2001～2003款沃尔沃车系重设维修提示指示灯

提示:也可使用VADIS车辆通信重设指示灯. 1.重设维修提示灯(SRI),2001款车型 维修提示灯(SRI)说明车辆已到维修时间.该灯会在点火时保持亮起120秒.

Enhanced Production of Hybrid Extracellular β-Glucanase by Re-combinant Escherichia coli Using Experimental Design Method

A genetically engineered Escherichia coli JM109 harboring pLF3 was used to produce a hybrid ex-tracellular β-glucanase. Starting with enzyme production medium, glycerol and yeast extract combined with NaNO3 were screened to be the most suitable carbon and nitrogen source, respectively. Analysis of six components of the enzyme production medium by employing statistical optimization methods such as Plackett-Burman design and steepest ascent showed that yeast extract was the only significant variable and its best concentration for enzyme production was 12g·L-1. After optimization of the medium, 297.71U·ml-1 of β-glucanase activity in the medium and 352350U·g-1 of β-glucanase selectivity could be obtained, which were 14 and 72 folds higher than those ob-tained from original medium, respectively. Even higher enzyme activities were achieved by batch cultivations in a conventional stirred bioreactor on the optimized medium.

A Screening Program for Overweight Status Among Schoolgirls in Saudi Arabia： A Proposed Co-design Project to Tackle the Problem

The paper realizes the importance of communicating with schools to conduct nutritional screening, assessment and intervention programs for obesity and related risk factors to tackle the problem at earlier stage. The screening program was conducted by dietetics＇ students ＂under supervision＂ as part of their practice and community services to apply some assessment method for mritional status. Two intermediate schools for girls in Jeddah city were involved in the screening stage, followed by a full nutritional assessment and implementation of a designed intervention program at one of the schools. At screening stage, interview questionnaires for diet histories and anthropometric measurements used to screen overweight status and related food patterns. National and international references data used for assessment and comparison. Following screening approach, a designed comprehensive nutritional assessment and intervention program was piloted at one of the screened schools for overweight status. For screening stage： the overall prevalence of overweight girls is 61% （n = 81 out of 133） of the population, 73% （n = 91） of girls were centrally obese. Almost 3/4 of all students reported no participations in any type of physical activities/exercises. Dietary behaviors included non-consumption of breakfast （83%） and low intake of fruit and vegetables （38%）. For intervention stage, the present report concentrates on data concerning post intervention changes in Body Mass Index （BMI） and Waist Circumference （WC）. By the 30 days of intervention there were significant changes in values for BMI according to Saudi growth chart （p value = 0.009） with no changes among the control group. No statistically significant difference in the mean WC of the group. Study＇s results suggest that approaching young girls through schools to screen, assess and intervene overweight status and risk factors is vital. Although the non-random selection of only two schools for screening the prevalence of overweight status among school girls prevent the geralizability of the study findings to wider population, previous studies used random selection of schools and large sample sizes showed a high prevalence of overweight status among Saudi students Therefore, designing and piloting of an intervention program for implementation at a national level is useful to tackle theproblem.

Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodolog

Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was （377.9±1.7） mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gil2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.

Design of observer-based discrete repetitive-control system based on 2D model

A discrete observer-based repetitive control(RC) design method for a linear system with uncertainties was presented based on two-dimensional(2D) system theory. Firstly, a 2D discrete model was established to describe both the control behavior within a repetition period and the learning process taking place between periods. Next, by converting the designing problem of repetitive controller into one of the feedback gains of reconstructed variables, the stable condition was obtained through linear matrix inequality(LMI) and also the gain coefficient of repetitive system. Numerical simulation shows an exceptional feasibility of this proposal with remarkable robustness and tracking speed.