Under the threat of bio-terrorism,this paper aims at improving the emergency rescue system's ability of dealing with a public health emergency.Focusing on the demand network,this research establishes the emergency...Under the threat of bio-terrorism,this paper aims at improving the emergency rescue system's ability of dealing with a public health emergency.Focusing on the demand network,this research establishes the emergency rescue supply storage network under a danger diffusion environment.Combined with the infectious disease diffusion model,the traditional set covering model is rebuilt taking the decision optimization of reserve quantity into consideration.Under the premise of a certain emergency service level,the collaborative location optimizing model of an emergency rescue supply storage network is established in order to minimize the sum of the building costs and the preserving costs.The model is proved to be effective through numerical simulation.The collaborative location optimization of the nodes of the emergency rescue supply storage network and the reserve quantity of each storage node is realized.展开更多
The effects of Z11 and AP407 collectors as well as AF65 and AF70 frothers were evaluated in the rougher flotation circuit of the Sungun copper concentrator plant using 2 4 full factorial design.Response functions were...The effects of Z11 and AP407 collectors as well as AF65 and AF70 frothers were evaluated in the rougher flotation circuit of the Sungun copper concentrator plant using 2 4 full factorial design.Response functions were produced for both Cu grade and recovery and optimized within the experimental range.The optimum reagent dosages were found to be 12.01 g/t Z11,11 g/t AP407,3 g/t AF65 and 5 g/t AF70 to attain the maximum Cu grade(8.17%).The reagent dosages of 12 g/t Z11,11 g/t AP407,3 g/t AF65 and 15 g/t AF70 produced the maximum Cu recovery(86.44%).The collector distribution demonstrated that the distribution pattern of(32%,32%,20%,16%)can produce the best recovery(87.75%)in comparison to other examined distribution patterns.展开更多
Abstract： A simple optimized microplate-based method to assay endo-1,4-β-mannosidase activity was described as an improved high-throughput screening method. A series of experimental conditions were optimized. It is ...Abstract： A simple optimized microplate-based method to assay endo-1,4-β-mannosidase activity was described as an improved high-throughput screening method. A series of experimental conditions were optimized. It is revealed that the optimum measurement procedure is as follows： adding 50μL of diluted enzyme sample and 50 μL substrate, incubating at 45 ℃ for exactly 5 min in micro-plate, mixing with 100 μL 3,5-dinitrosalicylic acid （DNS） reagent, maintaining at boiling point for 15 rain, cooling down to room temperature before determining the ABS value at 540 nm using an ELISA micro-plate reader. The reaction volume of the optimized microplate-assay is reduced to 200μL from 2 500 μL used in the standard β-mannanase macro-assay. The optimized micro-assay is significantly more sensitive in all of the 643 candidates during endo-1,4-β-mannosidase screening. Statistical analyses show that the sensitivity of the optimized micro-method is significantly greater than that of the macro-assay. The optimized method is convenient, fast, and cheap for high throughput enzyme screening.展开更多
According to the problem of energy consumption in wireless sensor network （WSN）,this paper puts forward a routing optimization algorithm with quality of multi-service, using the function of routing optimization with...According to the problem of energy consumption in wireless sensor network （WSN）,this paper puts forward a routing optimization algorithm with quality of multi-service, using the function of routing optimization with quality of multi-service and membership function of satisfaction, which integrates the energy consumption of communication and residual and the information of time delay into the membership function of satisfaction to solve the equilibrium factor, so that it can become the optimal routing that balances the network lifetime, transmission delay of data, and node energy consumption of nodes. Simulation experiment shows that adopting the algorithm can make lifecycle of nodes longer and network transmit more data packets at the same time. Experimental results verify the algorithm can effectively balance the network energy, reduce the energy consumption and prolong the network lifetime.展开更多
基金The National Natural Science Foundation of China(No.70671021)the National Key Technology R&D Program of China during the11th Five-Year Plan Period(No.2006BAH02A06)
文摘Under the threat of bio-terrorism,this paper aims at improving the emergency rescue system's ability of dealing with a public health emergency.Focusing on the demand network,this research establishes the emergency rescue supply storage network under a danger diffusion environment.Combined with the infectious disease diffusion model,the traditional set covering model is rebuilt taking the decision optimization of reserve quantity into consideration.Under the premise of a certain emergency service level,the collaborative location optimizing model of an emergency rescue supply storage network is established in order to minimize the sum of the building costs and the preserving costs.The model is proved to be effective through numerical simulation.The collaborative location optimization of the nodes of the emergency rescue supply storage network and the reserve quantity of each storage node is realized.
文摘The effects of Z11 and AP407 collectors as well as AF65 and AF70 frothers were evaluated in the rougher flotation circuit of the Sungun copper concentrator plant using 2 4 full factorial design.Response functions were produced for both Cu grade and recovery and optimized within the experimental range.The optimum reagent dosages were found to be 12.01 g/t Z11,11 g/t AP407,3 g/t AF65 and 5 g/t AF70 to attain the maximum Cu grade(8.17%).The reagent dosages of 12 g/t Z11,11 g/t AP407,3 g/t AF65 and 15 g/t AF70 produced the maximum Cu recovery(86.44%).The collector distribution demonstrated that the distribution pattern of(32%,32%,20%,16%)can produce the best recovery(87.75%)in comparison to other examined distribution patterns.
基金Project(31000350)supported by the National Natural Science Foundation of China
文摘Abstract： A simple optimized microplate-based method to assay endo-1,4-β-mannosidase activity was described as an improved high-throughput screening method. A series of experimental conditions were optimized. It is revealed that the optimum measurement procedure is as follows： adding 50μL of diluted enzyme sample and 50 μL substrate, incubating at 45 ℃ for exactly 5 min in micro-plate, mixing with 100 μL 3,5-dinitrosalicylic acid （DNS） reagent, maintaining at boiling point for 15 rain, cooling down to room temperature before determining the ABS value at 540 nm using an ELISA micro-plate reader. The reaction volume of the optimized microplate-assay is reduced to 200μL from 2 500 μL used in the standard β-mannanase macro-assay. The optimized micro-assay is significantly more sensitive in all of the 643 candidates during endo-1,4-β-mannosidase screening. Statistical analyses show that the sensitivity of the optimized micro-method is significantly greater than that of the macro-assay. The optimized method is convenient, fast, and cheap for high throughput enzyme screening.
文摘According to the problem of energy consumption in wireless sensor network （WSN）,this paper puts forward a routing optimization algorithm with quality of multi-service, using the function of routing optimization with quality of multi-service and membership function of satisfaction, which integrates the energy consumption of communication and residual and the information of time delay into the membership function of satisfaction to solve the equilibrium factor, so that it can become the optimal routing that balances the network lifetime, transmission delay of data, and node energy consumption of nodes. Simulation experiment shows that adopting the algorithm can make lifecycle of nodes longer and network transmit more data packets at the same time. Experimental results verify the algorithm can effectively balance the network energy, reduce the energy consumption and prolong the network lifetime.
