金叶紫穗槐抗寒生理研究

以二年生金叶紫穗槐(Amorpha fruticosa L.cv.Jinye)和紫穗槐(对照)枝条为对象,分别测定自然降温下叶片的电导率和丙二醛、可溶性糖、可溶性蛋白、脯氨酸的含量以及人工低温下的SOD、POD、CAT活性的变化,探讨金叶紫穗槐在低温条件下的适应能力。结果表明,随着自然温度的降低,金叶紫穗槐的丙二醛含量低于对照;渗透调节物质可溶性糖、可溶性蛋白和脯氨酸含量升高,比对照有更强的低温耐受能力;SOD、POD、CAT活性持续升高,高于对照,抵御低温的能力优于对照。不过金叶紫穗槐的相对电导率与对照的差异不显著。

金叶紫穗槐组织培养研究

以金叶紫穗槐(Amorpha fruticosa‘Jinye’)为外植体,探讨了不同生长调节对外植体分化、增殖和生根的影响。结果表明,(1)金叶紫穗槐组织培养最适宜的外植体是半木质化带芽茎段,以70%乙醇30 s+0.1%升汞(HgCl_2)消毒4~5 min为最好,外植体发芽率可达66.7%~70.0%。(2)适宜的增殖培养基为MS+6-BA 0.2 mg/L+NAA 0.01 mg/L,适宜的生根培养基为1/2MS+IBA 0.5 mg/L。(3)生根试管苗适宜的开瓶炼苗时间为2~3 d,在一年中的3~5月,选择基径≥0.3 mm的试管苗移栽到蛭石培养基上能够获得93.3%的成活率。

金叶紫穗槐水培生根研究

为了探索金叶紫穗槐(Amorpha fruticosa‘Jinye’)的生根特性,以一年生金叶紫穗槐枝条为研究材料,对其进行了不同浓度生根剂(ABT1号生根粉、ABT2号生根粉、华诺联邦生根粉)、不同直径(D≥1.0cm、0.5 cm<D<1.0 cm、D≤0.5 cm)插穗的水培生根研究。结果表明,华诺联邦生根粉适宜浓度为200 mg/L,生根率最高达97.5%,ABT1号、ABT2号生根粉的适宜浓度为100 mg/L,生根率分别可达93.1%、90.5%。金叶紫穗槐插穗直径与水培生根率呈正相关,与水培生根时间呈负相关。金叶紫穗槐插穗D≥1.0 cm、0.5 cm<D<1.0 cm、D≤0.5 cm的生根率分别为100%、97.5%、80.8%,生根时间分别为8、12、18 d。金叶紫穗槐不定根的产生位置为侧芽基部及愈伤组织,节间不生根。

金叶紫穗槐育苗技术研究

以金叶紫穗槐为研究对象,论述了金叶紫穗槐的压条、扦插(嫩枝扦插、硬枝扦插)育苗、嫁接育苗技术要点,为科学繁育金叶紫穗槐提供了依据。

Physiology of Cold Resistance of Amorpha fruticosa L. cv. Jinye

With two-year-old Amorpha fruticosa L. cv. Jinye and A. fruticosa as the research objects, the relative conductivity, MDA content, soluble sugar content, solu- ble protein content and proline content in leaf were determined during natural drop in temperature, and the SOD, POD and COD activity in leaf were determined under artificial low temperature to explore the adaptability of A. fruticosa L. cv. Jinye to low temperature. The results showed that with the decrease in natural temperature, the content of MDA in A. fruticosa L. cv. Jinye was lower than that in the control, and the contents of osmotic adjustment substances （soluble sugar, soluble protein and proline） increased, indicating that A. fruticosa L. cv. Jinye had a stronger toler- ance to low temperature compared with A. fruticosa. The activity of SOD, POD and CAT in A. fruticosa L. cv. Jinye increased continuously, higher than that in the con- trol, indicating that the resisting ability of A. fruticosa L. cv. Jinye against low tem- perature was superior to that of the control. There was no significant difference in relative conductivity between A. fruticosa L. cv. Jinye and A. fruticosa.