An electrochemical quantitative method of serum albumin was developed based on the orange G(OG)as probe.In pH 2.0 Britton-Robinson(B-R)buffer solution,OG has a sensitive reductive peak at -0.27V(vs.SCE)on the hanging ...An electrochemical quantitative method of serum albumin was developed based on the orange G(OG)as probe.In pH 2.0 Britton-Robinson(B-R)buffer solution,OG has a sensitive reductive peak at -0.27V(vs.SCE)on the hanging mercury drop electrode.After adding human serum albumin(HSA)to the above solution,the reduction peak current of OG decreases apparently without the shift of the peak potential.The decrease of the reduction peak current of OG is in linear with HSA concentration from 4.0 to 28.0mg/L with the regression equation △ip″(nA)=85.02+15.93C(mg/L),γ=0.993.This method was further applied to the determination of the content of HSA in blood samples and the results were in accordance with the traditional Coomassie brilliant blue G-250 spectrophotometric assay.展开更多
文摘An electrochemical quantitative method of serum albumin was developed based on the orange G(OG)as probe.In pH 2.0 Britton-Robinson(B-R)buffer solution,OG has a sensitive reductive peak at -0.27V(vs.SCE)on the hanging mercury drop electrode.After adding human serum albumin(HSA)to the above solution,the reduction peak current of OG decreases apparently without the shift of the peak potential.The decrease of the reduction peak current of OG is in linear with HSA concentration from 4.0 to 28.0mg/L with the regression equation △ip″(nA)=85.02+15.93C(mg/L),γ=0.993.This method was further applied to the determination of the content of HSA in blood samples and the results were in accordance with the traditional Coomassie brilliant blue G-250 spectrophotometric assay.
