[Objective] The research aimed to study the effects of soluble substance concentration in cell and the scavenging activity of active oxygen radicals on the cold-tolerance physiology of Dongxiang wild rice.[Method] Don...[Objective] The research aimed to study the effects of soluble substance concentration in cell and the scavenging activity of active oxygen radicals on the cold-tolerance physiology of Dongxiang wild rice.[Method] Dongxiang wild rice was used as materials to detect and compare the content of soluble sugar,free proline and soluble protein,POD activity,CAT activity,MDA content in the leaves in different growth periods.Using with control of two species of common cultivated rice Xieqingzao and Nongda 288.[Resul...展开更多
AIM: To investigate the effects of dendritic cells (DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response.METHODS: The wild-type p53 was transduced to DCs with ade...AIM: To investigate the effects of dendritic cells (DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response.METHODS: The wild-type p53 was transduced to DCs with adenovirus, and the DCs were stimulated by gastric cancer lysates. The surface molecules (B7-1, B7-2, MHC-I, MHC-II) of all DCs were detected by FACS, and the ability of the DCs to induce efficient and specific immunological response inanti-^51Cr-labeled target cells was studied. BALB/c mice injected with DCs and Mk28 were established, and CTL response in mice immunized with Lywt-p53DC was evaluated.Tumor-bearing mice were treated with Lywt-p53DC.RESULTS: The surface molecules of Lywt-p53DC had a high expression of B7-1 (86.70±0.07%), B7-2 (18.77±0.08%),MHC-I (87.20±0.05%) and MHC-II (56.70±0.07%); T lymphocytes had a specific CTL lysis ability induced by Lywt-p53DC; the CTL lysis rate was as high as 81%. The immune protection of Lywtp-53DC was obvious, the tumor diameter in Lywtp-53DC group was 3.10+0.31 ram, 2.73±0.23 ram,3.70±0.07 mm on d 13, 16 and 19, respectively, which were smaller than control, DC, wtp53DC and LyDC group (P<0.05). Tumor growth rate in Lywtp53DC group was slower than that in other groups (P<0.05).CONCLUSION: DCs transfected with wild-type p53 and stimulated by gastric cancer lysates have specific CTL killing activity.展开更多
It is of great importance for the analysis on genetic evolution mechanism, and rice breeding utilization by estimation and protection of genetic diversity of pre- sent DXWR (Oryza rufipogon Griff.). This study has s...It is of great importance for the analysis on genetic evolution mechanism, and rice breeding utilization by estimation and protection of genetic diversity of pre- sent DXWR (Oryza rufipogon Griff.). This study has summarized genetic diversity and protection achievements of DXWR from many aspects like varied conservations, years, generations, other species of wild rice and cultivated rice. In this study, a u- nified and scientific evaluation system of genetic diversity is encouraged to be built and suggestions on research prospect and ecological conservation of DXWR genetic diversity have been proposed.展开更多
[Objective] The flowering,fruiting and seed setting characteristic of wild watermelon were discussed in our paper.[Method] The flowering and fruiting habit of wild watermelon were observed,and the effect of different ...[Objective] The flowering,fruiting and seed setting characteristic of wild watermelon were discussed in our paper.[Method] The flowering and fruiting habit of wild watermelon were observed,and the effect of different pollination methods(mixed pollination,single flower pollination and natural pollination)on fruit set percentage,weight per fruit,seeds per fruit and thousand-seed weight of wild watermelon were studied in our paper.[Result] Wild watermelon had few female flowers and low fruit set percentage;artificial pollination could not increase the fruit set percentage of wild watermelon obviously,but it could improve weight per fruit,seed yield and quality significantly,and mixed pollination was the best.[Conclusion] Our study could provide reference for the improvement of hybrid seed yield and quality of wild watermelon.展开更多
