利用C基因组对高杆野生稻和宽叶野生稻基因组的比较分析(英文)

[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution.

野败型育性恢复基因在AA基因组野生稻中的分布与遗传

分析了野败型恢复基因在AA基因组野生稻的分布。结果表明:(1)在31份野生稻中,有16份含有恢复基因,分布频率达5 1 .6 %。(2 ) 6个AA基因组野生稻种中有4个种存在恢复基因,但主要集中于O rufipogon和O nivara。(3)在所鉴定的16份野生稻恢复系中,对野败型花粉育性恢复力大于80 %和5 0 %～80 %的各6份,小于5 0 %的4份;强恢复源主要来自印度次大陆的一年生野生稻O nivara中。(4)在随机选择的8份野生稻中,除w15含双基因外,其他的都只含有1对野败型恢复基因;对其中的6份野生稻的等位性分析表明。