Objective： To observe the effect of acupuncture plus thunder-fire moxibustion on the expressions of matrix metalloproteinase-3 （MMP-3）, tissue inhibitor of metalloproteinase-1 （TIMP-1） and transforming growth fac...Objective： To observe the effect of acupuncture plus thunder-fire moxibustion on the expressions of matrix metalloproteinase-3 （MMP-3）, tissue inhibitor of metalloproteinase-1 （TIMP-1） and transforming growth factor-β1 （TGF-β1） in cartilage of knee osteoarthritis （KOA） rats, and to explore the mechanism of acupuncture plus thunder-fire moxibustion in the treatment of KOA. Methods： Thirty Sprague-Dawley （SD） rats were randomly divided into a blank control group, a model group and an acupuncture-moxibustion group by random digits table, 10 rats in each group. Rats in the model group and the acupuncture-moxibustion group were injected with papain in the right posterior knee joint to prepare the models. The levels of MMP-3 and TIMP-1 in rat synovium of each group were measured by enzyme-linked immunosorbent assay （ELBA） after 2 weeks of treatment. The level of TGF-β1 was determined by Motic B5 Micro-camera system. Results： The levels of MMP-3 and TIMP-1 in the cartilage of the model group were significantly higher than those in the blank control group （all P〈0.01）; the levels of MMP-3 and TIMP-1 in the acupuncture-moxibustion group were lower than those in the model group, and the between-group differences were statistically significant （all P〈0.05）. The levels of MMP-3 and TIMP-1 in the acupuncture-moxibustion group were higher than those in the blank control group, and the differences were statistically significant （all P〈0.05）. The level of TGF-β1 in cartilage tissues of the model group was significantly lower than that in the blank control group （P〈0.01）; the level of TGF-β1 in the acupuncture-moxibustion group was higher than that in the model group （P〈0.05）, but it was lower than that in the blank control group, and the between-group difference was statistically significant （P〈0.05）. Conclusion： Acupuncture plus thunder-fire moxibustion can effectively recover the abnormal expressions of MMP-3 and TIMP-1 in KOA model rats and somewhat up-regulate TGF-β1, which may be one of its mechanisms of acupuncture plus thunder-fire for KOA.展开更多
基金supported by Project of State Administration of Traditional Chinese Medicine, No. LP0118041~~
文摘Objective： To observe the effect of acupuncture plus thunder-fire moxibustion on the expressions of matrix metalloproteinase-3 （MMP-3）, tissue inhibitor of metalloproteinase-1 （TIMP-1） and transforming growth factor-β1 （TGF-β1） in cartilage of knee osteoarthritis （KOA） rats, and to explore the mechanism of acupuncture plus thunder-fire moxibustion in the treatment of KOA. Methods： Thirty Sprague-Dawley （SD） rats were randomly divided into a blank control group, a model group and an acupuncture-moxibustion group by random digits table, 10 rats in each group. Rats in the model group and the acupuncture-moxibustion group were injected with papain in the right posterior knee joint to prepare the models. The levels of MMP-3 and TIMP-1 in rat synovium of each group were measured by enzyme-linked immunosorbent assay （ELBA） after 2 weeks of treatment. The level of TGF-β1 was determined by Motic B5 Micro-camera system. Results： The levels of MMP-3 and TIMP-1 in the cartilage of the model group were significantly higher than those in the blank control group （all P〈0.01）; the levels of MMP-3 and TIMP-1 in the acupuncture-moxibustion group were lower than those in the model group, and the between-group differences were statistically significant （all P〈0.05）. The levels of MMP-3 and TIMP-1 in the acupuncture-moxibustion group were higher than those in the blank control group, and the differences were statistically significant （all P〈0.05）. The level of TGF-β1 in cartilage tissues of the model group was significantly lower than that in the blank control group （P〈0.01）; the level of TGF-β1 in the acupuncture-moxibustion group was higher than that in the model group （P〈0.05）, but it was lower than that in the blank control group, and the between-group difference was statistically significant （P〈0.05）. Conclusion： Acupuncture plus thunder-fire moxibustion can effectively recover the abnormal expressions of MMP-3 and TIMP-1 in KOA model rats and somewhat up-regulate TGF-β1, which may be one of its mechanisms of acupuncture plus thunder-fire for KOA.
