A population of 180 recombinant inbred lines （RILs） was developed by single seed descended from the cross of high yield Upland cotton （Gossypium hirsutum L.） varieties Zhongmiansuo12 （ZMSI2） and 8891, the two pa...A population of 180 recombinant inbred lines （RILs） was developed by single seed descended from the cross of high yield Upland cotton （Gossypium hirsutum L.） varieties Zhongmiansuo12 （ZMSI2） and 8891, the two parents of Xiangzamian2 （XZM2）. A genetic linkage map consisting of 132 loci and covering 865.20 cM was constructed using the RIL population chiefly with SSR markers. Yield and yield components were investigated for RILs in three environments in China. The purpose of the present research was to analyze the relationship between yield and its components and to map QTL for yield and yield components in cotton. QTL were tagged with data sets from single environment （separate analysis） and a set of data from means of the three environments （joint analysis）. A total of 34 QTL for yield and yield components were independently detected in three environments, whereas fifteen QTL were found in joint analysis. Notably, a stable lint percentage QTL qLP-A10-1 was detected both in joint analysis and in two environments of separate analysis, which might be of special value for marker-assisted selection. The QTL detected in the present study provide new information on improving yield and yield components. Results of path analysis showed that bolls/plant had the largest contribution to lint yield, which is consistent with the mid-parent heterosis value in F1. Accordingly, in cotton breeding, bolls/plant can be considered first and other yield components measured as a whole to implement variety enhancement and hybrid selection of cotton.展开更多
The present study examines affective meaning of pronouns （in Russian） represented by the semantic differential. Of direct relevance to the present study is the theory of affective meaning propounded by Osgood. Two h...The present study examines affective meaning of pronouns （in Russian） represented by the semantic differential. Of direct relevance to the present study is the theory of affective meaning propounded by Osgood. Two hypotheses were tested. According to a ＂magnitude＂ hypothesis, affective dimensions （each of evaluation, potency, and activity taken separately） differ in their magnitude across pronouns I, My, Me, and They. A ＂structural＂ hypothesis stated that the affective dimensions yield latent factors across （the generality） and within （the concept-scale interaction） the pronoun concepts. Repeated-measures analysis of variance （1-way ANOVA） and confirmatory factor analysis were employed to process data. It was found that each of the evaluation and potency measures yield a significant magnitude change across the pronouns, but there was indicated no significant change across the pronouns with respect to the activity dimension. Therewith, the pronoun My gained a salient value and the pronoun They the smallest value. Using confirmatory factor analysis five models were tested. Among them one model was good fit to the data. It engaged a four-factor solution resulted in that four pronouns are latent affective distinct but related factors and the evaluation, potency, and activity are their indicators.展开更多
PICKLE(PKL), a putative CHD3 chromatin remodeling factor, has been suggested to be involved in multiple processes in Arabidopsis. Here, we confirmed the late-flowering phenotype caused by pkl mutation with pkl mutants...PICKLE(PKL), a putative CHD3 chromatin remodeling factor, has been suggested to be involved in multiple processes in Arabidopsis. Here, we confirmed the late-flowering phenotype caused by pkl mutation with pkl mutants in two different ecotypes, and investigated the possible mechanisms that account for PKL regulation of flowering time. Quantitative RT-PCR and RNA-seq assays showed that expression of the LEAFY gene(LFY) and a number of LFY-regulated floral homeotic genes were down-regulated in seedlings of the pkl mutants. As predicted, overexpression of LFY restored normal flowering time of pkl mutants. Our results suggest that PKL may be involved in regulating flowering time via LFY expression. To uncover the underlying mechanism, Ch IP-PCR using anti-PKL was performed on materials from three developmental stages of seedlings. Our results showed that PKL associated with the genomic sequences of LFY, particularly at 10-day and 25-day after germination. We also showed that loss of PKL affected H3K27me3 level at the promoter of LFY. Taken together, our data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants.展开更多
As an research example of the widely existing cooperation-competition systems,the authorspresent an empirical investigation on a fruit nutritive factor network.It is described by a node-weightedbipartite graph.The fru...As an research example of the widely existing cooperation-competition systems,the authorspresent an empirical investigation on a fruit nutritive factor network.It is described by a node-weightedbipartite graph.The fruit nutritive factors are defined as the nodes,and two nodes are connected by anedge if at least one fruit contains these two nutritive factors.The fruits are defined as the collaborationacts.The node-weight W_(nt),which signifies the 'importance degree' of each actor node,is defined as thecont ent of a nutritive factor in a fruit.The empirical investigation results show some unique features.The node-weight distributions take so-called 'shifted power law' function forms,but the act-weightdistribution takes a normal form.The degree and act-degree distributions show impulsive-spectrumlike forms.These observations may be helpful for the study of fruits.The network description methodproposed in this article may be universal for a kind of cooperation-competition systems.展开更多
基金This work was supported by the National Science Foundation for Outstanding Youth Scholars (No. 30025029), Chinese National Programs for High Technology Research and Development (No. 2002AA207006), the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions, and Program for Changjiang Scholars and Innovative Research Team in University of MOE, China.
