糙毛假地豆(Desmodium heterocarpum DC var. strigosum Van Meeuwen)是多年生豆科草本植物,在广东省郁南县自然条件下零星分布,未形成大面积种群。研究表明,该植物适应光照能力强,适于荫蔽林下生长;其叶片冬季具有较高的叶绿素含量及...糙毛假地豆(Desmodium heterocarpum DC var. strigosum Van Meeuwen)是多年生豆科草本植物,在广东省郁南县自然条件下零星分布,未形成大面积种群。研究表明,该植物适应光照能力强,适于荫蔽林下生长;其叶片冬季具有较高的叶绿素含量及一定的净光合能力,且根部仍具有固N活性根瘤;根系发达,地上部分形成密实覆盖层,与其他杂草竞争能力强,具有多种生态功能,在开发利用当地生物资源中具有较高的应用价值。展开更多
[Objective] This study aimed to preliminarily determine the physiological mechanism of glyphosate resistance produced in wild soybean（Glycine soja） and further provide a basis for the breeding of glyphosate-resistan...[Objective] This study aimed to preliminarily determine the physiological mechanism of glyphosate resistance produced in wild soybean（Glycine soja） and further provide a basis for the breeding of glyphosate-resistant soybean. [Method] First,a screening for glyphosate resistant varieties among sixty-seven wild soybean materials was done in a field trial; subsequently, physiological indexes of the screened resistant variety ZYD0685 and the sensitive variety ZYD0790 were studied. [Result]At the glyphosate dose of 1.23 kg a.i/hm2, glyphoaste resistance varied greatly among different wild soybean materials, with the highest survival rate of 87% and83% occurring in ZYD0685 and ZYD2405, respectively, and that of another seven accessions ranged from 2.7% to 38%, and all the remaining fifty-eight soybean materials died. After treatment with glyphoaste at different doses, there were no significant differences in chlorophyll content and shikimate content in the resistant ZYD0685, but there was an evident increase in the activity of gultathione-S-transferases（GSTs）; while in the sensitive ZYD0790, the content of shikimic acid increased significantly, and chlorophyll content decreased significantly, and GSTs activity revealed a slight change. [Conclusion] Therefore, lowering the amount of accumulated shikimic acid is the major physiological response to glyphosate in wild soybean.展开更多
Wild soybean （Glycine soja） is the wild relative species of cultivated soy- bean, which is of high protein content, strong tolerance and high propagation coeffi- cient. The diverse growing conditions of wild soybean...Wild soybean （Glycine soja） is the wild relative species of cultivated soy- bean, which is of high protein content, strong tolerance and high propagation coeffi- cient. The diverse growing conditions of wild soybean in China contribute to numer- ous phenotypes of wild soybean, which also reflect the evolution process through long-term natural and artificial selection. The research progress of wild soybean germpfasms was summarized in resource status and innovative utilization in order to provide information for research of wild soybean germplasms.展开更多
Wild soybean is recognized as the relative wild species of cultivate soy- bean. China is rich in wild soybean resources. In the past 30 years, domestic scholars have carried out a large quantity of studies on the gene...Wild soybean is recognized as the relative wild species of cultivate soy- bean. China is rich in wild soybean resources. In the past 30 years, domestic scholars have carried out a large quantity of studies on the genetic diversity of wild soybean in China, which have laid the theoretical foundation for the protection and utilization of wild soybean resources. In this paper, the research progress on the genetic diversity of wild soybean in China at the phenotypic, biochemical and molecular levels of wild soybean was reviewed, and the future development of the research on the genetic diversity of wild soybean was introduced.展开更多
A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for...A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for 5 circles, with the distance of 3 m, from one circle to another. Then the wild soybean was planted in plots as the rays of the circles in 8 directions (N, E, W, S, NE, NW, SE and SW), spaced every 5 m until 50 m. Each plot comprised 25 plants. In the second year, the wild soybean seeds from the first year were planted in the field together with the original wild soybean as check. Before flowering time, high concentrations of roundups (about 4-5 times of the normal dose) were sprayed on the plants and the surviving plants were identified. The leaves were taken to the lab for DNA extraction to determine the unique DNA for roundup-ready soybean (CTAB method). About 2% of the plants survived, but some leaves were yellow. One plant of wild soybean was found to have the roundup-ready gene from the original roundup-ready soybean. The other surviving wild soybeans should also had some fragments of the roundup tolerance gene. However, the DNA bands were not very clear in the PCR map.展开更多
