirus vector may be used in the treatment for cancer.展开更多
文摘目的:探讨重组腺病毒p53基因(recombinant human Ad-p53,rAd-p53)治疗肝癌的抗肿瘤机制及临床应用。方法:应用PubMed、万方和CNKI数据库,以"肝癌、重组腺病毒p53基因、基因治疗、实验研究和临床应用"为关键词,检索1994-06-01-2013-11-31文献739篇,纳入标准:1)p53基因与肝癌发生的相关性;2)重组腺病毒p53基因抗肿瘤机制;3)重组腺病毒p53治疗肝癌的实验研究;4)重组腺病毒p53治疗肝癌的临床应用研究。根据纳入标准选择符合分析的文献36篇。结果:p53基因是公认的肿瘤抑制基因,具有促使肿瘤细胞自杀和帮助缺陷细胞修复的作用。rAd-p53是复制缺陷型5型腺病毒载体与人p53基因重组的肿瘤基因治疗制品。rAd-p53通过多种途径发挥抗肿瘤作用,包括使肿瘤的细胞周期阻滞和发生程序性死亡,促进放射线对肿瘤细胞引起的细胞周期阻滞和凋亡,刺激机体产生抗肿瘤免疫反应,抑制肿瘤血管内皮生长因子,控制肿瘤的血管生成,使注射部位瘤组织产生局部血供障碍和坏死。此外,还能增加肝癌细胞的放化疗敏感性,间接增强抗肿瘤疗效。rAd-p53基因疗法为肝癌治疗开辟了崭新的途径,多项临床研究疗效良好。结论:rAd-p53基因对肿瘤的作用机制体现了在抗肿瘤治疗方面的有效性,并在基础研究及临床应用中均取得了显著进展,在肝癌的防治中将取得更大的突破。
文摘p53 gene is a tumor suppressor gene and is involved in cell growth control.Wild type p53 gene was inserted into pCA13 and got recombinant plasmid pCAp53.293 cells(a kind of adenovirus packing cells)was co transfected with pCAp53 and pBHG11,recombinant p53 adenovirus was obtained.Virus particle can be seen under electric microscopy and its diameter is about 80 nm.Virus titer is 6.7×10 8 PFU/mL.Recombinant p53 adenovirus have no influence on growth of normal lung cells,but it can cause death of bladder tumor cell (EJ)and primary culture breast cancer cell.MTT assay results showed that recombinant p53 adenovirus inhibited growth of p53 deficient tumor cell,such as bladder tumor cell(EJ)and epidermis cancer cell(A431)and inhibiting rate in 72 hours may be 86.7% and 78.4%.Flow cytometry test showed that recombinant p53 adenovirus induced apoptosis of tumor cell.Wild type p53 gene mediated by adenovirus vector may be used in the treatment for cancer.
