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野生元蘑多糖的分离鉴定及其清除氧自由基作用 被引量:19
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作者 杨立红 刘林德 +3 位作者 姜华 王晓洁 邢伟 蔡德华 《食品科学》 EI CAS CSCD 北大核心 2007年第11期81-85,共5页
元蘑烘干后经热水提取,乙醇沉淀得粗多糖。粗多糖经Sevag法除蛋白后DEAE-纤维素(OH-)柱层析,分离得到两种多糖组份HsI和HsII,Sepharose CL-4B测得HsI和HsII分子量均为2.7725×104,红外光谱和NMR波普分析表明HsI和HsII均为含α和β... 元蘑烘干后经热水提取,乙醇沉淀得粗多糖。粗多糖经Sevag法除蛋白后DEAE-纤维素(OH-)柱层析,分离得到两种多糖组份HsI和HsII,Sepharose CL-4B测得HsI和HsII分子量均为2.7725×104,红外光谱和NMR波普分析表明HsI和HsII均为含α和β两种构型的D-吡喃糖葡聚糖,其α和β两种构型比值HsI为2:1和HsII为1:1。单糖的连接方式为1-4连接和1-6连接,其比值HsI为1:1和HsII为2:1。邻苯三酚自氧化法对其清除氧自由基作用测定结果表明,元蘑多糖粗品和纯品HsI和HsII均具有清除氧自由基作用。 展开更多
关键词 野生元蘑 多糖 分离鉴定 清除氧自由基
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电感耦合等离子体发射光谱法测定野生元蘑中铅的含量 被引量:1
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作者 梁婉婷 田鹏 +1 位作者 辛士刚 鲁捷 《山东化工》 CAS 2017年第2期54-55,共2页
采用ICP-AES测定了野生元蘑中Pb元素的含量。比较了湿法和干法硝解对实验结果的影响,计算得到相对标准偏差7.15%和5.72%,回收率在93.9%和96.2%。计算得到t值为2.32,表明两种方法无显著性的差异。
关键词 电感耦合等离子体发射光谱法 PB 野生元蘑
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The Content of Selenium and Other Ten Chemical Elements in Boletus Edulis from Bulgaria and Inhibition of Lipid Peroxidation
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作者 G. Bekyarov M. Kakalova +6 位作者 A. Iliev Tsv. Bekyarova G.V. Donchenko O.M. Palyvoda O.V. Vydiuk A.S.Dubovetskyi O.I. Kuzmenko 《Journal of Environmental Science and Engineering》 2011年第6期708-715,共8页
The goal of this study was to determine the content of such biological active metals as Se, Hg, AI, Cu, Zn, As, Cd, Pb, Mg, Ca and Fe in Boletus Edulis mushrooms and to study the effect of mushrooms as inhibitors of b... The goal of this study was to determine the content of such biological active metals as Se, Hg, AI, Cu, Zn, As, Cd, Pb, Mg, Ca and Fe in Boletus Edulis mushrooms and to study the effect of mushrooms as inhibitors of blood serum copper-initiated lipid peroxidation. The metals content was determined by ICP-OES technique and blood lipids peroxidation in vitro was assessed by thiobarbituric acid-reactive substances measurement. The dependency between quality and content of the determined biological active metals has been traced. Samples were analyzed of wild growing mushrooms Boletus Edulis from two mountain regions in Bulgaria. On the average the content of Se in Boletus Edulis was found to be 25 mg/kg dried mushroom, this content being higher in tubules than in fleshy part. We found that Boletus Edulis mushrooms inhibited lipid peroxidation in the concentration dependent manner. The effective concentration of Boletus Edulis is in 5 times lower compared to the concentration of Cantharellus Cibarius resulting in similar lipid peroxidation inhibition. This effect can be explained by 56 times higher content of Se and by 1.5 and 3 times lower content of such initiators of lipid peroxidation as Cu and Fe in Boletus Edulis compared to Cantharellus Cibarius. A system with a source of infrared radiation heating, developed by authors, was used for the mushroom mineralization. We conclude that Boletus Edulis is an effective inhibitor of blood lipid peroxidation and in 5 times stronger rather than Cantharellus Cibarius. 展开更多
关键词 Boletus edulis ICP-OES heavy metals SELENIUM lipid peroxidation ANTIOXIDANTS
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