Vibrio parahaemolyticus is the leading causal agent of human acute gas- troenteritis. Real-time accurate detection means is the key to prevention and control of its spread. This study provided a novel detection strate...Vibrio parahaemolyticus is the leading causal agent of human acute gas- troenteritis. Real-time accurate detection means is the key to prevention and control of its spread. This study provided a novel detection strategy for realizing rapid and specific determination of V. parahaemolyticus by labeling its monoclonal antibody （Ab） with quantum dots （QDs）. The results showed that the fluorescence of these QDs-Ab bioconjugates was quenched by graphene oxide （GO） to produce a bacteri- um capture probe. And the optimal quenched concentration of GO was 60 ng/ml. When the bacterium capture probe was exposed to the target, green color fluores- cence was turned on by releasing the QDs-Ab due to the antibody antigen combi- nation. The detection limit of V. parahaemolyticus was 104 CFU/ml based on 3 times signal-to-noise ratio. The specificity of the FRET sensor towards V. para- haemolyticus was examined by comparing with controls such as V. splendidus, V. alginolyticus, Edwardsiella tarda and Aeromonas hydrophila with the same condition. The controls couldn＇t cause obvious fluorescence alteration, while the target resulted in significant fluorescence enhancement. This strategy could be further used as a universal method for any bacterial determination by changing the conjugated antibod- ies in early disease diagnosis. Therefore, the sensor has good potential to expand its application to the early diagnosis and determination of bacteria.展开更多
In this paper, we mainly study the preparation of an optical biosensor based on porous silicon(PSi) Bragg mirror and its feasibility for biological detection. The quantum dot(QD) labeled biotin was pipetted onto strep...In this paper, we mainly study the preparation of an optical biosensor based on porous silicon(PSi) Bragg mirror and its feasibility for biological detection. The quantum dot(QD) labeled biotin was pipetted onto streptavidin functionalized PSi Bragg mirror samples, the affinity reaction between QD labeled biotin and streptavidin in PSi occurred, so the QDs were indirectly connected to the PSi. The fluorescence of QD enhanced the signal of biological reactions in PSi. The performance of the sensor is verified by detecting the fluorescence of the QD in PSi. Due to the fluorescence intensity of the QDs can be enhanced by PSi Bragg mirror, the sensitivity of the PSi optical biosensor will be improved.展开更多
基金Supported by Shandong Scientific and Technological Development Program(2014GHY115024)~~
文摘Vibrio parahaemolyticus is the leading causal agent of human acute gas- troenteritis. Real-time accurate detection means is the key to prevention and control of its spread. This study provided a novel detection strategy for realizing rapid and specific determination of V. parahaemolyticus by labeling its monoclonal antibody （Ab） with quantum dots （QDs）. The results showed that the fluorescence of these QDs-Ab bioconjugates was quenched by graphene oxide （GO） to produce a bacteri- um capture probe. And the optimal quenched concentration of GO was 60 ng/ml. When the bacterium capture probe was exposed to the target, green color fluores- cence was turned on by releasing the QDs-Ab due to the antibody antigen combi- nation. The detection limit of V. parahaemolyticus was 104 CFU/ml based on 3 times signal-to-noise ratio. The specificity of the FRET sensor towards V. para- haemolyticus was examined by comparing with controls such as V. splendidus, V. alginolyticus, Edwardsiella tarda and Aeromonas hydrophila with the same condition. The controls couldn＇t cause obvious fluorescence alteration, while the target resulted in significant fluorescence enhancement. This strategy could be further used as a universal method for any bacterial determination by changing the conjugated antibod- ies in early disease diagnosis. Therefore, the sensor has good potential to expand its application to the early diagnosis and determination of bacteria.
基金supported by the National Natural Science Foundation of China(Nos.61575168 and 61665012)the Xinjiang Science and Technology Project(No.201412112)
文摘In this paper, we mainly study the preparation of an optical biosensor based on porous silicon(PSi) Bragg mirror and its feasibility for biological detection. The quantum dot(QD) labeled biotin was pipetted onto streptavidin functionalized PSi Bragg mirror samples, the affinity reaction between QD labeled biotin and streptavidin in PSi occurred, so the QDs were indirectly connected to the PSi. The fluorescence of QD enhanced the signal of biological reactions in PSi. The performance of the sensor is verified by detecting the fluorescence of the QD in PSi. Due to the fluorescence intensity of the QDs can be enhanced by PSi Bragg mirror, the sensitivity of the PSi optical biosensor will be improved.