文摘A population of 180 recombinant inbred lines （RILs） was developed by single seed descended from the cross of high yield Upland cotton （Gossypium hirsutum L.） varieties Zhongmiansuo12 （ZMSI2） and 8891, the two parents of Xiangzamian2 （XZM2）. A genetic linkage map consisting of 132 loci and covering 865.20 cM was constructed using the RIL population chiefly with SSR markers. Yield and yield components were investigated for RILs in three environments in China. The purpose of the present research was to analyze the relationship between yield and its components and to map QTL for yield and yield components in cotton. QTL were tagged with data sets from single environment （separate analysis） and a set of data from means of the three environments （joint analysis）. A total of 34 QTL for yield and yield components were independently detected in three environments, whereas fifteen QTL were found in joint analysis. Notably, a stable lint percentage QTL qLP-A10-1 was detected both in joint analysis and in two environments of separate analysis, which might be of special value for marker-assisted selection. The QTL detected in the present study provide new information on improving yield and yield components. Results of path analysis showed that bolls/plant had the largest contribution to lint yield, which is consistent with the mid-parent heterosis value in F1. Accordingly, in cotton breeding, bolls/plant can be considered first and other yield components measured as a whole to implement variety enhancement and hybrid selection of cotton.
文摘The present study examines affective meaning of pronouns （in Russian） represented by the semantic differential. Of direct relevance to the present study is the theory of affective meaning propounded by Osgood. Two hypotheses were tested. According to a ＂magnitude＂ hypothesis, affective dimensions （each of evaluation, potency, and activity taken separately） differ in their magnitude across pronouns I, My, Me, and They. A ＂structural＂ hypothesis stated that the affective dimensions yield latent factors across （the generality） and within （the concept-scale interaction） the pronoun concepts. Repeated-measures analysis of variance （1-way ANOVA） and confirmatory factor analysis were employed to process data. It was found that each of the evaluation and potency measures yield a significant magnitude change across the pronouns, but there was indicated no significant change across the pronouns with respect to the activity dimension. Therewith, the pronoun My gained a salient value and the pronoun They the smallest value. Using confirmatory factor analysis five models were tested. Among them one model was good fit to the data. It engaged a four-factor solution resulted in that four pronouns are latent affective distinct but related factors and the evaluation, potency, and activity are their indicators.
文摘PICKLE(PKL), a putative CHD3 chromatin remodeling factor, has been suggested to be involved in multiple processes in Arabidopsis. Here, we confirmed the late-flowering phenotype caused by pkl mutation with pkl mutants in two different ecotypes, and investigated the possible mechanisms that account for PKL regulation of flowering time. Quantitative RT-PCR and RNA-seq assays showed that expression of the LEAFY gene(LFY) and a number of LFY-regulated floral homeotic genes were down-regulated in seedlings of the pkl mutants. As predicted, overexpression of LFY restored normal flowering time of pkl mutants. Our results suggest that PKL may be involved in regulating flowering time via LFY expression. To uncover the underlying mechanism, Ch IP-PCR using anti-PKL was performed on materials from three developmental stages of seedlings. Our results showed that PKL associated with the genomic sequences of LFY, particularly at 10-day and 25-day after germination. We also showed that loss of PKL affected H3K27me3 level at the promoter of LFY. Taken together, our data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants.
基金supported by the National Natural Science Foundation of China under Grant Nos. 10635040 and 70671089
文摘As an research example of the widely existing cooperation-competition systems,the authorspresent an empirical investigation on a fruit nutritive factor network.It is described by a node-weightedbipartite graph.The fruit nutritive factors are defined as the nodes,and two nodes are connected by anedge if at least one fruit contains these two nutritive factors.The fruits are defined as the collaborationacts.The node-weight W_(nt),which signifies the 'importance degree' of each actor node,is defined as thecont ent of a nutritive factor in a fruit.The empirical investigation results show some unique features.The node-weight distributions take so-called 'shifted power law' function forms,but the act-weightdistribution takes a normal form.The degree and act-degree distributions show impulsive-spectrumlike forms.These observations may be helpful for the study of fruits.The network description methodproposed in this article may be universal for a kind of cooperation-competition systems.
