By using ‘history reconstruction method’,this paper studied the dynamics of two Polytrichum juniperinum populations with and without sporophytes.The population with sporophyte production experienced a dynamic proces...By using ‘history reconstruction method’,this paper studied the dynamics of two Polytrichum juniperinum populations with and without sporophytes.The population with sporophyte production experienced a dynamic process from increase to stabilization,while that without sporophyte production experienced a process from increase to decrease.Before 2003,both the increase rate and the birth rate of two populations decreased continuously,with the death rate showing the tendency from decrease to a continuous increase.The advantages of ’history reconstruction method’ in studying population dynamics were proved and discussed.展开更多
目的:建立金发藓科植物ISSR-PCR反应的最佳体系。方法:利用正交设计的方法,对金发藓科植物(Polytrichaceae)IS-SR-PCR反应的5因素(Mg2+,dNTP,primer,DNA template,Taq DNA polymerase)4水平进行试验。结果:在20μl反应体系中,模板DNA50n...目的:建立金发藓科植物ISSR-PCR反应的最佳体系。方法:利用正交设计的方法,对金发藓科植物(Polytrichaceae)IS-SR-PCR反应的5因素(Mg2+,dNTP,primer,DNA template,Taq DNA polymerase)4水平进行试验。结果:在20μl反应体系中,模板DNA50ng,1.6μmol/L的引物,1×反应缓冲液,3.2mmol/L的Mg2+,dNTP为1.2mmol/L,2U的TaqDNA聚合酶,反应程序为94℃预变性6min;94℃变性45s,57℃退火45s,72℃延伸2min,循环40次;72℃延伸10min。利用此结论,对20种金发藓科植物进行ISSR-PCR扩增,扩增产物的多态性为69.52%。利用引物841构建的指纹图谱,可区分20种金发藓科植物中的18种,分辨率达90%。金发藓科植物ISSR-PCR反应体系的建立,为今后利用ISSR标记技术开展金发藓科植物种间遗传多样性分析提供一个标准化程序。展开更多
文摘By using ‘history reconstruction method’,this paper studied the dynamics of two Polytrichum juniperinum populations with and without sporophytes.The population with sporophyte production experienced a dynamic process from increase to stabilization,while that without sporophyte production experienced a process from increase to decrease.Before 2003,both the increase rate and the birth rate of two populations decreased continuously,with the death rate showing the tendency from decrease to a continuous increase.The advantages of ’history reconstruction method’ in studying population dynamics were proved and discussed.
文摘目的:建立金发藓科植物ISSR-PCR反应的最佳体系。方法:利用正交设计的方法,对金发藓科植物(Polytrichaceae)IS-SR-PCR反应的5因素(Mg2+,dNTP,primer,DNA template,Taq DNA polymerase)4水平进行试验。结果:在20μl反应体系中,模板DNA50ng,1.6μmol/L的引物,1×反应缓冲液,3.2mmol/L的Mg2+,dNTP为1.2mmol/L,2U的TaqDNA聚合酶,反应程序为94℃预变性6min;94℃变性45s,57℃退火45s,72℃延伸2min,循环40次;72℃延伸10min。利用此结论,对20种金发藓科植物进行ISSR-PCR扩增,扩增产物的多态性为69.52%。利用引物841构建的指纹图谱,可区分20种金发藓科植物中的18种,分辨率达90%。金发藓科植物ISSR-PCR反应体系的建立,为今后利用ISSR标记技术开展金发藓科植物种间遗传多样性分析提供一个标准化程序。