Glycine soja or wild soybean is the wild ancestor of the soybean, being an important source for property improvement and variety breeding. With abundant wild soybeans, their habitat is undermining, and wild soybeans a...Glycine soja or wild soybean is the wild ancestor of the soybean, being an important source for property improvement and variety breeding. With abundant wild soybeans, their habitat is undermining, and wild soybeans are facing increasing- ly threatened in Shandong if measures are not taken yet. Besides, wild soybean germplasm is far from further exploiting or using. The research analyzed status quo and problems of wild soybean germplasm posed countermeasures or suggestions in crop germplasm conservation and use. resources conservation and use and pro- order to provide references for agricultural crop germplasm conservation and use.展开更多
The circadian rhythms of apical meristem mitosis cells of the two types of soybean were investigated： wild soybeans - Glycine max （L.） Merr. （G. hispida Max.）, G. soja Sieb., and Zucc （G, ussuriensis Reg. and Ma...The circadian rhythms of apical meristem mitosis cells of the two types of soybean were investigated： wild soybeans - Glycine max （L.） Merr. （G. hispida Max.）, G. soja Sieb., and Zucc （G, ussuriensis Reg. and Maack）; and the hybrids of their homozygous forms （F6）. Representatives of the initial kinds and interspecific hybrids for reproduction were grown up in comparable conditions. Seeds of a soybean were grown up at 24℃ in Petri cups in thermostat in darkness during winter. For research of mitotic meristem cages activity the tips of roots were fixed temporal by acetoalcohol （fractional fixing） from 1 p.m. till 12 a.m., further up to 12 p.m. each 60 minutes. Acetokarmin was used for preparing the coloring. For researching of mitotic indexes, there have been analyzed from 6 up to 10 thousand cells at each stage （during each moment of time： 01 hour, 02, 03 06 ... 12, 13 ... 18 hours and further）. Within a day （day ＋ night） in cells of root apical meristem, three mitosis ＂waves＂ in each of the investigated forms with an absolute starting point of rest at 12 p.m., with precisely expressed further periodicity were found out. Each of the investigated forms misses in character of wave processes, on a degree of increase and recession of mitosis waves, on the level of mitotic indexes. Maximal mitotic activity at all the investigated forms is noted at 6 and 12 a.m. and 6 p.m.展开更多
As an important plant species with high protein contents,wild soybean(Glycine soja),has drawn much attention and appeared to be useful for the genetic improvement of soybean germplasms.Since temperature is one of the ...As an important plant species with high protein contents,wild soybean(Glycine soja),has drawn much attention and appeared to be useful for the genetic improvement of soybean germplasms.Since temperature is one of the numerous environmental factors affecting the germination of most plants,an experimental study was carried out to determine the effect of temperature on germination of wild soybean(G.soja)seeds.Germination test was conducted by setting up thirty-six constant and alternating temperature regimes,ranging from 5 to 40 ℃(16 h night/8 h day).Responses in germination rate to these temperature regimes were then used to construct a quadratic response surface,giving estimated germination rates with confidence intervals at P ≤ 0.05.The results showed that germination capacity was significantly greater while exposed to constant temperatures of 25 ℃,and under the alternating temperature regime the optimum temperature occurred at the 20/25,25/25,25/30 ℃ regime(16 h/8 h)with the amplitude widened from 0 to 5 ℃.Together with regional monthly climate data,these results could be used to improve and promote the cultivation of wild soybean(G.soja),making it available to develop the location-specific optimum seeding time and to apply weed-control treatments.展开更多
The research focuses on the study of anatomical and morphological stalk structure ofsoya interspecific hybrids of the third generation （F3） between （Glycine max （L.） Merr.） and G. soja Sieb. et Zucc. in comparis...The research focuses on the study of anatomical and morphological stalk structure ofsoya interspecific hybrids of the third generation （F3） between （Glycine max （L.） Merr.） and G. soja Sieb. et Zucc. in comparison with parent plant species. The parent plant species and interspecific hybrids were sowed and grew under similar conditions. The similarity of the anatomic structure of stalks of cultivated plants and Glycine soja （wild soya） proves the hypothesis the studied species have the same origin. However, the obtained results show the considerable degree of phylogenetic dissociation between the studied soya species. Interspecific hybrids inherit from G. soja the ability to high intensive growth. The G. soja use in practical selective breeding is of great interest.展开更多