Low temperature and sparse light in early spring is one of the factors causing reduction for rice production. So it is important to develop cold tolerance cultivars. In the present study, cold tolerance characters of ...Low temperature and sparse light in early spring is one of the factors causing reduction for rice production. So it is important to develop cold tolerance cultivars. In the present study, cold tolerance characters of 36 parents and 423 rice lines from 68 hybrid groups were investigated at seedling stage under low tempera- ture and sparse light conditions in field. There were 10 parent accessions with strong cold tolerance of level 1. Among them, 3 were common wild rice accessions; 4 were japonica rice cultivars; 2 were indica rice; 1 was offspring from hybrid be- tween indica and japonica.There were 33 lines with strong cold tolerance of level 1 from the hybrid groups of common wild rice,and 15 from the hybrid groups of IRBB5, and only 3 from the hybrid groups of BPHR96. There were abundant cold tolerance resources in rice germplasm. It was feasible to develop cold tolerance cul- tivars from the hybrids among common wild rice, japonica cultivars and indica culti- vars.展开更多
Akebia is a wild fruit originating in the Yangtse river valley and the surrounding areas. It includes mainly three species (subspecies): Akebia trifoliata, Akebia trifoliata subsp, australis, and Akebia quinata. It...Akebia is a wild fruit originating in the Yangtse river valley and the surrounding areas. It includes mainly three species (subspecies): Akebia trifoliata, Akebia trifoliata subsp, australis, and Akebia quinata. It has many good traits, such as large fruit size, early fruit-setting and high yield, excellent eating quality, and good environmental adaptability. However, there are some shortcomings with the fruit, including thick skin, excessive number of seeds, and relatively small proportion of edible flesh. Akebia is also a traditional Chinese medicine, which has the functions of relieving internal heat and diuresis, dredging venation and improving blood circulation, cleansing facial chloasma, detoxication and anti-cancer activities. Akebia can be used as fresh fruit, for processing, and for pharmaceutical purposes. We have been undertaking a series of research on the domestication and utilization of Akebia, including cultivar selection, techniques for cultivation, storage and processing, and related basic studies. A Chinese trade name “Hehuanguo” and an English trade name “Joyfruit” have been registered for Akebia to facilitate its commercial development.展开更多
基金National Research Center for Wildlife-borne Diseases,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101.Meng Meng:Doctor,Northeast Forest ry University,Harbin 150040China Wildlife Conservation Association,Beijing 100714.
基金Supported by National Natural Science Foundation(30660087)Natural Science Foundation of Jiangxi Province(0630034)+1 种基金Jiangxi Provincial Science and Technology Agency"Industrial research"Project(Ganke[2004]211)Jiangxi Provincial Department of Education Science and Technology Project(Ganjiaoji[2006]138)~~
文摘[Objective] The research aimed to study the effects of soluble substance concentration in cell and the scavenging activity of active oxygen radicals on the cold-tolerance physiology of Dongxiang wild rice.[Method] Dongxiang wild rice was used as materials to detect and compare the content of soluble sugar,free proline and soluble protein,POD activity,CAT activity,MDA content in the leaves in different growth periods.Using with control of two species of common cultivated rice Xieqingzao and Nongda 288.[Resul...
文摘AIM: To investigate the effects of dendritic cells (DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response.METHODS: The wild-type p53 was transduced to DCs with adenovirus, and the DCs were stimulated by gastric cancer lysates. The surface molecules (B7-1, B7-2, MHC-I, MHC-II) of all DCs were detected by FACS, and the ability of the DCs to induce efficient and specific immunological response inanti-^51Cr-labeled target cells was studied. BALB/c mice injected with DCs and Mk28 were established, and CTL response in mice immunized with Lywt-p53DC was evaluated.Tumor-bearing mice were treated with Lywt-p53DC.RESULTS: The surface molecules of Lywt-p53DC had a high expression of B7-1 (86.70±0.07%), B7-2 (18.77±0.08%),MHC-I (87.20±0.05%) and MHC-II (56.70±0.07%); T lymphocytes had a specific CTL lysis ability induced by Lywt-p53DC; the CTL lysis rate was as high as 81%. The immune protection of Lywtp-53DC was obvious, the tumor diameter in Lywtp-53DC group was 3.10+0.31 ram, 2.73±0.23 ram,3.70±0.07 mm on d 13, 16 and 19, respectively, which were smaller than control, DC, wtp53DC and LyDC group (P<0.05). Tumor growth rate in Lywtp53DC group was slower than that in other groups (P<0.05).CONCLUSION: DCs transfected with wild-type p53 and stimulated by gastric cancer lysates have specific CTL killing activity.