文摘糙毛假地豆(Desmodium heterocarpum DC var. strigosum Van Meeuwen)是多年生豆科草本植物,在广东省郁南县自然条件下零星分布,未形成大面积种群。研究表明,该植物适应光照能力强,适于荫蔽林下生长;其叶片冬季具有较高的叶绿素含量及一定的净光合能力,且根部仍具有固N活性根瘤;根系发达,地上部分形成密实覆盖层,与其他杂草竞争能力强,具有多种生态功能,在开发利用当地生物资源中具有较高的应用价值。
基金Supported by the National Natural Science Foundation of China(30971834)~~
文摘[Objective] This study aimed to preliminarily determine the physiological mechanism of glyphosate resistance produced in wild soybean（Glycine soja） and further provide a basis for the breeding of glyphosate-resistant soybean. [Method] First,a screening for glyphosate resistant varieties among sixty-seven wild soybean materials was done in a field trial; subsequently, physiological indexes of the screened resistant variety ZYD0685 and the sensitive variety ZYD0790 were studied. [Result]At the glyphosate dose of 1.23 kg a.i/hm2, glyphoaste resistance varied greatly among different wild soybean materials, with the highest survival rate of 87% and83% occurring in ZYD0685 and ZYD2405, respectively, and that of another seven accessions ranged from 2.7% to 38%, and all the remaining fifty-eight soybean materials died. After treatment with glyphoaste at different doses, there were no significant differences in chlorophyll content and shikimate content in the resistant ZYD0685, but there was an evident increase in the activity of gultathione-S-transferases（GSTs）; while in the sensitive ZYD0790, the content of shikimic acid increased significantly, and chlorophyll content decreased significantly, and GSTs activity revealed a slight change. [Conclusion] Therefore, lowering the amount of accumulated shikimic acid is the major physiological response to glyphosate in wild soybean.
基金Supported by the Research and Demonstration of Green Transformation and New Agricultural Industry Mode in the Saline Alkali Land of the Yellow River Data,Key Research and Development (Industrial Key Technology) Project of Shandong Province(2016CYJS05A02)~~
文摘Wild soybean （Glycine soja） is the wild relative species of cultivated soy- bean, which is of high protein content, strong tolerance and high propagation coeffi- cient. The diverse growing conditions of wild soybean in China contribute to numer- ous phenotypes of wild soybean, which also reflect the evolution process through long-term natural and artificial selection. The research progress of wild soybean germpfasms was summarized in resource status and innovative utilization in order to provide information for research of wild soybean germplasms.
基金山东省农业科学院青年科研基金项目(2014QNM36)山东省农业科学院农业科技创新工程项目(CXGC2016A02/A12)+8 种基金国家绿肥产业技术体系(CARS-22)"十二五"国家科技支撑计划(2013BAD01B0106)山东省现代农业产业技术体系"杂粮创新团队"(SDAIT-15-01)国家农作物种质资源共享服务平台"山东子平台"(NICGR2015-032)Supported by the Scientific Research Funds for Young Scholars of Shandong Acdemy of Agricultural Sciences(2014QNM36)Agricultural Science & Technology Innovation Program of Shandong Academy of Agricultural Sciences(CXGC2016A02/A12)China Agriculture Research System-Green Manure(CARS-22)the National Science and Technology Support Program in the Twelfth FiveYear Plan Period of China(2013BAD01B0106)Shandong Province Modern Agricultural Industry Technology System"Coarse Cereal Innovation Team"(SDAIT-15-01)"Shandong Sub-platform"of National Crop Germplasm Resource Sharing Service Platform(NICGR2015-032)
文摘Wild soybean is recognized as the relative wild species of cultivate soy- bean. China is rich in wild soybean resources. In the past 30 years, domestic scholars have carried out a large quantity of studies on the genetic diversity of wild soybean in China, which have laid the theoretical foundation for the protection and utilization of wild soybean resources. In this paper, the research progress on the genetic diversity of wild soybean in China at the phenotypic, biochemical and molecular levels of wild soybean was reviewed, and the future development of the research on the genetic diversity of wild soybean was introduced.
文摘A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for 5 circles, with the distance of 3 m, from one circle to another. Then the wild soybean was planted in plots as the rays of the circles in 8 directions (N, E, W, S, NE, NW, SE and SW), spaced every 5 m until 50 m. Each plot comprised 25 plants. In the second year, the wild soybean seeds from the first year were planted in the field together with the original wild soybean as check. Before flowering time, high concentrations of roundups (about 4-5 times of the normal dose) were sprayed on the plants and the surviving plants were identified. The leaves were taken to the lab for DNA extraction to determine the unique DNA for roundup-ready soybean (CTAB method). About 2% of the plants survived, but some leaves were yellow. One plant of wild soybean was found to have the roundup-ready gene from the original roundup-ready soybean. The other surviving wild soybeans should also had some fragments of the roundup tolerance gene. However, the DNA bands were not very clear in the PCR map.
基金Supported by National Science&Technology Pillar Program during the Twelfth Fiveyear Plan Period(2013BAD01B0106)Sub-platform in Shandong Province of National Infrastructure of Plant Germplasm Resources(NICGR2015/2016-032)+2 种基金Shandong Provicne Modern Agricultural Technology System Coarse Cereals Innovation Team(SDAIT-15-01)Youth Foundation of Shandong Academy of Agricultural Sciences(2016YQN19)Agricultural Science&Technology Innovation Program of Shandong Academy of Agricultural Sciences(CXGC2016A02/A12)~~
文摘Glycine soja or wild soybean is the wild ancestor of the soybean, being an important source for property improvement and variety breeding. With abundant wild soybeans, their habitat is undermining, and wild soybeans are facing increasing- ly threatened in Shandong if measures are not taken yet. Besides, wild soybean germplasm is far from further exploiting or using. The research analyzed status quo and problems of wild soybean germplasm posed countermeasures or suggestions in crop germplasm conservation and use. resources conservation and use and pro- order to provide references for agricultural crop germplasm conservation and use.
文摘The circadian rhythms of apical meristem mitosis cells of the two types of soybean were investigated： wild soybeans - Glycine max （L.） Merr. （G. hispida Max.）, G. soja Sieb., and Zucc （G, ussuriensis Reg. and Maack）; and the hybrids of their homozygous forms （F6）. Representatives of the initial kinds and interspecific hybrids for reproduction were grown up in comparable conditions. Seeds of a soybean were grown up at 24℃ in Petri cups in thermostat in darkness during winter. For research of mitotic meristem cages activity the tips of roots were fixed temporal by acetoalcohol （fractional fixing） from 1 p.m. till 12 a.m., further up to 12 p.m. each 60 minutes. Acetokarmin was used for preparing the coloring. For researching of mitotic indexes, there have been analyzed from 6 up to 10 thousand cells at each stage （during each moment of time： 01 hour, 02, 03 06 ... 12, 13 ... 18 hours and further）. Within a day （day ＋ night） in cells of root apical meristem, three mitosis ＂waves＂ in each of the investigated forms with an absolute starting point of rest at 12 p.m., with precisely expressed further periodicity were found out. Each of the investigated forms misses in character of wave processes, on a degree of increase and recession of mitosis waves, on the level of mitotic indexes. Maximal mitotic activity at all the investigated forms is noted at 6 and 12 a.m. and 6 p.m.
基金supported by the fund of Jinhua Science Technology Foundation of China(2009-2-02)
文摘As an important plant species with high protein contents,wild soybean(Glycine soja),has drawn much attention and appeared to be useful for the genetic improvement of soybean germplasms.Since temperature is one of the numerous environmental factors affecting the germination of most plants,an experimental study was carried out to determine the effect of temperature on germination of wild soybean(G.soja)seeds.Germination test was conducted by setting up thirty-six constant and alternating temperature regimes,ranging from 5 to 40 ℃(16 h night/8 h day).Responses in germination rate to these temperature regimes were then used to construct a quadratic response surface,giving estimated germination rates with confidence intervals at P ≤ 0.05.The results showed that germination capacity was significantly greater while exposed to constant temperatures of 25 ℃,and under the alternating temperature regime the optimum temperature occurred at the 20/25,25/25,25/30 ℃ regime(16 h/8 h)with the amplitude widened from 0 to 5 ℃.Together with regional monthly climate data,these results could be used to improve and promote the cultivation of wild soybean(G.soja),making it available to develop the location-specific optimum seeding time and to apply weed-control treatments.
文摘The research focuses on the study of anatomical and morphological stalk structure ofsoya interspecific hybrids of the third generation （F3） between （Glycine max （L.） Merr.） and G. soja Sieb. et Zucc. in comparison with parent plant species. The parent plant species and interspecific hybrids were sowed and grew under similar conditions. The similarity of the anatomic structure of stalks of cultivated plants and Glycine soja （wild soya） proves the hypothesis the studied species have the same origin. However, the obtained results show the considerable degree of phylogenetic dissociation between the studied soya species. Interspecific hybrids inherit from G. soja the ability to high intensive growth. The G. soja use in practical selective breeding is of great interest.