基金Supported by National Natural Science Foundation of China(30960189)the National Public Benefit(Agricultural)Research Foundation of China(201103007)+1 种基金Special Funds for Establishment of Modern Agricultural and Industrial Technology System(NXCYTY-01)Project supported by Jiangxi Academy of Agricultural Science for Innovation(2010CQN008)~~
文摘It is of great importance for the analysis on genetic evolution mechanism, and rice breeding utilization by estimation and protection of genetic diversity of pre- sent DXWR (Oryza rufipogon Griff.). This study has summarized genetic diversity and protection achievements of DXWR from many aspects like varied conservations, years, generations, other species of wild rice and cultivated rice. In this study, a u- nified and scientific evaluation system of genetic diversity is encouraged to be built and suggestions on research prospect and ecological conservation of DXWR genetic diversity have been proposed.
基金Supported by Scientific Research Foundation of Yangtze University(HNZDi200329)~~
文摘[Objective] The flowering,fruiting and seed setting characteristic of wild watermelon were discussed in our paper.[Method] The flowering and fruiting habit of wild watermelon were observed,and the effect of different pollination methods(mixed pollination,single flower pollination and natural pollination)on fruit set percentage,weight per fruit,seeds per fruit and thousand-seed weight of wild watermelon were studied in our paper.[Result] Wild watermelon had few female flowers and low fruit set percentage;artificial pollination could not increase the fruit set percentage of wild watermelon obviously,but it could improve weight per fruit,seed yield and quality significantly,and mixed pollination was the best.[Conclusion] Our study could provide reference for the improvement of hybrid seed yield and quality of wild watermelon.
基金Supported by Guangxi Natural Science Foundation Item,China(2013GXNSFBA019-066)Guangxi Science Research and Technology Development Project,China(Guikezhong14121001-2-4)Nanning Municipal Science Research and Technology Development Plan Item,China(20132304)~~
文摘Low temperature and sparse light in early spring is one of the factors causing reduction for rice production. So it is important to develop cold tolerance cultivars. In the present study, cold tolerance characters of 36 parents and 423 rice lines from 68 hybrid groups were investigated at seedling stage under low tempera- ture and sparse light conditions in field. There were 10 parent accessions with strong cold tolerance of level 1. Among them, 3 were common wild rice accessions; 4 were japonica rice cultivars; 2 were indica rice; 1 was offspring from hybrid be- tween indica and japonica.There were 33 lines with strong cold tolerance of level 1 from the hybrid groups of common wild rice,and 15 from the hybrid groups of IRBB5, and only 3 from the hybrid groups of BPHR96. There were abundant cold tolerance resources in rice germplasm. It was feasible to develop cold tolerance cul- tivars from the hybrids among common wild rice, japonica cultivars and indica culti- vars.
文摘Akebia is a wild fruit originating in the Yangtse river valley and the surrounding areas. It includes mainly three species (subspecies): Akebia trifoliata, Akebia trifoliata subsp, australis, and Akebia quinata. It has many good traits, such as large fruit size, early fruit-setting and high yield, excellent eating quality, and good environmental adaptability. However, there are some shortcomings with the fruit, including thick skin, excessive number of seeds, and relatively small proportion of edible flesh. Akebia is also a traditional Chinese medicine, which has the functions of relieving internal heat and diuresis, dredging venation and improving blood circulation, cleansing facial chloasma, detoxication and anti-cancer activities. Akebia can be used as fresh fruit, for processing, and for pharmaceutical purposes. We have been undertaking a series of research on the domestication and utilization of Akebia, including cultivar selection, techniques for cultivation, storage and processing, and related basic studies. A Chinese trade name “Hehuanguo” and an English trade name “Joyfruit” have been registered for Akebia to facilitate its commercial development.